Human Complement component C8 alpha chain(C8A) ELISA kit

Instructions
Code CSB-EL004194HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name complement component 8, alpha polypeptide
Alternative Names C8A ELISA Kit; CO8A_HUMAN ELISA Kit; Complement component 8 subunit alpha ELISA Kit; Complement component 8, alpha polypeptide ELISA Kit; Complement component C8 alpha chain ELISA Kit
Abbreviation C8A
Uniprot No. P07357
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C8A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 85
Range % 80-89
1:2000 Average % 94
Range % 90-98
1:4000 Average % 96
Range % 92-100
1:8000 Average % 89
Range % 84-93
Recovery
The recovery of human C8A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 98 94-102
EDTA plasma (n=4) 104 100-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
100 2.563 2.472 2.518 2.373
50 2.272 2.169 2.221 2.076
25 1.729 1.684 1.707 1.562
12.5 1.253 1.293 1.273 1.128
6.25 0.785 0.801 0.793 0.648
3.12 0.464 0.462 0.463 0.318
1.56 0.316 0.320 0.318 0.173
0 0.141 0.148 0.145  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself.
Gene References into Functions
  1. C8 alpha and C8 beta have correspondingly similar roles in MAC-mediated lysis of erythrocytes and bacterial killing. C8 gamma is not required for complement-mediated killing of Gram-negative bacteria. PMID: 12413696
  2. Results describe the crystal structure of the human C8 alpha MACPF domain disulfide-linked to C8 gamma (alphaMACPF-gamma) at 2.15 A resolution. PMID: 18440555
  3. The binding specificity between C8 alpha and C8 beta subunits is determined by a cooperative interaction of the N-terminal thrombospondin type 1 module and the membrane attack complex/perforin domain. PMID: 12220191
  4. Both N-terminal modules in C8 alpha have a role in forming the principal binding site for C9, and binding may be dependent on a cooperative interaction between these modules and the C8 alpha membrane attack complex/perforin domain. PMID: 12463754
  5. result suggests that this segment of C8alpha and corresponding segments of the other MAC family members are independently folded domains PMID: 16618117
  6. One can predict that the indel segment of C8alpha assumes a conformation that allows for multiple points of contact with C8gamma. PMID: 16935577
  7. crystal structure of the MACPF domain of complement component C8alpha at 2.5 angstrom resolution was determined and it was shown that it is structurally homologous to the bacterial, pore-forming, cholesterol-dependent cytolysins PMID: 17872444
  8. C8gamma binds an indel peptide of C8alpha sequence and forms a non-covalent complex. PMID: 19048311

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Involvement in disease Complement component 8 deficiency, 1 (C8D1)
Subcellular Location Secreted, Cell membrane, Multi-pass membrane protein
Protein Families Complement C6/C7/C8/C9 family
Database Links

HGNC: 1352

OMIM: 120950

KEGG: hsa:731

STRING: 9606.ENSP00000354458

UniGene: Hs.93210

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