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Human Collagen Type Ⅱ,Col Ⅱ ELISA Kit

Instructions
Code CSB-E08087h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Collagen Type Ⅱ,Col Ⅱ
Alternative Names Collagen Type 2,Col 2,Collagen Type II,Col II
Abbreviation Col Ⅱ
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:100 Average % 86  
Range % 80-90  
1:200 Average % 93  
Range % 88-98  
1:400 Average % 94  
Range % 90-100  
1:800 Average % 96  
Range % 90-102  
Recovery
The recovery of human Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-98  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.478 2.387 2.433 2.341  
1000 1.811 1.752 1.782 1.690  
500 1.237 1.142 1.190 1.098  
250 0.863 0.813 0.838 0.746  
125 0.398 0.374 0.386 0.294  
62.5 0.211 0.198 0.205 0.113  
31.25 0.145 0.141 0.143 0.051  
0 0.093 0.090 0.092 　  
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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