Code | CSB-EL007680HU |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human ENPP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 89 | ||||
Range % | 83-103 | |||||
1:2 | Average % | 96 | ||||
Range % | 89-103 | |||||
1:4 | Average % | 90 | ||||
Range % | 86-100 | |||||
1:8 | Average % | 89 | ||||
Range % | 82-101 |
The recovery of human ENPP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 82-104 | ||||
EDTA plasma (n=4) | 97 | 85-101 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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The product CSB-EL007680HU is a sandwich ELISA kit developed to measure concentrations of human ENPP2 in serum, plasma, or tissue homogenates. This assay uses the competitive technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops negatively to the amount of ENPP2 in samples. The color intensity is measured at 450 nm via a microplate reader.
ENPP2, also known as autotaxin (ATX), is an enzyme present in blood circulation that is responsible for the conversion of lysophosphatidylcholine (LPC) to lysophosphatidic acid (LPA). LPA is a phospholipid mediator that elicits growth-factor-like responses in almost all cell types, including cell growth, survival, differentiation, and motility. ENPP2/LPA promotes several physiological and pathological processes, including in pulmonary fibrosis, thus serving as attractive drug targets. Increased ENPP2 expression has been detected in various types of cancer, including mammary, thyroid, hepatocellular, and renal cell carcinomas, non-small-cell lung cancer, as well as glioblastoma and neuroblastoma.
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