Human Eosinophil peroxidase(EPX) ELISA kit

Instructions
Code CSB-EL007756HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name eosinophil peroxidase
Alternative Names Eosinophil peroxidase [Precursor] ELISA Kit; Eosinophil peroxidase ELISA Kit; Eosinophil peroxidase heavy chain ELISA Kit; EPER ELISA Kit; EPO ELISA Kit; EPP ELISA Kit; EPX ELISA Kit; EPX PEN ELISA Kit; PERE ELISA Kit; PERE_HUMAN ELISA Kit
Abbreviation EPX
Uniprot No. P11678
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human EPX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %80-95
1:2Average %95
Range %90-100
1:4Average %92
Range %87-99
1:8Average %97
Range %92-102
Recovery
The recovery of human EPX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9086-96
EDTA plasma (n=4)9590-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2001.952 1.872 1.912 1.818
1001.411 1.385 1.398 1.304
500.842 0.804 0.823 0.729
250.444 0.419 0.432 0.338
12.50.272 0.254 0.263 0.169
6.250.181 0.169 0.175 0.081
3.120.131 0.121 0.126 0.032
00.097 0.091 0.094  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

Function
(From Uniprot)
Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.
Gene References into Functions
  1. peroxidase enzymes, like MPO and EPO, may play a fundamental role in inhibiting RANKL-induced osteoclast differentiation at inflammatory sites of bone fracture and injury. PMID: 27836774
  2. Both MPO and EPO are causatively involved in breast cancer progression and identified as potential therapeutic targets whereby specific novel inhibitors may reduce tumor growth and limit the occurrence of metastasis. PMID: 28260049
  3. The main significance of this work is the discovery of EPO as a novel ligand for the HER2 receptor. Following HER2 activation, EPO induces activation of FAK and subsequent activation of beta1-integrin, via inside-out signaling. This complex results in downstream activation of ERK1/2 and a sustained up regulation of both MUC4 and the HER2 receptor PMID: 27519953
  4. EPO-mediated protein carbamylation is promoted during allergen-induced asthma exacerbation, and can both modulate immune responses and trigger a cascade of many of the inflammatory signals present in asthma. PMID: 27587397
  5. there is a strong association in a given patient between both nasal and pharyngeal EPX levels and the eosinophil percentage of induced sputum. PMID: 26645423
  6. Myeloperoxidase and eosinophil peroxidase are readily internalized by HUVEC cells where they promote cellular proliferation, migration, invasion, and stimulate angiogenesis both in vitro and in vivo. PMID: 26386352
  7. A preferential role of EPO signaling via a specific surface receptor that leads to neural plasticity. PMID: 24937179
  8. mRNA levels of eosinophil granule proteins, rather than sputum eosinophil%, may reflect airway hyperresponsiveness and airflow limitation. PMID: 24814827
  9. Eosinophil peroxidase in sputum represents a unique biomarker of airway eosinophilia. PMID: 23931643
  10. report the prevalence of a common SNP in the eosinophil protein x/eosinophil-derived neurotoxin (EPX/EDN, RNase2) and the association with the cellular contents of EPX/EDN and ECP PMID: 24738159
  11. Polymorphisms of EPX and ECP are associated to inflammatory bowel disease in an age and gender dependent manne. PMID: 23197886
  12. Data indicate that eosinophil peroxidase (EPX)-based ELISA is the only eosinophil-specific assay. PMID: 22750539
  13. A mechanism of induction of ASIC-3 expression relevant to AR was suggested by the finding that eosinophil peroxidase (EPO), acting via ERK1/2, induced the expression of ASIC-3 in epithelial cells. PMID: 22702502
  14. HER2 was identified as a novel mediator of eosinophil peroxidase signaling. Eosinophil peroxidase, at noncytotoxic levels, can drive cell-cycle progression and proliferation. PMID: 21454806
  15. EPO-dependent oxidative damage may play a role in tissue injury in bisulfite-exacerbated eosinophilic inflammatory disorders PMID: 20501663
  16. induces surface alteration, killing, and lysis of Mycobacterium tuberculosis PMID: 12540536
  17. results suggest that Pro358Leu in EPO is strongly involved in the development of cedar pollinosis PMID: 14657871
  18. Polymorphisms of the eosinophil peroxidase gene may be associated with Japanese cedar pollinosis. PMID: 15316147
  19. Analysis of the role of thiocyanate (SCN-), in modulating the catalytic activity of myeloperoxidase (MPO) and other members of the lactoperoxidase (LPO) and eosinophil peroxidase (EPO) PMID: 15894800
  20. The mechanisms by which eosinophil peroxidase oxidizes nitrite are reported. PMID: 16336215
  21. post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase PMID: 18694936
  22. No variation in genes eosinophil peroxidase for Atopic dermatitis pathogenesis in this German cohort. PMID: 19014520
  23. Genetic variability in the EPO gene may contribute to the susceptibility to allergic rhinitis (or related phenotypes) in the Czech population. PMID: 19439985

Show More

Hide All

Involvement in disease Eosinophil peroxidase deficiency (EPXD)
Subcellular Location Cytoplasmic granule
Protein Families Peroxidase family, XPO subfamily
Database Links

HGNC: 3423

OMIM: 131399

KEGG: hsa:8288

STRING: 9606.ENSP00000225371

UniGene: Hs.279259

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1