Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit

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Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit

Name: Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit
Brand: CUSABIO
SKU: CSB-E11224h

Citations (18) Protocol MSDS

Code	CSB-E11224h
Size	96T,5×96T,10×96T
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Product Details
Citations (18)
Customer Reviews and Q&A

Target Name

Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I

Alternative Names

Cross-linked Carboxy-terminal telopeptide of type 1 collagen, CTX-1,Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I

Abbreviation

CTX-I

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-95
1:2	Average %	95
	Range %	91-98
1:4	Average %	97
	Range %	90-103
1:8	Average %	96
	Range %	91-101

Recovery

The recovery of human CTX-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-92
EDTA plasma (n=4)	96	93-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.995	2.960	2.978	2.838
20	2.557	2.570	2.564	2.424
10	1.666	1.765	1.716	1.576
5	0.839	0.886	0.863	0.723
2.5	0.491	0.524	0.508	0.368
1.25	0.263	0.251	0.257	0.117
0.625	0.201	0.212	0.207	0.067
0	0.138	0.142	0.140

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human CTX-I ELISA Kit was designed for the quantitative measurement of Human CTX-I protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

Effects of metformin and Denosumab on bone and glucose metabolism in postmenopausal women with OP and OA X Jin, Z Wang, BG Zhang, J Yu, Z Guan,Journal of Endocrinology,2025
GPR37 Activation Alleviates Bone Cancer Pain via the Inhibition of Osteoclastogenesis and Neuronal Hyperexcitability K Wang, Y Zhang, R Shu, L Yuan, H Tu,Advanced Science,2025
C-terminal cross-linking telopeptide levels in COVID-19 patients: A prospective case-control study MA Gul,Advances in clinical and experimental medicine,2024
ERK-estrogen receptor α signaling plays a role in the process of bone marrow mesenchymal stem cell-derived exosomes protecting against ovariectomy-induced bone loss H Qi,Journal of orthopaedic surgery and research,2023
Effect of polyhydroxy-terminated PAMAM dendrimer on dentin matrix metalloproteinases within the hybrid layers Y Luo,BMC oral health,2023
Rosiglitazone Mitigates Dexamethasone-Induced Depression in Mice via Modulating Brain Glucose Metabolism and AMPK/mTOR Signaling Pathway A Alhaddad,Biomedicines,2023
Changes in bone turnover markers in patients without bone metastases receiving immune checkpoint inhibitors: An exploratory analysis F Pantano,Journal of bone oncology,2022
Praeruptorin B inhibits osteoclastogenesis by targeting GSTP1 and impacting on the S-glutathionylation of IKKβ K Xu,Biomedicine & pharmacotherapy,2022
TAZ inhibits osteoclastogenesis by attenuating TAK1/NF-κB signaling W Yang,Bone Research,2021
Umbilical cord N-terminal procollagen of type l collagen (P1NP) and beta C-terminal telopeptide (βCTX) levels in term pregnancies with vitamin D deficiency M Eraslan Sahin,Journal Gynecological Endocrinology,2020
Efficacy and Safety of Kudzu Flower–Mandarin Peel on Hot Flashes and Bone Markers in Women during the Menopausal Transition: A Randomized Controlled Trial JE Kim,Nutrients,2020
Association between bile acid metabolism and bone mineral density in postmenopausal women Zhao YX,Clinics (Sao Paulo),2020
The inhibitory effect of carboxyl-terminated polyamidoamine dendrimers on dentine host-derived matrix metalloproteinases in vitro in an etch-and-rinse adhesive system Wu Q, et al,Royal Society Open Science,2019
The Circadian Gene Clock Regulates Bone Formation Via PDIA3 Yuan G.et al,J Bone Miner Res. ,2016
RANKL/Osteoprotegerin System and Bone Turnover in Hashimoto Thyroiditis. Konca Degertekin C.et al,Calcif Tissue Int.,2016
Effect of Vitamin D on Peripheral Blood Mononuclear Cells from Patients with Psoriasis Vulgaris and Psoriatic Arthritis Cubillos S.et al,PLoS One.,2016
Combination Treatment with Whole Body Vibration and a Kidney-tonifying Herbal Fufang Prevent Osteoporosis in Ovariectomized Rats Wei QS et al,Orthop Surg,2015
Alteration of Cartilage Degeneration and InflammationMarkers in Temporomandibular Joint Osteoarthritis Occurs Proportionally Vos LM et al,J Oral Maxillofac Surg,2013

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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■ Q&A

Please provide the following information regarding Human Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I ELISA Kit CSB-E11224h
a. Is the capture antibody a monoclonal or polyclonal?
b. What is the host of the capture antibody?
c. Is the detection antibody a monoclonal or polyclonal?
d. What is the host of the detection antibody?
e. B208. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
f. If recombinant protein, is it full length or partial?
g. What is the species of the standard– human or something else?

Thanks for your inquiry.
CSB-E11224h
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is synthetic peptide.
Pls let me know if you have any further questions. Thank you.

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