Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT

This ELISA kit provides quantitative measurement of Collagen Type III (Col III) in human serum, plasma, and tissue homogenates, supporting research into extracellular matrix remodeling and fibrotic signaling pathways. Because Col III turnover fragments and propeptides fluctuate across stages of tissue fibrosis, wound healing, and vascular remodeling, researchers need an assay calibrated to capture both baseline and disease-elevated concentrations across diverse sample matrices.

Detection Range: The 4.69–300 ng/mL range spans the physiological concentrations of circulating Col III-related fragments reported in healthy individuals through the markedly elevated levels seen in hepatic fibrosis, systemic sclerosis, and post-surgical wound repair, minimizing the need for extensive sample dilution in most serum and plasma applications.

Sensitivity: At 2.345 ng/mL, the assay detects Col III at concentrations relevant to early-stage fibrotic processes and low-turnover conditions, enabling researchers to identify subtle changes before overt tissue remodeling is established.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates allows direct comparison between circulating Col III fragments and local deposition within fibrotic organs such as liver, lung, or skin biopsies within a single experimental framework.

Assay Efficiency: A 1–5 hour protocol requiring only 50–100 µL per well accommodates studies using limited clinical biopsy homogenates or serial blood draws from longitudinal fibrosis monitoring cohorts.

This kit is particularly suited for investigating hepatic fibrosis staging, dermal wound healing progression, and cardiovascular extracellular matrix remodeling.