Human Complement component C8 beta chain(C8B) ELISA kit

Code CSB-EL004195HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name complement component 8, beta polypeptide
Alternative Names C8b ELISA Kit; CO8B_HUMAN ELISA Kit; Complement component 8 beta polypeptide ELISA Kit; Complement component 8 subunit beta ELISA Kit; Complement component C8 beta chain ELISA Kit
Abbreviation C8B
Uniprot No. P07358
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 2.74 pg/mL-2000 pg/mL
Sensitivity 1.37 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human C8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:10000 Average % 92
Range % 89-98
1:20000 Average % 90
Range % 84-100
1:40000 Average % 95
Range % 92-101
1:80000 Average % 93
Range % 86-98
The recovery of human C8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 85-104
EDTA plasma (n=4) 91 88-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.556 2.510 2.533 2.442
667 1.881 1.816 1.849 1.758
222 1.253 1.301 1.277 1.186
74 0.908 0.896 0.902 0.811
24.7 0.374 0.366 0.370 0.279
8.2 0.205 0.195 0.200 0.109
2.74 0.141 0.134 0.138 0.047
0 0.089 0.093 0.091  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
Gene References into Functions
  1. The binding specificity between C8 beta and C8 alpha subunits is determined by a cooperative interaction of the N-terminal thrombospondin type 1 module and the membrane attack complex/perforin domain. PMID: 12220191
  2. results indicate that the principal binding site for C9 lies within the MACPF domain of C8alpha; they also suggest this site and the binding sites for C8beta and C8gamma are distinct PMID: 16618117
Involvement in disease Complement component 8 deficiency, 2 (C8D2)
Subcellular Location Secreted
Protein Families Complement C6/C7/C8/C9 family
Database Links

HGNC: 1353

OMIM: 120960

KEGG: hsa:732

STRING: 9606.ENSP00000360281

UniGene: Hs.391835


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