Human Follicular dendritic cell secreted peptide(C4orf7) ELISA kit

Instructions
Code CSB-EL003992HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chromosome 4 open reading frame 7
Alternative Names C4orf7 ELISA Kit; FDC secreted protein ELISA Kit; FDC-SP ELISA Kit; FDCSP ELISA Kit; FDSCP_HUMAN ELISA Kit; Follicular dendritic cell secreted peptide ELISA Kit; Follicular dendritic cell secreted protein ELISA Kit
Abbreviation C4orf7
Uniprot No. Q8NFU4
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.81 ng/mL-500 ng/mL
Sensitivity 1.95 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C4orf7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 101-109
1:2 Average % 98
Range % 94-103
1:4 Average % 87
Range % 84-91
1:8 Average % 109
Range % 105-114
Recovery
The recovery of human C4orf7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 84-93
EDTA plasma (n=4) 106 103-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
500 2.221 2.094 2.158 2.046
250 1.345 1.405 1.375 1.263
125 0.742 0.789 0.766 0.654
62.5 0.413 0.402 0.408 0.296
31.25 0.305 0.331 0.318 0.206
15.62 0.217 0.208 0.213 0.101
7.81 0.182 0.178 0.180 0.068
0 0.111 0.113 0.112  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Can bind to the surface of B-lymphoma cells, but not T-lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.
Gene References into Functions
  1. These studies show that TNF-alpha stimulates human FDC-SP gene transcription by targeting YY1, GATA, C/EBP2 and C/EBP3 in the FDC-SP gene promoter. PMID: 29356241
  2. LPS-induced upregulation of FDC-SP expression in human periodontal ligament cells may enhance osteoclastogenesis in periodontal disease. PMID: 26577469
  3. FDC-SP may be involved in IgA production in the tonsils of Immunoglobulin A nephropathy patients. PMID: 25953661
  4. FDC-SP overexpression inhibits osteogenic differentiation of hPDLCs. study contributes to a better understanding of biological functions governing FDC-SP-induced hPDLC differentiation. PMID: 24138099
  5. Our findings demonstrate that transfection with FDC-SP has negligible adverse effect on proliferation of hPDLCs and imply the biological function of FDC-SP as a fibroblastic phenotype stabilizer PMID: 24357406
  6. The role of C4orf7 in ovarian cancer cell morphology, motility and invasion was demonstrated. PMID: 20811673
  7. FDC-SP has a very restricted tissue distribution, expressed by activated FDCs from tonsils and TNF-alpha-activated FDC-like cell lines, but not by B cell lines, primary germinal center B cells, or anti-CD40 plus IL-4-activated B cells. PMID: 12193705
  8. Since only normal tissue was examined, these findings suggest that FDC-SP plays an important but previously unsuspected role within oral connective tissue. PMID: 16259954
  9. These results provide the first evidence for immunomodulatory activities of FDC-SP and implicate this molecule as a regulator of B cell responses. PMID: 17548624

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Subcellular Location Secreted
Tissue Specificity Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon.
Database Links

HGNC: 19215

OMIM: 607241

KEGG: hsa:260436

STRING: 9606.ENSP00000318437

UniGene: Hs.733448

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