Product Details

Target Name

myosin regulatory light chain interacting protein

Alternative Names

BM-023 ELISA Kit; BZF1 ELISA Kit; E3 ubiquitin protein ligase MYLIP ELISA Kit; E3 ubiquitin-protein ligase MYLIP ELISA Kit; Idol ELISA Kit; Inducible degrader of the LDL-receptor ELISA Kit; Lipoprotein receptor ELISA Kit; MIR ELISA Kit; MYLIP ELISA Kit; MYLIP_HUMAN ELISA Kit; Myosin regulatory light chain interacting protein ELISA Kit; PP5242 ELISA Kit

Abbreviation

MYLIP

Uniprot No.

Q8WY64

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MYLIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	100-110
1:2	Average %	96
	Range %	92-99
1:4	Average %	89
	Range %	84-94
1:8	Average %	93
	Range %	90-96

Recovery

The recovery of human MYLIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	99-103
EDTA plasma (n=4)	90	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.778	2.864	2.821	2.689
500	2.270	2.305	2.288	2.156
250	1.520	1.532	1.526	1.394
125	0.919	0.956	0.938	0.806
62.5	0.554	0.524	0.539	0.407
31.2	0.408	0.398	0.403	0.271
15.6	0.263	0.258	0.261	0.129
0	0.129	0.134	0.132

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Related Products

Target Background

Function (From Uniprot)	E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
Gene References into Functions	the effects caused by human inducible degrader of the low-density lipoprotein expression are LDLR- dependent given the unchanged plasma lipids in LAhB mice lacking low-density lipoprotein receptor PMID: 26786161 The long noncoding RNA RP1-13D10.2 may contribute to LDL cholesterol levels in response to statins. PMID: 27071970 Specifically, loss of IDOL increases LDLR distribution in the hepatic cell, and subsequently reduces serum LDL-C levels in dyslipidemic patients PMID: 26601593 Data suggest inducible expression of IDOL is subject to robust, rapid regulation by process that is sensitive to deubiquitinase inhibition in human/mouse cell lines and primary human cells; transcriptional induction of IDOL leads to degradation of LDLR. PMID: 26719329 Identify USP2 as a novel regulator of lipoprotein clearance owing to its ability to control ubiquitylation-dependent degradation of the LDLR by IDOL. PMID: 26666640 The study identified MARCH6 as a negative regulator of SREBP2-mediated transcription and described an unexpected E3 circuit functionally linking MARCH6 and IDOL to limit uptake of low-density lipoprotein via the LDLR pathway. PMID: 26527619 IDOL N342S Variant, Atherosclerosis Progression and Cardiovascular Disorders in the Italian General Population. PMID: 25927920 Several lipid-related gene polymorphisms interact with overweight/obesity to modulate blood pressure levels. PMID: 23109900 study indicates that MYLIP p.N342S might be a pharmacogenetic marker for lipid-lowering therapy in patients with FH. PMID: 25171759 Liver-specific expression of dominant-active IDOL is associated with hypercholesterolemia and a marked elevation in atherosclerotic lesions in transgenic mice. PMID: 24935961 Results show that IDOL contributes to variation in circulating levels of LDL-C. PMID: 23324548 IDOL is recruited to plasma membrane by low-density lipoprotein receptor (LDLR), promotes LDLR internalization in the absence of clathrin or caveolae, and facilitates LDLR degradation by shuttling it into the multivesicular body protein-sorting pathway PMID: 23382078 MYLIP rs3757354 SNP is associated with serum TC, HDL-C and ApoAI levels in the Bai Ku Yao and Han populations. But the association is different between the two ethnic groups. PMID: 23107276 No association of the MYLIP rs9370867 genotypes with lipid profile, hemodynamic data, and coronary angiographic data was in a Brazilian population. PMID: 22741812 FGF21 also enhanced expression of Canopy2 (Cnpy2)/MIR-interacting Saposin-like protein (Msap) that is known to interact with Mylip/Idol. PMID: 22378787 expression levels rise with increasing age in hearts of men PMID: 22261164 both the FERM and RING domains are required for promoting lysosomal degradation of the LDLR by IDOL. PMID: 21734303 N342S MYLIP polymorphism is associated with high total cholesterol and increased LDL receptor degradation in humans PMID: 21765216 identify the IDOL-UBE2D complex as an important determinant of LDLR activity, and provide insight into molecular mechanisms underlying the regulation of cholesterol uptake PMID: 21685362 Data report that statins exert opposite effects on PCSK9 and Idol gene expression in human hepatoma-derived cell lines and primary hepatocytes isolated from hamsters and rats. PMID: 21069265 MSAP interacts with this protein that enhances neurite outgrowth and increases myosin regulatory light chain in fetal and adult brain. PMID: 12826659 c-MIR is the first example of an E3 ubiquitin ligase that is capable of inhibiting major histocompatibility (MHC) class II expression in antigen-presenting cells; c-MIR might potently regulate immune responses in vivo. PMID: 16785530 study shows the LXR-Idol(Mylip)-LDLR axis defines a complementary pathway to sterol response element-binding proteins for sterol regulation of cholesterol uptake PMID: 19520913 Novel insights into the physiology of this receptor come from studies on the ubiquitination and degradation of LDL receptor by the ubiquitin ligase Mylip/Idol that is induced in cells by the nuclear receptor, LXR. PMID: 19688294 Show More Hide All
Subcellular Location	Cytoplasm. Cell membrane; Peripheral membrane protein.
Tissue Specificity	Ubiquitously expressed.
Database Links	HGNC: 21155 OMIM: 610082 KEGG: hsa:29116 STRING: 9606.ENSP00000349298 UniGene: Hs.484738

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Code	CSB-EL015319HU
Size	96T,5×96T,10×96T
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Human E3 ubiquitin-protein ligase MYLIP(MYLIP) ELISA kit

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Target Background

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