Human ELAV-like protein 1(ELAVL1) ELISA kit

Instructions
Code CSB-EL007592HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Alternative Names ELAV (embryonic lethal abnormal vision Drosophila) like 1 ELISA Kit; ELAV (embryonic lethal; abnormal vision; Drosophila) like 1 (Hu antigen R) ELISA Kit; ELAV like 1 ELISA Kit; ELAV like RNA binding protein 1 ELISA Kit; ELAV-like protein 1 ELISA Kit; ELAV1 ELISA Kit; ELAV1_HUMAN ELISA Kit; Elavl1 ELISA Kit; Embryonic lethal abnormal vision drosophila homolog like 1 ELISA Kit; Hu Antigen R ELISA Kit; Hu-antigen R ELISA Kit; Hua ELISA Kit; HuR ELISA Kit; MelG ELISA Kit
Abbreviation ELAVL1
Uniprot No. Q15717
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ELAVL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 95    
Range % 89-99    
1:2 Average % 96    
Range % 92-101    
1:4 Average % 94    
Range % 88-98    
1:8 Average % 92    
Range % 88-96    
Recovery
The recovery of human ELAVL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 98 94-102    
EDTA plasma (n=4) 87 83-92    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
1600 2.167 2.058 2.113 1.956    
800 1.643 1.553 1.598 1.441    
400 1.166 1.137 1.152 0.995    
200 0.787 0.759 0.773 0.616    
100 0.542 0.536 0.539 0.382    
50 0.368 0.352 0.360 0.203    
25 0.279 0.271 0.275 0.118    
0 0.158 0.156 0.157      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability
Gene References into Functions
  1. B4GALT1-AS1 recruited HuR to enhance YAP mRNA stability and thus its transcriptional activity PMID: 30182452
  2. HuR associates with TERC in vitro and in cultured cells. The association of HuR with TERC was required for the maintenance of TERC methylation and hence telomerase activity. Additionally, the regulation of telomerase activity by HuR was found to impact on the renewal of hematopoietic stem cells and was linked to dyskeratosis congenita, aplastic anemia, and autosomal dominant dyskeratosis congenita. PMID: 29880812
  3. this study identified an essential Jun/miR-22/HuR regulatory axis in CRC (the working model is summarised in Fig. 8) and highlighted the vital role of HuR and miR-22 in CRC proliferation and migration. PMID: 29351796
  4. RNA immunoprecipitation and RNA pull-down assays in glioma cell lines demonstrated that SNHG12 was associated with and was stabilized by Hu antigen R. Glioma patients with high levels of SNHG12 exhibited a reduced 5-year overall survival rate. PMID: 30015836
  5. Knockdown of HuR significantly increases the apoptosis of airway epithelial cells and down-regulates ZEB-1 expression. PMID: 29866111
  6. High HUR expression is associated with Invasive Breast Carcinoma. PMID: 28808873
  7. HuR acts as a tumor promoter by enhancing YAP expression in osteosarcoma cells. PMID: 29597092
  8. Study supports HuR's role as an upstream regulator of p62 expression in ARPE-19 cells, helps to understand better the early events in response to a proautophagy stimulus. PMID: 29576851
  9. Our data...strongly suggest that HuR expression...could be used as a prognostic marker in melanoma. PMID: 29393978
  10. HuR protein is expressed in the majority of the cases in all the tumors of the head and neck region examined. More importantly, higher levels of HuR expression have been noted, showing higher cytoplasmic levels in malignant or premalignant cases. Moreover, HuR modulates several genes implicated in biological processes important for malignant transformation, growth, and invasiveness. [review] PMID: 29619127
  11. Radiation-activated ataxia-telangiectasia mutated kinase/p38 signaling positively contributed to the nucleus to cytosol translocation of HuR. PMID: 29856906
  12. that HuR is involved in the process of acquiring malignant histopathological features and ability to invade the muscularis propria by urothelial bladder cancer cells PMID: 29566946
  13. HuR facilitated lung cancer stemness dependent on CDK3 expression. miR-873 or miR-125a-3p level was negatively correlated with HuR and CDK3 expression levels in lung cancer tissues. HuR facilitates lung cancer stemness via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis. PMID: 29344850
  14. HuR plays an important role in the progression of esophageal carcinoma by targeting IL-18. PMID: 28231690
  15. the promotive effects of HuR overexpression on the inflammatory response were attenuated when HUVECs were cotreated with HMGB1 short hairpin RNA. Therefore, the present results indicated that the ectopic expression of HuR may induce inflammatory responses and thus sepsis by activating the HMGB1 signaling pathway. PMID: 29115544
  16. Computational analysis of publicly available microarray datasets of non-small cell lung cancer (NSCC) treated with EGFR targeted therapy identified ESR1 and ELAV1 as the most highly ranked hub genes involved in the NSCLC acquired TKI-resistance. PMID: 29758295
  17. Studies indicate that by regulating the mRNAs of target genes, Hu antigen R protein (HuR) is causally linked to the onset and progression of kidney diseases [Review]. PMID: 28910975
  18. The widespread distribution of intronic adenylate-uridylate (AU)-rich elements (AREs) and their particular association with ELAVL1/HuR and ELAVL1/HuR binding sites indicates that more than half of human genes can be regulated post-transcriptionally by AREs. PMID: 29413897
  19. Posttranscriptional regulation of HuR by miR-133b enhances DTX cytotoxicity through inhibition of ABCG2. PMID: 29327946
  20. HuR is required for pancreatic ductal adenocarcinoma tumor growth. PMID: 28242812
  21. Transgenic expression of HuR in astrocytes accentuated neuronal injury and other secondary features of spinal cord injury including increased vascular permeability and astrocyte activation. PMID: 28487080
  22. HuR may be involved in the pathogenesis of glaucoma. PMID: 29107807
  23. Findings reveal a new mechanism whereby HUR protects NONO from mir320-mediated degradation upon UVC exposure and identify a new component within the complex network of players underlying the DNA damage response adding mir320a to the list of p53-regulated targets upon genotoxic stress. PMID: 27816966
  24. ectopic expression of HuR promotes breast cancer cell proliferation and survival by directly binding to and stabilizing CDK3 mRNA. PMID: 28501005
  25. New ELAVL1-TYK2 fusion gene is oncogenic in acute myeloid leukemia. PMID: 27189703
  26. High expression of HuR is correlated with thyroid cancer. PMID: 27542231
  27. Folate deficiency impaired spermatogenesis and reduced sperm concentration may partly due to inhibiting the expression of the three key molecules (Esr1, Cav1 and Elavl1) essential for sperm production. PMID: 28445960
  28. these studies demonstrate that HuR knockdown in triple-negative breast cancer cells elicits oxidative stress and DNA damage resulting in radiosensitization PMID: 27588488
  29. LncRNA-AK058003 can reduce the expression of mRNA stabilizing protein HuR and act as a precursor of miR-15a to suppress gamma-synuclein-mediated cell proliferation and the metastasis of hepatocellular carcinoma. PMID: 28035067
  30. These results indicate that HuR plays an important role in adenovirus replication, at least in part, by up-regulating IVa2 mRNA expression and that AU-rich element (ARE)-mRNA stabilization is required for both transformation and virus replication. PMID: 29225167
  31. Data indicate that both ELAVL1 and TIAR positively regulate endogenous SNCA in vivo. PMID: 29149290
  32. identified HuR as a novel posttranscriptional regulator of ABCA1 expression and cellular cholesterol homeostasis, thereby opening new avenues for increasing cholesterol efflux from atherosclerotic foam macrophages and raising circulat-ing HDL cholesterol levels. PMID: 24729624
  33. HuR expression and cytoplasmic localization were increased in fibrotic livers. S1P induced migration of human bone marrow Mesenchymal Stem Cells via S1PR3 and HuR. PMID: 27543493
  34. RNA-binding protein HuR (HuR) expression negatively correlated with chemokine (CC motif) ligand 5 (CCL5) expression and macrophage appearance in a cohort of breast tumors. PMID: 28731284
  35. Data indicate no correlation between RNA-binding protein HuR intracellular localization in tumor sections and progression free survival. PMID: 26943573
  36. our research demonstrated that NEAT1 acts as a key regulator in human ovarian carcinogenesis and revealed two regulatory factors (HuR and miR-124-3p) of NEAT1 expression. PMID: 27075229
  37. The crucial role of HuR as a stabilizing factor for the MR transcript. PMID: 28744670
  38. study found cytoplasmic HuR is significantly overexpressed in higher-grade (atypical and anaplastic) than in lower-grade meningiomas and the HuR level is correlated with survival in patients with meningioma; siHuR-induced HuR knockdown was shown to reduce the growth of both Ben-Men-1 and IOMM-Lee cells PMID: 28493484
  39. combined treatments of siHuR and epirubicin significantly reduced the expression of Bcl-2, but increased the expression of Bax, as well as activity and expression levels of caspase-3 and -9. In contrast, overHuR abrogated these effects PMID: 28968471
  40. High HuR expression is associated with pancreatic ductal adenocarcinomas. PMID: 28687616
  41. GPRC5A is a potential oncogene in pancreatic ductal adenocarcinoma cells that is upregulated by gemcitabine with help from HuR. PMID: 27415424
  42. Hur role in the epithelial-to-mesenchymal transition in pancreatic cancer PMID: 28965949
  43. exposing glioma cells to the HuR inhibitor tanshinone group compound 15,16-dihydrotanshinone-I or to the newly identified compound 5 disrupts HuR multimerization modules and reduces tumor cell survival and proliferation. PMID: 28790173
  44. We propose that the extensive binding of CircPABPN1 to HuR prevents HuR binding to PABPN1 mRNA and lowers PABPN1 translation, providing the first example of competition between a circRNA and its cognate mRNA for an RBP that affects translation. PMID: 28080204
  45. HuR contributes to efficient oxidative phosphorylation by regulating of CoQ10 biosynthesis. PMID: 26690054
  46. Data (including data from studies using knockout mice or cells from knockout mice) suggest HUR post-transcriptionally regulates CCR6 expression by binding to and stabilizing CCR6 mRNA and by promoting CCR6 translation in helper T-cells; knock-out of HUR reduces CCR6 expression on helper T-cells, impairs their migration to CNS, and prevents experimental autoimmune encephalomyelitis. (CCR6 = C-C chemokine receptor type 6) PMID: 28684423
  47. Taken together, our results indicate that HuR activation is an early event in familial adenomatosis polyposis (FAP)--adenoma but is not present in inflammatory bowel disease (IBD)-dysplasia. Furthermore, our results offer a preclinical proof of concept for HuR inhibition as an effective means of FAP chemoprevention, with caution advised in the setting of IBD PMID: 28428272
  48. Negative regulation of RNA-binding protein HuR by tumor-suppressor ECRG2 PMID: 26434587
  49. Data suggest that ERBB2 is post-transcriptionally up-regulated in tamoxifen-resistant estrogen receptor-positive breast cancer cells; ERBB2 expression is decreased post-transcriptionally by miRNA26a/b binding to its 3prime- untranslated region; ERBB2 expression is increased post-transcriptionally by HuR binding to its 3prime-untranslated region. (ERBB2 = epidermal growth factor receptor 2; HuR = Hu antigen R) PMID: 28637868
  50. by modulating extracellular export of miR-122, HuR could control stress response in starved human hepatic cells. PMID: 27402548

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Subcellular Location Cytoplasm, Nucleus
Protein Families RRM elav family
Tissue Specificity Ubiquitous. Detected in brain, liver, thymus and muscle.
Database Links

HGNC: 3312

OMIM: 603466

KEGG: hsa:1994

STRING: 9606.ENSP00000385269

UniGene: Hs.184492

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