Human Cystatin-SN(CST1) ELISA kit

Instructions
Code CSB-EL006088HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Alternative Names CST1 ELISA Kit; Cystain SAI ELISA Kit; Cystain-SA-I ELISA Kit; Cystatin 1 ELISA Kit; Cystatin-1 ELISA Kit; Cystatin-SN ELISA Kit; Cystatin1 ELISA Kit; Cysteine proteinase inhibitor, type 2 family ELISA Kit; CYTN ELISA Kit; CYTN_HUMAN ELISA Kit; Salivary cystatin SA1 ELISA Kit; Salivary cystatin-SA-1 ELISA Kit
Abbreviation CST1
Uniprot No. P01037
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates,urine, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CST1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 92-97  
1:2 Average % 100  
Range % 98-104  
1:4 Average % 97  
Range % 92-101  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of human CST1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-100  
EDTA plasma (n=4) 93 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.406 2.389 2.398 2.278  
5 1.841 1.776 1.809 1.689  
2.5 1.246 1.223 1.235 1.115  
1.25 0.695 0.685 0.690 0.570  
0.625 0.464 0.444 0.454 0.334  
0.312 0.252 0.249 0.251 0.131  
0.156 0.187 0.185 0.186 0.066  
0 0.122 0.118 0.120    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.
Gene References into Functions
  1. Results revealed that CST1 expression was upregulated in colon cancer (CC) compared with normal tissues and contributed to CC cell proliferation. PMID: 29845224
  2. High CST1 expression is negatively correlated with survival of breast cancer patients. CST1 promotes cell proliferation, clone formation, and metastasis in breast cancer cells. CST1 is a novel potential prognostic biomarker and therapeutic target for breast cancer. PMID: 28523467
  3. this results identified cystatin 1 as a biomarker in patients with seasonal allergic rhinitis PMID: 28633877
  4. These results indicate that CST1-mediated extracellular CatB activity enhances tumor development by preventing cellular senescence. PMID: 28383558
  5. interactions of human family 1 & 2 cystatins with cathepsin L1 PMID: 27764212
  6. high expression of Cystatin SN is a significant prognostic indicator of a higher rate of recurrence, metastatic risk, and poor survival in patients with surgically resected NSCLCs. PMID: 25648368
  7. In conclusion, our data suggest that CST1 might contribute to the proliferation of pancreatic cancer cells and could be a potential biomarker for the early detection of pancreatic cancer. PMID: 25577248
  8. CST1 may contribute to inactivation of protease allergens and help re-establish homeostasis of the nasal membranes. PMID: 23950865
  9. CST1 expression at both the messenger RNA and protein levels was barely detected in control cells, which included early passage proliferating, quiescent, or immortal human fibroblasts PMID: 21636832
  10. Murine monoclonal antibodies were made which can distinguish between CST1 and CST2. PMID: 15829315
  11. CST1 might be highly involved in gastric tumorigenesis and regulate the proteolytic activity of cysteine proteases. PMID: 19463800
  12. Identification of Cystatin SN as a novel tumor marker for colorectal cancer. PMID: 19513549

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Subcellular Location Secreted
Protein Families Cystatin family
Tissue Specificity Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid.
Database Links

HGNC: 2473

OMIM: 123855

KEGG: hsa:1469

STRING: 9606.ENSP00000305731

UniGene: Hs.123114

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