Human EGF, latrophilin and seven transmembrane domain-containing protein 1(ELTD1) ELISA kit

Instructions
Code CSB-EL007630HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name EGF, latrophilin and seven transmembrane domain containing 1
Alternative Names ADGRL4 ELISA Kit; Adhesion G protein-coupled receptor L4 ELISA Kit; EGF ELISA Kit; EGF latrophilin and seven transmembrane domain containing 1 ELISA Kit; EGF TM7 latrophilin related protein ELISA Kit; EGF; latrophilin and seven transmembrane domain-containing protein 1 ELISA Kit; EGF; latrophilin; and 7 transmembrane domain-containing protein 1 ELISA Kit; EGF-; TM7-; and latrophilin-related protein ELISA Kit; EGF-TM7-latrophilin-related protein ELISA Kit; EGFTM7 latrophilin related protein ELISA Kit; ELTD 1 ELISA Kit; ELTD1 ELISA Kit; ELTD1_HUMAN ELISA Kit; ETL ELISA Kit; ETL protein ELISA Kit; KPG_003 ELISA Kit; latrophilin and seven transmembrane domain-containing protein 1 ELISA Kit; UNQ202/PRO228 ELISA Kit; zgc:63629 ELISA Kit
Abbreviation ELTD1
Uniprot No. Q9HBW9
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ELTD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 89-98
1:2 Average % 97
Range % 91-103
1:4 Average % 92
Range % 87-98
1:8 Average % 88
Range % 85-92
Recovery
The recovery of human ELTD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 98-104
EDTA plasma (n=4) 87 82-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.116 2.231 2.174 2.012
800 1.597 1.643 1.620 1.458
400 1.166 1.187 1.177 1.015
200 0.833 0.826 0.830 0.668
100 0.551 0.563 0.557 0.395
50 0.362 0.372 0.367 0.205
25 0.281 0.287 0.284 0.122
0 0.161 0.162 0.162  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Endothelial orphan receptor that acts as a key regulator of angiogenesis.
Gene References into Functions
  1. High ELTD1 expression is associated with glioma. PMID: 27416955
  2. miR-139-5p suppresses glioblastoma multiforme cell proliferation by targeting ELTD1 and regulating cell cycle. PMID: 26449464
  3. Results provide insight into the regulation of tumor angiogenesis and highlight ELTD1 as key player in blood vessel formation. PMID: 23871637
  4. Two of these (D1S0818i-ELTD1 and D6S0035i-MAPK14) remain associated following application of Bonferroni's correction and multivariate analysis. PMID: 23474535
  5. Results of this study strongly suggests that associative analysis was able to accurately identify ELTD1 as a putative glioma-associated biomarker PMID: 23096411
  6. Genes such as ELTD1 on chromosome 1, in addition to genes on chromosomes 4 (eg, GABRA2) and 6 (eg, CNR1), may be associated with the genetic risk for cannabis use disorders. PMID: 18519829

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 2 family, Adhesion G-protein coupled receptor (ADGR) subfamily
Tissue Specificity Detected in the majority of epithelial cells in tumor and normal tissues. Expressed also in human umbilical vein endothelial cells.
Database Links

HGNC: 20822

OMIM: 616419

KEGG: hsa:64123

STRING: 9606.ENSP00000359778

UniGene: Hs.132314

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