Human Cystatin-SN(CST1) ELISA kit

Code CSB-EL006088HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01037
Lead Time 7-14 working days
Abbreviation CST1
Target Name cystatin SN
Alias , OTTHUMP00000164184|cystatin 1|cystatin SA-I|cysteine proteinase inhibitor, type 2 family
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, saliva, urine, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid.
HGNC 17447
RGD 1306458
MGI 1929895
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CST1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 92-97  
1:2 Average % 100  
Range % 98-104  
1:4 Average % 97  
Range % 92-101  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of human CST1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-100  
EDTA plasma (n=4) 93 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.406 2.389 2.398 2.278  
5 1.841 1.776 1.809 1.689  
2.5 1.246 1.223 1.235 1.115  
1.25 0.695 0.685 0.690 0.570  
0.625 0.464 0.444 0.454 0.334  
0.312 0.252 0.249 0.251 0.131  
0.156 0.187 0.185 0.186 0.066  
0 0.122 0.118 0.120    
References
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Function Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.
Subcellular Location Secreted
Protein Families Cystatin family
Tissue Specificity Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid.
Database Links

HGNC: 2473

OMIM: 123855

KEGG: hsa:1469

STRING: 9606.ENSP00000305731

UniGene: Hs.123114

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