Human Clara cell protein,CC16 ELISA Kit

Code CSB-E08680h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details


The Human Clara cell protein (CC16) ELISA Kit quantifies CC16 in human serum, plasma, or tissue homogenates. It is not intended for diagnostic use and only used for scientific research in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.

This assay is based on the Sandwich-ELISA mechanism (the CC16 in the sample is sandwiched between the pre-coated CC16 antibody and HRP-labeled CC16 antibody) and enzyme-substrate chromogenic reaction. The color intensity is positively related to the amount of CC16 bound in the initial step. The levels of CC16 in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

CC16 is a homodimeric protein abundantly secreted in airways by the non-ciliated bronchiolar Clara cells. CC16 deficient transgenic mice were associated with a rising susceptibility of the lung to viral infections and oxidative stress, which demonstrated the potential role of CC16 in the protection of the respiratory tract against oxidative stress and inflammation. In vitro, CC16 participates in regulating the production and the activity of various mediators of the inflammatory response including PLA2, interferon-ɤ, and TNF-α. CC16 has also been found to suppress fibroblast migration or to bind various endogenous or exogenous substances such as polychlorobiphenyls (PCBs).

Alternative Names Blastokinin ELISA Kit; CC10 ELISA Kit; CC16 ELISA Kit; CCPBP ELISA Kit; CCSP ELISA Kit; Clara cell phospholipid binding protein ELISA Kit; Clara cell phospholipid-binding protein ELISA Kit; Clara cell specific 10 kD protein ELISA Kit; Clara cells 10 kDa secretory protein ELISA Kit; OTTHUMP00000236107 ELISA Kit; SCGB1A1 ELISA Kit; Secretoglobin family 1A member 1 ELISA Kit; Secretoglobin, family 1A, member 1 (uteroglobin) ELISA Kit; UG ELISA Kit; UGB ELISA Kit; UP-1 ELISA Kit; UP1 ELISA Kit; Urinary protein 1 ELISA Kit; Urine protein 1 ELISA Kit; UTER_HUMAN ELISA Kit; Uteroglobin ELISA Kit
Abbreviation CC16
Uniprot No. P11684
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/ml - 40 ng/ml
Sensitivity 0.156 ng/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Materials provided
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CC16 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled CC16 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
Gene References into Functions
  1. There was no evidence that serum CC16 levels played a role in recurrent wheezing and a positive asthma predictive index in pre-school children PMID: 29685782
  2. Data suggest that the increase in plasma club cell protein (CC16) CC16 following inhaled allergen as a biomarker of epithelial dysfunction. PMID: 28862880
  3. We propose that increased CC-16 levels is a marker of lung inflammatory response that associated with ventilatory insufficiency are related to impending respiratory failure, not fully predicted by conventional respiratory tests PMID: 28967121
  4. Studied serum CC16 levels in The serum CC16 levels in Acute respiratory distress syndrome (ARDS) and non-ARDS patients; found CC16 to be were significantly higher in ARDS patients than that in non-ARDS patients. PMID: 28548310
  5. Studied serum CC16 levels in relation to atopy and previously manifested low respiratory tract diseases. Found low serum CC16 is associated with previously expressed pneumonia and chronic wheezing in atopic children. PMID: 28146340
  6. Our data suggest that rCC16 suppresses LPS-mediated inflammatory mediator TNF-alpha, IL-6, and IL-8 production by inactivating NF-kappaB and p38 MAPK but not AP-1 in RAW264.7 cells. PMID: 28338974
  7. There were associations between urinary CC16 and arsenic concentration in soil, water, house dust, and dust loading. In multiple analyses, only the concentration of arsenic in soil was associated with urinary CC16 levels after accounting for other factors. PMID: 27223295
  8. findings demonstrate that CC16 is upregulated in IPF patients suggesting that may participate in its pathogenesis. PMID: 27977812
  9. The G38A CCSP gene polymorphism may alter either the production of the protein and/or its activity in chronic obstructive pulmonary disease. PMID: 27496897
  10. CC16 levels differed among idiopathic pulmonary fibrosis, pulmonary sarcoidosis and chronic pulmonary obstructive disease. PMID: 27758987
  11. Lung-specific (CC-16) and novel (RelB) biomarkers are associated with systemic cardiovascular changes over time. CC-16 can predict subsequent exacerbations in subjects with severe COPD and may be an important biomarker of pulmonary and systemic stress in COPD. PMID: 26914709
  12. The serum concentration of CC16 was significantly higher in patients with lung fibrosis. PMID: 25244495
  13. lower levels of urine CC16 and lung function in patients with asthma than in those patients without asthma. CC16 in urine may be a useful tool or biomarker for investigating lung epithelium integrity among children with asthma or lung injury. PMID: 26108072
  14. Smokers and COPD patients had reduced airway CC16 immunostaining that decreased with increasing COPD severity. PMID: 25700379
  15. the uteroglobin G38A gene polymorphism was not associated with IgAN risk PMID: 25068828
  16. Sputum and bronchoalveolar lavage fluid CC16 were significantly higher in patients with severe asthma compared to mild-moderate asthma and healthy controls. PMID: 25728058
  17. In chromium-exposed workers, blood levels of CC16, and CC16/SP-D were lower than in controls. Positive relationships were shown between CC16 or CC16/SP-D and indicators of lung function. PMID: 25851191
  18. KL-6 levels were higher and CC16 levels were lower in infants with poor neurodevelopmental outcome compared with those infants who had favourable neurodevelopmental outcome. PMID: 25631862
  19. CC16 may play an important protective role in cigarette smoke-related diseases. PMID: 25635997
  20. A meta-analysis indicated that the CC16 gene A38G polymorphism is not associated with the risk of asthma. PMID: 25743006
  21. Increased plasma clara cell secretory protein levels are associated with primary graft dysfunction. PMID: 24400993
  22. Uteroglobin is a possible ligand of the lipoxin receptor and it may have a role in inhibiting serum amyloid A-driven inflammation PMID: 24782597
  23. Athletes with decreased serum CC16 from regular high-load exercise are more susceptible to respiratory infections. PMID: 24735334
  24. A single therapeutic dose of terbutaline offers significant protection against hyperpnoea-induced bronchoconstriction and reduces urinary CC16 protein levels. PMID: 24030662
  25. Lung permeability biomakers [surfactant protein D (SP-D) and Clara cell secretory protein (CC16) in plasma] and forced expiratory volumes and flow were measured in swimmers in indoor swimming pool waters treated with different disinfection methods. PMID: 23874631
  26. Serum CC-16 is associated with disease progression in chronic obstructive pulmonary disease (COPD). However, the absence of CC-16 does not appear to modify the risk of cigarette-related COPD in mice. PMID: 24245748
  27. Acute exposure to smoke induces injury at the alveolar level, which results in a transient increase of CC16 in serum of exposed subjects. PMID: 23258467
  28. These results suggest that the CC16 A38G polymorphism may play a role in asymptomatic airway hyper-responsiveness and contribute to the development of late-onset asthma. PMID: 24125144
  29. The SCGB1A1 +38A/G polymorphism is a risk factor for asthma. [Meta-analysis] PMID: 23820082
  30. urinary CC16 may be a useful biomarker of increased lung epithelial permeability among female non-smokers; further work will be required to evaluate its applicability to males PMID: 22805990
  31. Clara cell secretory protein expression changes is a relevant marker in the development of bronchiolitis obliterans patients with lung transplantation. PMID: 22883104
  32. Overview of CC16 in pathophysiology of and as a biomarker in chronic obstructive pulmonary disease. PMID: 23030587
  33. A Genome wide association study for COPD biomarkers on subjects with COPD found 2 discrete loci affecting CC16, one near the CC16 coding gene (SCGB1A1) on chromosome 11 and one approximately 25Mb away from SCGB1A1, identified in expressed sputum. PMID: 23144326
  34. Donor CCSP A38G polymorphism is associated with decreased CCSP levels early after lung transplantation and poor long-term outcomes. PMID: 22902791
  35. Exercise caused an increase in urinary excretion of CC16 in all subjects (P < 0.001), but this rise in CC16 was blunted following inhalation of warm humid air. PMID: 21799131
  36. demonstration that cc-10 is differentially expressed in infants with iRDS may point the way towards one possible mechanism that potentially involves modifications of the protein structure with its anti-inflammatory and surfactant protective function PMID: 22613976
  37. CC10 G+38A variant may contribute to the severity of asthma and lower level of steroid responsiveness. PMID: 22788242
  38. These results indicate that CC10 gene transfer may inhibit airway inflammation through suppressing the activation of NF-kappaB. PMID: 22558282
  39. Serum Clara cell secretory protein levels were characterized by an early postnatal surge. This apparent gestation-influenced surge may represent an initiation of a protective cascade against postnatal lung injury during extrauterine adaptation. PMID: 21952535
  40. Single-nucleotide polymorphism in the CC10 gene (A38G) does not seem to be involved in the severity of respiratory syncytial virus infection or wheezing. PMID: 21767304
  41. Urinary levels of CC16 are increased after eucapnic voluntary hyperpnea. PMID: 21131866
  42. Elevated circulating CC16 levels identified severe thoracic injury combined with a strong correlation with the extent/volume of affected lung tissue. PMID: 21045740
  43. CC16 38A/38A genotype plays a role in the development of early asthma in children with AR. PMID: 21255142
  44. Elevated plasma clara cell secretory protein concentration is associated with high-grade primary graft dysfunction. PMID: 21299834
  45. In the context of allergic airway responses, CC10 can inhibit OPN expression and suppress the Th2-promoting function of OPN, resulting in CC10's inhibitory biological effects. PMID: 20553297
  46. Data suggest that a supernatant of non-small-cell lung cancer causes an imbalance in the immune response of PBMCs and DCs, which is reversed by CC-10. PMID: 20664959
  47. Letter: serum CCSP cannot be used as a biomarker predictive for bronchiolitis obliterans after lung transplantation. PMID: 20683434
  48. Reduced anti-inflammatory CC10 concentrations in airways of extremely premature infants with a fetal inflammatory response, not umbilical cord serum CC10, might make their lungs susceptible for further postnatal injuries. PMID: 19887851
  49. Association of CC16 with daily outdoor particulate matter from combustion sources increases epithelial barrier permeability in lungs. PMID: 19852548
  50. The effect of G38A may be apparent under stimulation as sex steroids or infections, and homozygotes of the G38A mutation cannot produce sufficient UG in response to stimulation and may be predisposed to IgA nephropathy, especially in childhood. PMID: 11774099

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Subcellular Location Secreted.
Protein Families Secretoglobin family
Tissue Specificity Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
Database Links

HGNC: 12523

OMIM: 192020

KEGG: hsa:7356

STRING: 9606.ENSP00000278282

UniGene: Hs.523732

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