Human Carboxymethyl lysine,CML ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

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Human Carboxymethyl lysine,CML ELISA Kit

Name: Human Carboxymethyl lysine,CML ELISA Kit
Brand: CUSABIO
SKU: CSB-E12798h

Citations (10) Protocol MSDS

Code	CSB-E12798h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details
Citations (10)
Customer Reviews and Q&A

Target Name

Carboxymethyl lysine,CML

Abbreviation

CML

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CML in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-95
1:2	Average %	94
	Range %	90-100
1:4	Average %	95
	Range %	91-105
1:8	Average %	94
	Range %	85-100

Recovery

The recovery of human CML spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-98
EDTA plasma (n=4)	94	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
4000	2.372	2.252	2.312	2.162
2000	1.775	1.660	1.718	1.568
1000	1.160	1.142	1.151	1.001
500	0.712	0.699	0.706	0.556
250	0.493	0.456	0.475	0.325
125	0.342	0.329	0.336	0.186
62.5	0.264	0.249	0.257	0.107
0	0.153	0.147	0.150

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human CML ELISA Kit was designed for the quantitative measurement of Human CML protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

Protective effect of Inonotus obliquus polysaccharide on MGO-induced nonenzymatic glycation fibroblasts C Chen,Heliyon,2024
Serum Advanced Glycation End Products and Their Soluble Receptor as New Biomarkers in Systemic Lupus Erythematosus I Carrión,Biomedicines,2024
Protective effect of Inonotus obliquus polysaccharide on fibroblasts after MGO-induced nonenzymatic glycation C Chen,/,2023
Advanced Glycation end Products and Bone Metabolism in Patients With Chronic Kidney Disease KRS Quadros,JBMR Plus,2023
The role of advanced glycation end products in vascular aging: which parameter is the most suitable as a biomarker? O Mayer,Journal of Human Hypertension,2020
Methylglyoxal and Glyoxal as Potential Peripheral Markers for MCI Diagnosis and Their Effects on the Expression of Neurotrophic, Inflammatory and Neurodegenerative Factors in Neurons and in Neuronal Derived-Extracellular Vesicles Haddad M, et al,International Journal of Molecular Sciences,2019
Circulating and Extracellular Vesicles Levels of N-(1-Carboxymethyl)-L-Lysine (CML) Differentiate Early to Moderate Alzheimer's Disease Haddad M,journal of alzheimers disease,2019
Reduction Levels and the Effects of High-Molecular-Weight Adiponectin via AMPK/eNOS in Chinese Type 2 Diabetes Lian K.et al,Exp Clin Endocrinol Diabetes.,2016
The effects of n-3 long-chain polyunsaturated fatty acid supplementation on AGEs and sRAGE in type 2 diabetes mellitus Kurt A.et al,J Physiol Biochem.,2016
Pomegranate (Punicagranatum) juiceDecreases lipid peroxidation, but has no effect on plasma advanced glycated end-products in adults with type 2 Sohrab G. et al,Food Nutr Res,2015

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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■ Q&A

Does this kit measure the total or protein bound cml?

Thanks for your inquiry. CSB-E12798h detects protein bound CML.Pls let me know if you have any further questions. Thank you.

We have planned to assess the abundance of cml in protein samples, collected from cell cultures, using the human CML ELISA kit CSB-E12798h. What kind of lysis buffer is recommended to use to collect CML in cell samples? We are used to lyse cell with T-PER/Protease inhibitor cocktail or Igepal at 0.5%. Are they compatible with the purchased CML Elisa kit (CSB-E12798h)

Thanks for your inquiry.
From your description, you want to make detection on cell lysis, here advice you to use the sample method on our attached manual.
As for the method you mentioned, so sorry that it is not verified from our side.
If your sample has been prepared, pls arrange preliminary experiment to check.
The enzyme proportion should be combined with the enzyme preparation protocol.
And dear, we have 24T trial kit, if you have interest, advice you to have this small size for try.
Any question, pls feel free to contact me.Pls let me know if you have any further questions. Thank you.

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