Product Details

Target Name

chromosome 4 open reading frame 7

Alternative Names

C4orf7 ELISA Kit; FDC secreted protein ELISA Kit; FDC-SP ELISA Kit; FDCSP ELISA Kit; FDSCP_HUMAN ELISA Kit; Follicular dendritic cell secreted peptide ELISA Kit; Follicular dendritic cell secreted protein ELISA Kit

Abbreviation

C4orf7

Uniprot No.

Q8NFU4

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.81 ng/mL-500 ng/mL

Sensitivity

1.95 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C4orf7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	101-109
1:2	Average %	98
	Range %	94-103
1:4	Average %	87
	Range %	84-91
1:8	Average %	109
	Range %	105-114

Recovery

The recovery of human C4orf7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-93
EDTA plasma (n=4)	106	103-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.221	2.094	2.158	2.046
250	1.345	1.405	1.375	1.263
125	0.742	0.789	0.766	0.654
62.5	0.413	0.402	0.408	0.296
31.25	0.305	0.331	0.318	0.206
15.62	0.217	0.208	0.213	0.101
7.81	0.182	0.178	0.180	0.068
0	0.111	0.113	0.112

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human C4orf7 ELISA Kit was designed for the quantitative measurement of Human C4orf7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.81 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL.

Target Background

Function
(From Uniprot)

Can bind to the surface of B-lymphoma cells, but not T-lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

Gene References into Functions

These studies show that TNF-alpha stimulates human FDC-SP gene transcription by targeting YY1, GATA, C/EBP2 and C/EBP3 in the FDC-SP gene promoter. PMID: 29356241
LPS-induced upregulation of FDC-SP expression in human periodontal ligament cells may enhance osteoclastogenesis in periodontal disease. PMID: 26577469
FDC-SP may be involved in IgA production in the tonsils of Immunoglobulin A nephropathy patients. PMID: 25953661
FDC-SP overexpression inhibits osteogenic differentiation of hPDLCs. study contributes to a better understanding of biological functions governing FDC-SP-induced hPDLC differentiation. PMID: 24138099
Our findings demonstrate that transfection with FDC-SP has negligible adverse effect on proliferation of hPDLCs and imply the biological function of FDC-SP as a fibroblastic phenotype stabilizer PMID: 24357406
The role of C4orf7 in ovarian cancer cell morphology, motility and invasion was demonstrated. PMID: 20811673
FDC-SP has a very restricted tissue distribution, expressed by activated FDCs from tonsils and TNF-alpha-activated FDC-like cell lines, but not by B cell lines, primary germinal center B cells, or anti-CD40 plus IL-4-activated B cells. PMID: 12193705
Since only normal tissue was examined, these findings suggest that FDC-SP plays an important but previously unsuspected role within oral connective tissue. PMID: 16259954
These results provide the first evidence for immunomodulatory activities of FDC-SP and implicate this molecule as a regulator of B cell responses. PMID: 17548624

Hide All

Subcellular Location

Secreted.

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon.

Database Links

HGNC: 19215

OMIM: 607241

KEGG: hsa:260436

STRING: 9606.ENSP00000318437

UniGene: Hs.733448

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-EL003992HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human Follicular dendritic cell secreted peptide(C4orf7) ELISA kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background