Human Calpain-5(CAPN5) ELISA kit

Instructions
Code CSB-EL004498HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name calpain 5
Alternative Names ADNIV ELISA Kit; Calpain htra 3 ELISA Kit; Calpain htra-3 ELISA Kit; Calpain-5 ELISA Kit; CAN5_HUMAN ELISA Kit; CAPN 5 ELISA Kit; Capn5 ELISA Kit; nCL 3 ELISA Kit; nCL-3 ELISA Kit; New calpain 3 ELISA Kit; VRNI ELISA Kit
Abbreviation CAPN5
Uniprot No. O15484
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CAPN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %81-91
1:2Average %105
Range %99-108
1:4Average %89
Range %85-93
1:8Average %93
Range %89-97
Recovery
The recovery of human CAPN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9184-95
EDTA plasma (n=4)9589-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20001.972 1.917 1.945 1.787
10001.339 1.358 1.349 1.191
5000.979 0.956 0.968 0.810
2500.728 0.712 0.720 0.562
1250.448 0.454 0.451 0.293
62.50.381 0.369 0.375 0.217
31.250.261 0.277 0.269 0.111
00.160 0.156 0.158
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Calcium-regulated non-lysosomal thiol-protease.
Gene References into Functions
  1. The relative domain rotation of 60-100 degrees we found for mini-calpain-5 (a non-classical calpain) is significantly greater than the largest rotation previously observed for a classical calpain PMID: 27474374
  2. CAPN5 localization at the photoreceptor synapse and with mitochondria explains the neural circuitry phenotype in human CAPN5 disease alleles. PMID: 27152965
  3. A novel CAPN5 (c.750G>T, p.Lys250Asn) missense mutation causes uveitis and neovascular retinal detachment. PMID: 25856303
  4. CAPN5 mutation in hereditary uveitis: the R243L mutation increases calpain catalytic activity and triggers intraocular inflammation PMID: 25994508
  5. CAPN5 expression can be suppressed by shRNA-based RNA interference PMID: 25216694
  6. autosomal dominant neovascular inflammatory vitreoretinopathy is due to CAPN5 gain-of-function rather than haploinsufficiency. PMID: 24381307
  7. Calpain-5 mutations cause autoimmune uveitis, retinal neovascularization, and photoreceptor degeneration. PMID: 23055945
  8. Results describe the cloning and characterization of rat calpain-5, which is highly homologous to human and mouse sequences. PMID: 15464980
  9. CAPN5 polymorphisms are associated with a risk of polycystic ovary syndrome. PMID: 16396936
  10. CAPN5 seems to influence traits related to increased risk for cardiovascular diseases and play a role as a candidate gene for metabolic syndrome PMID: 17227582
  11. We have found significant interaction between CAPN5 and PPARD genes that reduces risk for obesity in 55%. CAPN5 and PPARD gene products may also interact in vivo. PMID: 18657264
  12. Data examine possible allelic imbalance in papillary thyroid cancer at EMSY, CAPN5, and PAK1, as candidate genes within 11q13.5-q14 region using a single nucleotide polymorphism-based analysis. PMID: 18787380
  13. Calpain5 was expressed in endometrial stromal and glandular cells throughout the menstrual cycle and in decidua, and its expression was decreased in both stromal and glandular cells from women with endometriosis compared with that of fertile controls. PMID: 18829447

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Involvement in disease Vitreoretinopathy, neovascular inflammatory (VRNI)
Protein Families Peptidase C2 family
Tissue Specificity Expressed in many tissues. Strong expression in the photoreceptor cells of the retina, with a punctate pattern of labeling over the nuclei and inner segments with less expression along the other segments and outer plexiform layer.
Database Links

HGNC: 1482

OMIM: 193235

KEGG: hsa:726

STRING: 9606.ENSP00000278559

UniGene: Hs.248153

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