Human Carboxymethyl lysine,CML ELISA Kit

Instructions
Code CSB-E12798h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Carboxymethyl lysine,CML
Abbreviation CML
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CML in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %82-95
1:2Average %94
Range %90-100
1:4Average %95
Range %91-105
1:8Average %94
Range %85-100
Recovery
The recovery of human CML spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9285-98
EDTA plasma (n=4)9491-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.372 2.252 2.312 2.162
20001.775 1.660 1.718 1.568
10001.160 1.142 1.151 1.001
5000.712 0.699 0.706 0.556
2500.493 0.456 0.475 0.325
1250.342 0.329 0.336 0.186
62.50.264 0.249 0.257 0.107
00.153 0.147 0.150  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

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 Q&A
Q:

Does this kit measure the total or protein bound cml?

A:
Thanks for your inquiry. CSB-E12798h detects protein bound CML.Pls let me know if you have any further questions. Thank you.
Q:

We have planned to assess the abundance of cml in protein samples, collected from cell cultures, using the human CML ELISA kit CSB-E12798h. What kind of lysis buffer is recommended to use to collect CML in cell samples? We are used to lyse cell with T-PER/Protease inhibitor cocktail or Igepal at 0.5%. Are they compatible with the purchased CML Elisa kit (CSB-E12798h)

A:
Thanks for your inquiry.
From your description, you want to make detection on cell lysis, here advice you to use the sample method on our attached manual.
As for the method you mentioned, so sorry that it is not verified from our side.
If your sample has been prepared, pls arrange preliminary experiment to check.
The enzyme proportion should be combined with the enzyme preparation protocol.
And dear, we have 24T trial kit, if you have interest, advice you to have this small size for try.
Any question, pls feel free to contact me.Pls let me know if you have any further questions. Thank you.

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