Human Gamma-glutamyl hydrolase(GGH) ELISA kit

Instructions
Code CSB-EL009389HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
Alternative Names Conjugase ELISA Kit; folylpolygammaglutamyl hydrolase ELISA Kit; gamma glutamyl hydrolase ELISA Kit; Gamma-Glu-X carboxypeptidase ELISA Kit; Gamma-glutamyl hydrolase (conjugase; folylpolygammaglutamyl hydrolase) ELISA Kit; Gamma-glutamyl hydrolase ELISA Kit; GGH ELISA Kit; GGH_HUMAN ELISA Kit; gH ELISA Kit
Abbreviation GGH
Uniprot No. Q92820
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.45 ng/mL-30 ng/mL
Sensitivity 0.11 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GGH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %84-94
1:2Average %96
Range %91-100
1:4Average %92
Range %86-95
1:8Average %97
Range %89-100
Recovery
The recovery of human GGH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9488-97
EDTA plasma (n=4)10396-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
302.728 2.667 2.698 2.582
151.552 1.478 1.515 1.399
7.50.761 0.754 0.758 0.642
3.750.505 0.499 0.502 0.386
1.80.315 0.323 0.319 0.203
0.90.238 0.242 0.240 0.124
0.450.197 0.192 0.195 0.079
00.118 0.114 0.116
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.
Gene References into Functions
  1. Either methylation of CpG1 or hypermethylation of CpG2 in GGH promoter region can significantly reduce GGH mRNA expression in pediatric patients with acute leukemia. PMID: 28278270
  2. the results of our study identify GGH as an ERG subtype specific molecular marker with modest prognostic relevance, which may have clinical relevance if analyzed in combination with other molecular markers. PMID: 28146062
  3. the highest expression of GGH and EGFR was noted in the left-sided colon; the highest expression of DHFR, FPGS, TOP1 and ERCC1 was noted in the rectosigmoid, whereas TYMP expression was approximately equivalent in the right-sided colon and rectum PMID: 26676887
  4. This study shows that polymorphisms on genes related to the metabolic pathway of pemetrexed, especially, ATIC and GGH genes, would have a therapeutic implication in pemetrexed-treated patients with lung adenocarcinoma PMID: 25823786
  5. Polymorphism of GGH rs3758149 C>T is associated with response to therapy in acute lymphoblastic leukemia. PMID: 24908438
  6. An interaction term, between FPGS rs7033913 heterozygotes and GGH rs11988534 homozygotes for the minor allele, had a p-value <0.0001 and may contribute to metotrexate toxicity in rheumatoid arthritis. PMID: 24447348
  7. GG genotype of GGH -354 T > G polymorphism may have high predictive value for myelosuppression in methotrexate treated rheumatoid arthritis patients. PMID: 22763757
  8. Suggest that GGH may serve as a potential biomarker of unfavorable clinical outcomes over short-term follow-up in breast cancer. PMID: 23374458
  9. Genotyping of DHFR 829C>T and GGH -401C>T was performed using a polymerase chain reaction. PMID: 22994778
  10. The results of our study suggested the potential interest of GGH -401C>T as a predictive factor of the outcome of cervical carcinoma treated with cisplatin-based chemoradiotherapy. PMID: 23107767
  11. There was no significant difference in gamma-glutamyl hydrolase genotype or T allele frequency between the two groups (P> 0.05). PMID: 22678806
  12. Genetic polymorphism of gamma-glutamyl hydrolase in Chinese acute leukemia children and identification of a novel double nonsynonymous mutation. PMID: 22568793
  13. a SNP in the GGH gene remained associated with reduced CVD risk, with a stronger association in early onset CVD cases. PMID: 22649255
  14. This is one of the first studies to assess FPGS and GGH genetic variants in relation to plasma homocysteine. PMID: 22018726
  15. Genotypes in GGH gene of acute lymphoblastic leukemia patients were evaluated PMID: 21538980
  16. The -401C/T polymorphism in the gamma-glutamyl hydrolase may be a factor involved in the generation of relapse to disease in patients with acute lymphoblastic leukemia. PMID: 20197200
  17. Data revealed that high FPGS gene expression, low GGH gene expression and low ABCC1 gene expression in CRC tissues were predictive factors for a high reduced folate level after LV administration. PMID: 19636555
  18. data implicate GGH as a novel biomarker for bladder cancer; suggest presence of GGH & diazepam-binding inhibitor in urine serves as a rationale for developing them as urinary markers of clinical outcomes for patients treated with neoadjuvant chemotherapy PMID: 19815704
  19. Three-dimensional structure PMID: 11953431
  20. cDNA microarray analysis led to the identification of 2 novel biomarkers that should facilitate molecular diagnosis and further study of pulmonary neuroendocrine tumors. PMID: 15492986
  21. lack of dissociation of the dimer, large monomer-monomer interface, & presence of catalytically essential Tyr-36 in the homodimer interface sequences suggest that homodimer formation is required for hGH monomer to fold into an active conformation. PMID: 16945597
  22. The genotype distribution and gene frequency of the GGH gene polymorphism was studied in a Japanese population. PMID: 17409534
  23. CpG island methylator phenotype (CIMP+) in ColoRectal Cancer (CRC) is associated with low expression of GGH, suggesting involvement of the folate pathway in the development of this phenotype. PMID: 18414409

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Subcellular Location Secreted, extracellular space, Lysosome, Melanosome
Protein Families Peptidase C26 family
Database Links

HGNC: 4248

OMIM: 601509

KEGG: hsa:8836

STRING: 9606.ENSP00000260118

UniGene: Hs.78619

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