Code | CSB-EL006076HU |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human CSPG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 92 | ||||
Range % | 87-95 | |||||
1:2 | Average % | 89 | ||||
Range % | 83-94 | |||||
1:4 | Average % | 92 | ||||
Range % | 89-95 | |||||
1:8 | Average % | 97 | ||||
Range % | 92-103 |
The recovery of human CSPG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 96 | 91-102 | ||||
EDTA plasma (n=4) | 88 | 82-94 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Human CSPG4 ELISA Kit was designed for the quantitative measurement of Human CSPG4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.
Sample type: Cell culture supernatant
Sample species: Human
Sample dilution: No dilution
Review: I used CSB-EL006076HU ELISA kit to quantify Chondroitine Sulphate both after intestinal passage (on CaCo-2 cells) and after entering in the chondrocyte (T/C-28a2). This kit allowed to accurately measure the presence of chondroitin in our samples and for this reason I would suggest to buy it.
By Anonymous
Could you tell me which sequence of the protein is recognised by the antibody of your kit ?