Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit

Code CSB-E08082h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation Col Ⅰ
Target Name Collagen Type Ⅰ,Col Ⅰ
Alias Collagen Type 1,Col 1,Collagen Type I,Col I
Species Homo sapiens (Human)
Sample Types serum, urine, cell culture supernates, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 80-92  
1:2 Average % 100  
Range % 91-110  
1:4 Average % 105  
Range % 92-110  
1:8 Average % 93  
Range % 86-100  
Recovery
The recovery of human Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.921 2.985 2.953 2.767  
50 2.356 2.378 2.367 2.181  
25 1.826 1.875 1.851 1.665  
12.5 1.221 1.256 1.239 1.053  
6.25 0.796 0.800 0.798 0.612  
3.12 0.512 0.546 0.529 0.343  
1.56 0.302 0.326 0.314 0.128  
0 0.188 0.184 0.186    
References
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