Human Core Binding Factor alpha1 CBFA1/RUNX2 ELISA Kit

Code CSB-E12935h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q13950
Lead Time 3-5 working days
Abbreviation RUNX2
Protein Biological Process 1 Transcription/Transcription regulation
Target Name runt-related transcription factor 2
Alias RP1-166H4.1, AML3, CBFA1, CCD, CCD1, MGC120022, MGC120023, OSF2, PEA2aA, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, CBF-alpha 1|SL3-3 enhancer factor 1 alpha A subunit|SL3/AKV core-binding factor alpha A
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.
HGNC 10472
RGD 2282
MGI 99829
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CBFA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:200 Average % 91    
Range % 86-95    
1:400 Average % 102    
Range % 97-107    
1:800 Average % 91    
Range % 85-97    
1:1600 Average % 97    
Range % 91-103    
Recovery
The recovery of human CBFA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 89-98    
EDTA plasma (n=4) 97 90-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
2000 1.957 1.999 1.978 1.887    
1000 1.567 1.603 1.585 1.494    
500 1.106 1.101 1.104 1.013    
250 0.719 0.732 0.726 0.635    
125 0.407 0.412 0.410 0.319    
62.5 0.283 0.298 0.291 0.200    
31.25 0.164 0.165 0.165 0.074    
0 0.090 0.092 0.091      
References
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Function Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis
Involvement in disease Cleidocranial dysplasia (CLCD); Metaphyseal dysplasia with maxillary hypoplasia with or without brachydactyly (MDMHB)
Subcellular Location Nucleus
Tissue Specificity Specifically expressed in osteoblasts.
Database Links

HGNC: 10472

OMIM: 119600

KEGG: hsa:860

STRING: 9606.ENSP00000360493

UniGene: Hs.535845

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