Human Complement fragment 3c,C3c ELISA Kit

Code CSB-E11168h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Complement fragment 3c,C3c
Abbreviation
C3c
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, urine, saliva
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C3c in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5000Average %88
Range %84-92
1:10000Average %94
Range %90-98
1:20000Average %99
Range %95-104
1:40000Average %102
Range %96-108
Recovery
The recovery of human C3c spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8993-96
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.266 2.345 2.306 2.174
201.946 1.973 1.960 1.828
101.510 1.546 1.528 1.396
51.185 1.198 1.192 1.060
2.50.854 0.814 0.834 0.702
1.250.521 0.501 0.511 0.379
0.6250.335 0.325 0.330 0.198
00.131 0.133 0.132  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human C3c ELISA Kit was designed for the quantitative measurement of Human C3c protein in serum, plasma, cell culture supernates, urine, saliva. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

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