Human Chondroitin sulfate proteoglycan 4(CSPG4) ELISA kit

Code CSB-EL006076HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chondroitin sulfate proteoglycan 4
Alternative Names 4732461B14Rik ELISA Kit; AN2 ELISA Kit; AN2 proteoglycan ELISA Kit; Chondroitin sulfate proteoglycan 4 (melanoma-associated) ELISA Kit; Chondroitin sulfate proteoglycan 4 ELISA Kit; Chondroitin sulfate proteoglycan NG2 ELISA Kit; Cspg4 ELISA Kit; Cspg4 chondroitin sulfate proteoglycan 4 ELISA Kit; CSPG4_HUMAN ELISA Kit; HMW-MAA ELISA Kit; HSN tumor-specific antigen ELISA Kit; Kiaa4232 ELISA Kit; MCSP ELISA Kit; MCSPG ELISA Kit; MEL-CSPG ELISA Kit; Melanoma chondroitin sulfate proteoglycan ELISA Kit; Melanoma-associated chondroitin sulfate proteoglycan ELISA Kit; MELCSPG ELISA Kit; MSK16 ELISA Kit; NG2 ELISA Kit
Abbreviation CSPG4
Uniprot No. Q6UVK1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CSPG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %87-95
1:2Average %89
Range %83-94
1:4Average %92
Range %89-95
1:8Average %97
Range %92-103
Recovery
The recovery of human CSPG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9691-102
EDTA plasma (n=4)8882-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.170 2.134 2.152 1.958
8001.501 1.602 1.552 1.358
4001.011 1.089 1.050 0.856
2000.737 0.725 0.731 0.537
1000.474 0.463 0.469 0.275
500.341 0.355 0.348 0.154
250.263 0.273 0.268 0.074
00.193 0.194 0.194
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
4.0 - 1 reviews

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Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: No dilution

Review: I used CSB-EL006076HU ELISA kit to quantify Chondroitine Sulphate both after intestinal passage (on CaCo-2 cells) and after entering in the chondrocyte (T/C-28a2). This kit allowed to accurately measure the presence of chondroitin in our samples and for this reason I would suggest to buy it.

By Anonymous

 Q&A
Q:

Could you tell me which sequence of the protein is recognised by the antibody of your kit ?

A:
Thanks for your inquiry!Pls check the antibody information for this kit:
CSB-EL006076HU
Coated anti: Mouse monoclonal antibody
Detect anti: Goat polyclonal antibody

Target Background

Function
(From Uniprot)
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Gene References into Functions
  1. DNA methylation status of blood leukocytes may be associated with susceptibility to Colorectal Cancer . The MCSM-H of blood leukocytes may be associated with CRC risk, especially in younger people. PMID: 29291617
  2. This study revealed that CSPG4 interacted with perlecan to support cell adhesion and actin polymerization and the data suggest a novel mechanism by which CSPG4 expressing cells might attach to perlecan-rich matrices so as those found in connective tissues and basement membranes. PMID: 29462330
  3. Both tumor cell and vascular NG2 expression were shown to be present in a significant number of patients with colorectal cancer and this makes NG2 a double target for anti-tumor therapies. PMID: 28715802
  4. NG2 may represent a promising target for the modulation of ICAM-1-mediated immune responses. PMID: 28964848
  5. In addition to establishing the binding region for CSPG4, this work ascribes for the first time a role in TcdB CROPs in receptor binding and further clarifies the relative roles of host receptors in TcdB pathogenesis. PMID: 28842504
  6. of NG2/CSPG4 rather than changes in CD44 or Ki-67 expression is associated with low overall survival in glioblastoma multiforme patients PMID: 28945172
  7. The results indicate that CSPG4/NG2 has roles in regulating chondrosarcoma cell function in relation to growth, spread and resistance to chemotherapy. PMID: 27292772
  8. A positive CSPG4 stain may be associated with an increased risk of metastasis and mortality from chordoma. PMID: 26689475
  9. CSPG4 is a cell surface proteoglycan regulated by interleukin 11 in human endometrial epithelial cancer cells. the data suggest that CSPG4 inhibition may impair endometrial cancer progression by reducing cancer cell proliferation, migration and potentially epithelial-to-mesenchymal transition. PMID: 28098860
  10. These data suggest the possible involvement of GD3 and NG2 in pre/pro-tumorigenic events occurring in the complex microenvironment of the tissue surrounding glioblastoma PMID: 26792897
  11. the ratio of serum proteoglycan 4 to protease C1 inhibitor may be used for screening of early breast cancer. PMID: 26890881
  12. The NG2 proteoglycan protects oligodendrocyte precursor cells against oxidative stress via interaction with OMI/HtrA2. PMID: 26340347
  13. The up-regulation of NG2 is associated with poor prognosis in HCC. PMID: 26074703
  14. Low CSPG4 expression is associated with pancreatic tumors. PMID: 24932730
  15. These data are consistent with the concept that expression of inhibitory CSPG within the injury scar is an important impediment to regeneration but that NG2+ progenitors derived from ESNLCs can modify the microenvironment PMID: 25713464
  16. NG2 expression in pediatric brain tumors differs depending upon type and, unlike adult glioma, includes expression on lower-grade tumors. PMID: 25503117
  17. The results highlight an unprecedented, complex pattern of NG2/CSPG4 isoform expression in foetal and neoplastic CNS PMID: 24386429
  18. MMP-13 overexpression or exogenous MMP-13 reduces anoikis by more effectively shedding NG2. PMID: 25166220
  19. Reduced promoter methylation and increased expression of CSPG4 are associated with head and neck squamous cell carcinomas. PMID: 24740185
  20. T-cells transduced with a CSPG4-specific chimeric antigen receptor inhibit the growth of CSPG4-expressing tumor cells both in vitro and in vivo. PMID: 24334762
  21. Describe CSPG4-specific immunotoxin that kills rhabdomyosarcoma cells and binds to primary tumor tissues. PMID: 25016058
  22. NG2 knockdown results in loss of beta1 integrin activation in endothelial cells, revealing a mechanism for NG2-dependent cross talk between pericytes and endothelial cells. PMID: 23925489
  23. data suggest that locally produced CSPG4 stimulates human extravillous trophoblast migration and invasion and suggests that IL11 and LIF regulate villous cytotrophoblast differentiation towards the invasive phenotype at least in part via CSPG4. PMID: 23953863
  24. NG2-Col VI interplay as putatively involved in the regulation of the cancer cell-host microenvironment interactions sustaining sarcoma progression PMID: 23559515
  25. This study showed that high CSPG4 expression correlates with disease recurrence and overall survival in breast cancers. PMID: 24177010
  26. Transgenic NG2-positive glia are identified as the major central nervous system cellular target of IL-17 in experimental autoimmune encephalomyelitis. PMID: 23995070
  27. tThis study demonistrated taht human infants, Western blot analyses exhibited trends for lower NG2 levels in the germinal matrix and white matter of infants with Intraventricular hemorrhage relative to controls without Intraventricular hemorrhage. PMID: 23474192
  28. cell polarity complexes as new effectors of NG2 signaling in the establishment of front-rear polarity PMID: 23804106
  29. NG2-positive adipose stem cells loaded on scaffolds fabricate skeletal muscle tissue in vivo without the need of a myogenic pre-differentiation step in vitro. PMID: 23359523
  30. NG2 mediates activation of Rho leading to amoeboid invasiveness in sarcoma cell line. PMID: 22699001
  31. Although transgenic nestin-GFP-expressing progenitor cells share morphological and molecular markers with NG2-glia, they do not express other pericyte markers nor do they differentiate into the muscle lineage. PMID: 22999866
  32. REVIEW outlines recent advances in our understanding of CSPG4-associated cell signaling, describing the central role it plays in melanoma tumor cell growth, motility, and survival, and explores how modifying CSPG4 function and protein-protein interactions may provide us with novel combinatorial therapies for the treatment of advanced melanoma PMID: 22004131
  33. Cell surface P-selectin binding depends on CHST11 gene expression. CSPG4 serves as a P-selectin ligand through its CS chain and participates in P-selectin binding to the highly metastatic breast cancer cells. PMID: 21658254
  34. Findings define the CSPG4-specific fully human scFv-FcC21 antibody as a candidate therapeutic agent to target the many types of tumors that express CSPG4. PMID: 22021902
  35. High NG2 expression is associated with glioblastoma. PMID: 21798846
  36. Functional and clinical relevance of chondroitin sulfate proteoglycan 4. PMID: 21070915
  37. In regions of spinal cord neurodegeneration of amyotrophic lateral sclerosis model mice, NG2-positive cells exhibit enhanced proliferation and accelerated differentiation into oligodendrocytes but remained committed to the oligodendrocyte lineage. PMID: 21092857
  38. Cells that express NG2 proteoglycan act predominantly as a reservoir of new oligodendrocytes in the demyelinated spinal cord. PMID: 21123584
  39. Results showed the expression of MCSP and PRAME in conjunctival melanoma and benign conjunctival nevi and showed that MCSP and PRAME were differentially expressed in both and can help to differentiate the lesions diagnostically. PMID: 20805128
  40. Staining intensity of HMW-MAA in acral lentiginous melanoma lesions was weaker than in superficial spreading melanoma where it was found to be higher than previously reported. PMID: 20448346
  41. in squamous cell carcinoma of head and neck and in basal breast carcinoma, CSPG4 is expressed by cancer stem cells PMID: 20455858
  42. Human skin aging is associated with reduced expression of the stem cell markers beta1 integrin and MCSP PMID: 19776755
  43. identified a novel repeat named CSPG in the central ectodomain PMID: 12220645
  44. Data suggest that MCSP is a novel marker for epidermal stem cells that contributes to their patterned distribution by promoting stem cell clustering. PMID: 14573520
  45. MCSP coexpression with a subset of beta 1 integrin basal keratinocytes within the epidermis suggests that MCSP colocalizes with epidermal stem cells, MSCP expression within the hair follicle was more complex PMID: 15009727
  46. MCSP may facilitate primary melanoma progression by enhancing the activation of key signaling pathways important for tumor invasion and growth. PMID: 15210734
  47. PKC-alpha-mediated NG2 phosphorylation at Thr(2256) is a key step for initiating cell polarization and motility PMID: 15504744
  48. MCSP/NG2 proteoglycan may be considered an important receptor mediating COL6-sarcolemma interactions, a relationship that is disrupted by the pathogenesis of UCMD muscle. PMID: 16169245
  49. tumour cells can regulate both the function and structure of the host-derived tumour vasculature through NG2 expression PMID: 16253523
  50. Co-expression and interaction of NG2 and galectin-3 in human glioma cells establish a molecular basis for the NG2/galectin-3 interaction. PMID: 16365873

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Subcellular Location Cell membrane; Single-pass type I membrane protein; Extracellular side. Apical cell membrane; Single-pass type I membrane protein; Extracellular side. Cell projection, lamellipodium membrane; Single-pass type I membrane protein; Extracellular side. Cell surface.
Tissue Specificity Detected only in malignant melanoma cells.
Database Links

HGNC: 2466

OMIM: 601172

KEGG: hsa:1464

STRING: 9606.ENSP00000312506

UniGene: Hs.513044

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