Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit

Code CSB-E08082h
Size 96T,5×96T,10×96T
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Product Details

Target Name
Collagen Type Ⅰ,Col Ⅰ
Associated names
Collagen Type 1,Col 1,Collagen Type I,Col I
Abbreviation
Col Ⅰ
Species
Homo sapiens (Human)
Sample Types
serum, urine, cell culture supernates, tissue homogenates
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 80-92  
1:2 Average % 100  
Range % 91-110  
1:4 Average % 105  
Range % 92-110  
1:8 Average % 93  
Range % 86-100  
Recovery
The recovery of human Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.921 2.985 2.953 2.767  
50 2.356 2.378 2.367 2.181  
25 1.826 1.875 1.851 1.665  
12.5 1.221 1.256 1.239 1.053  
6.25 0.796 0.800 0.798 0.612  
3.12 0.512 0.546 0.529 0.343  
1.56 0.302 0.326 0.314 0.128  
0 0.188 0.184 0.186    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Human Col Ⅰ ELISA Kit was designed for the quantitative measurement of Human Col Ⅰ protein in serum, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 11 reviews

Submit a Review here

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: 1:5

Review: The collagen 1 content in cells was detected using the CSB-E08082h kit with a correct trend of the calibration curve and intact data.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: No dilution

Review: CSB-E08082h was used to detect exosomes promoting collagen type I production in fibroblasts, and its OD value is shown in the attached graph. The instruction manual is very detailed in the process of using, and it is very smooth to do the experiment according to the steps.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: 1:20

Review: I used CSB-E08082h product to detect collagen type I in the cell supernatant, and the standard curve was normal... The final color development time of the product was extended to 30min, OD ranged from 0.1 to 0.3, and the COL I kit was used in the experiment, and the samples were diluted 20-to 50-fold.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: : 1:15

Review: I used CSB--E08082h to detect cell supernatant. The OD values of the standard curve ranged from 0.0902 to 2.61, and the OD values of the samples were in the range of 0.300-0.4400. The standard curve fitting value R2 was 0.99847, indicating a good fitting effect.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: : 1:9

Review: I used the Human collagen type I ELISA Kit to test the content of type I collagen. The sensitivity (minimum detection limit) of this kit can reach 1.56 pg/mL, which can accurately detect trace target proteins in samples. This kit is suitable for various common biological samples such as serum, plasma, cell culture supernatant, and tissue homogenate, with a wide range of applications. The experimental test is convenient, and the entire detection process takes about 4-5 hours.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: : 1:5

Review: I used the CSB-E08082h kit to test cell culture supernatants. The standard curve data fitting value r²=0.99908, which meets the expected effect. There are significant differences in OD values between different groups. The operation process is simple and easy to understand.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: : 1:5

Review: We used the CSB-E08082h kit to detect normal cell supernatants. The standard curve was between 0-3, with good standard linearity meeting the requirements. The sample OD values were all within the curve range, meeting the requirements. The kit has high precision, high accuracy, and is convenient and efficient.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: : 1:5

Review: I used the CSB-E08082h kit to detect fibroblasts. The sample was diluted 5 times, and the sample OD was between 0.2 and 0.5, while the standard curve OD was between 0.07 and 2.2. The linearity is good and the detection results are accurate.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: : 1:5

Review: We used CSB E08082h to detect the supernatant of normal cells, and the OD value was within the normal range. The OD of the standard area was between 0.07 and 3.0, with good linearity, small error, accurate detection results, good repeatability, the sample detection trend was in line with the detection requirements, and high precision.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: : 1:5

Review: We used CSB to detect normal cell supernatants. The OD values were within the range of the standard curve, and the sample trend also met the requirements. The OD values of the standard curve were between 0.08 and 2.5, with good linearity. The detection results had small errors and high precision, meeting the requirements.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: No dilution

Review: I used the CSB-608082h to detect cell supernatant samples. In the experiment, the OD value fitting degree of the standard curve was excellent, with R² reaching 0.9995, which conformed to the equation y=0.0087x+0.0488. The OD value range of actual samples was stable between 0.3 and 0.7, and the detection results were accurate and reproducible. The product is easy to operate, reliable in data, and an efficient and practical detection tool in experiments.

By Anonymous

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