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| Code | CSB-EL006162HU |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTHRC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-96 | |||||
| The recovery of human CTHRC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 92-104 | ||||
| EDTA plasma (n=4) | 92 | 89-98 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human collagen triple helix repeat-containing protein 1 (CTHRC1) ELISA Kit is used to quantitatively measure human CTHRC1 concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of CTHRC1 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
CTHRC1 is a secreted extracellular matrix (ECM) protein transiently expressed in the repair process after arterial injury and myocardial infarction, thus regulating collagen matrix deposition and cell migration and promoting tissue repair through the TGF-β signaling pathway. It is also involved in some pathophysiological processes, including increasing bone mass, preventing myelination, and reversing collagen synthesis in many tumor cells. Increased expression of CTHRC1 is associated with tumorigenesis, proliferation, invasion, and metastasis in various types of human cancer, including gastric cancer, pancreatic cancer, breast cancer, colorectal cancer, non-small-cell lung carcinoma, and melanoma.
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