Human Claudin-2(CLDN2) ELISA kit

Code CSB-EL005500HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human CLDN2 ELISA Kit was designed for the quantitative measurement of Human CLDN2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

Target Name claudin 2
Alternative Names CLDN2; PSEC0059; SP82; UNQ705/PRO1356; Claudin-2; SP82
Abbreviation CLDN2
Uniprot No. P57739
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %84
Range %80-92
1:2Average %90
Range %85-95
1:4Average %97
Range %92-103
1:8Average %93
Range %88-98
Recovery
The recovery of human CLDN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 104100-109
EDTA plasma (n=4)9690-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.671 2.552 2.612 2.449
7502.124 2.006 2.065 1.902
3751.438 1.403 1.421 1.258
187.50.806 0.839 0.823 0.660
940.508 0.524 0.516 0.353
470.294 0.309 0.302 0.139
23.50.234 0.227 0.231 0.068
00.161 0.164 0.163
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Gene References into Functions
  1. CLDN2 may have a role regulating tissue-specific physiological properties in tight junctions in UC. PMID: 29040430
  2. the accumulation of DXR into spheroids was enhanced by claudin-2 knockdown, resulting in a reduction in cell viability. We suggest that claudin-2 may be a novel therapeutic target in lung adenocarcinoma, because claudin-2 knockdown increased the accumulation of anticancer agents in cancer cells and spheroids. PMID: 29247669
  3. integration of claudin-2, occludin and ZO-1 is necessary for maintaining the function of the proximal tubular epithelium. PMID: 29252987
  4. these findings provide a novel mechanism by which microbial-derived butyrate promotes barrier through IL-10RA-dependent repression of claudin-2 PMID: 28893958
  5. Low CLDN2 expression is associated with Lung Cancer metastasis. PMID: 28754672
  6. results reveal that IL-22 increases intestinal epithelial permeability by upregulating Claudin-2 expression through the JAK/STAT pathway PMID: 28939759
  7. In summary, we demonstrate that TCPTP protects the intestinal epithelial barrier by restricting STAT-induced claudin-2 expression. PMID: 28804910
  8. The cation channel-forming tight junction protein claudin-2 was shown to permit the paracellular passage of water through its pore. PMID: 28636801
  9. suggest that epigenetic inhibitors suppress the abnormal proliferation of lung adenocarcinoma cells highly expressing claudin-2 PMID: 28057758
  10. Data show that alterations in myosin light chain kinase activity, claudin-15 and claudin-2 expression are associated with gluten-induced symptomatology and intestinal permeability changes in diarrhea-predominant irritable bowel syndrome (IBS-D). PMID: 27869798
  11. hypotonic stress induces dephosphorylation, clathrin-dependent endocytosis, and degradation of claudin-1 and -2 in lysosomes, resulting in disruption of the TJ barrier in renal tubular epithelial cells. PMID: 27733684
  12. The claudin-2 expression increased in tumor necrosis factor alpha (TNF-alpha) Treated Caco-2 cell monolayers. PMID: 28106723
  13. The authors conclude that claudin-2 forms gated paracellular channels. PMID: 26568313
  14. Association of claudin2 (rs7057398) polymorphism with idiopathic recurrent acute pancreatitis and progression of the disease to chronic pancreatitis. PMID: 26110235
  15. Genetic variants in CLDN2 and MORC4 genes were associated with chronic pancreatitis in Indian patients. PMID: 26820620
  16. Quercetin decreases claudin-2 expression mediated by up-regulation of miR-16 expression and instability of claudin-2 mRNA in lung adenocarcinoma cells. PMID: 26061016
  17. Pan-inhibition of SFK signaling pathways elevated Claudin-2 expression levels in breast cancer cells. Loss of Yes or Fyn induces Claudin-2 expression; whereas, diminished Lyn levels impairs Claudin-2 expression in breast cancer cells. PMID: 25823815
  18. PKCiota binds to Rab14 and that PKCiota requires Rab14 for its correct distribution in cells. As with Rab14, PKCiota protects claudin-2 from lysosomal degradation and, in consequence, modulates epithelial barrier. PMID: 25694446
  19. Phosphorylation state of the tyrosine of claudin-1 and claudin-2 regulates interaction with ZO1. PMID: 26023235
  20. Single nucleotide polymorphism is associated with the development of alcoholic pancreatitis. PMID: 25253127
  21. TNF--alpha modifies claudin-2 expression in tubular cells and this can have both short-term and long-term effects on transport and barrier functions. PMID: 25948735
  22. Claudin-5 overexpression promoted the interactions of claudin-1 and claudin-2 and enhanced the barrier function of retinal cells. PMID: 25323998
  23. Claudins 2 and 4 have reciprocal effects on epithelial barrier function, exhibit differential FRAP dynamics, and compete for residency within the tight junction. PMID: 25031428
  24. Autophagy reduces the paracellular permeability of small solutes and ions via degradation of the pore-forming tight junction protein claudin-2. PMID: 25616664
  25. Decreased claudin-2 enhances endothelial barrier function and may play a role in the prolonged survival of patients with Kaposi sarcoma-associated herpesvirus and HIV co-infection. PMID: 24995964
  26. IL-6 modulation of intestinal TJ permeability was regulated by JNK activation of AP-1 and AP-1 activation of claudin-2 gene PMID: 24662742
  27. Suggest that claudin 2 is involved in paracellular water transport and that claudin 2 expression is sensitive to compound induced cellular stress. PMID: 24907557
  28. The expression of claudin-2 was down regulated in gastric cancer tissue. PMID: 23919729
  29. In breast cancer patients CLDN2 was significantly up-regulated (P < 0.001) in liver metastases compared to other metastatic sites. PMID: 24287398
  30. Proliferation of human adenocarcinoma A549 cells was decreased by claudin-2 knockdown together with a decrease in the percentage of S phase cells. PMID: 24907662
  31. Report altered claudin 1 expression when comparing normal skin to actinic keratosis and squamous cell carcinoma of the skin. PMID: 24294371
  32. Helicobacter pylori-induced tumorigenesis and invasiveness of gastric carcinoma cells is mediated by bacterial CagA via Cdx2-dependent targeting of Claudin-2. PMID: 24287273
  33. Findings suggest that a variant of the CLDN2-MORC4 region predisposes to Crohn's disease in a Swedish population. PMID: 23946598
  34. Claudin-2 protein expression is elevated in intestinal epithelia, in response to bovine colostrum, without altering ion permeability. PMID: 23717570
  35. generated MDCK I Tet-off cells stably transfected with claudin-2 Tyr(67) mutants. The Y67L mutant showed reduced cation selectivity compared with wild-type claudin-2 due to a decrease in Na(+) permeability, without affecting the Cl(-) permeability PMID: 23760508
  36. Claudin-2 assumes an important role in colorectal inflammation, and furthermore implicates the involvement of MLCK in colon inflammation. PMID: 23306855
  37. Sinonasal epithelium in allergic fungal rhinosinusitis displays increased epithelial permeability and an altered expression of claudin-2 PMID: 22927233
  38. results implicate pericryptal myofibroblast-derived paracrine KGF and largely autocrine amphiregulin in the upregulation of claudin-2 in Caco-2 epithelial monolayers and consequent disruption of tight junction integrity PMID: 22946653
  39. Two associations with alcoholic pancreatitis at genome-wide significance were identified and replicated at PRSS1-PRSS2 and X-linked CLDN2 through a two-stage genome-wide study. PMID: 23143602
  40. These results suggest that EGF is secreted from A549 cells by MMP and increases claudin-2 expression mediated via the activation of an EGFR/MEK/ERK pathway. PMID: 22546605
  41. Pore-forming tight junction protein claudin-2 is strongly expressed in the ileum of 51% patients in quiescent phase and in 49% of the patients with active Crohn's disease. PMID: 21688348
  42. demonstrate here that breast cancer cells harbouring PIK3CA mutations are selectively sensitive to mTOR allosteric and kinase inhibitors. However, cells with PTEN loss of function are not sensitive to these drugs PMID: 21383692
  43. CLDN2 is selectively enriched in and promotes the formation of breast cancer liver metastases through engagement of cell membrane complexes. PMID: 21076473
  44. The authors propose claudin-2 and SYN1 work in concert to enhance microbial translocation across the intestinal epithelial barrier to contribute to chronic immune activation and CD4 T-cell depletion in HIV-1-infected patients. PMID: 20700059
  45. claudin-2 was not expressed in any of the 18 cases of Epstein-Barr virus-associated nasopharyngeal carcinoma studied. PMID: 20204275
  46. CLDN 2 expression was significantly increased in severe form of coeliac disease in bulb and in distal duodenum PMID: 20143085
  47. Cloning of the human claudin-2 5'-flanking region revealed a TATA-less promoter with conserved binding sites in mouse and human for caudal-related homeodomain proteins and hepatocyte nuclear factor-1alpha. PMID: 11934881
  48. Results support a model in which claudins 2 and 4 create paracellular channels and the first extracellular domain is sufficient to determine both paracellular charge selectivity and transepithelial electrical resistance. PMID: 12700140
  49. role for the paracellular barrier function by opening pores for small cations PMID: 14751232
  50. optimal claudin-2 expression in the gut relies on the presence of GATA-4, suggesting a role for this factor in intestinal regionalization PMID: 15389642

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Subcellular Location Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
Protein Families Claudin family
Database Links

HGNC: 2041

OMIM: 300520

KEGG: hsa:9075

STRING: 9606.ENSP00000336571

UniGene: Hs.522746

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