Human Gelsolin ELISA Kit

Code CSB-E08160h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Human Gelsolin (GSN) ELISA Kit quantifies GSN in human serum, plasma, cell culture supernates, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for metabolic research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.

This assay employs the quantitative sandwich enzyme immunoassay technique, in which GSN in the samples or standards are sandwiched between pre-coated GSN antibody and Biotin-conjugated GSN antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of GSN bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of GSN in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

GSN is an actin-binding protein (ABP) involved in the regulation of actin filament assembly and disassembly. Plasma GSN can sever actin filaments and is beneficial for the body to recover from disease and injury that leaks cellular actin into the blood. GSN also plays important roles in host innate immunity, activating macrophages, and localizing inflammation. Evidence has been found that GSN exerts multiple functions in the modulation of physiological and pathological cellular processes. High GSN expression was detected in prostate cancer cells and was related to tumor progression, recurrence, metastasis, and poor prognosis.

Alternative Names Actin depolymerizing factor ELISA Kit; Actin-depolymerizing factor ELISA Kit; ADF ELISA Kit; AGEL ELISA Kit; Brevin ELISA Kit; DKFZp313L0718 ELISA Kit; GELS_HUMAN ELISA Kit; Gelsolin ELISA Kit; Gsn ELISA Kit
Abbreviation GSN
Uniprot No. P06396
Species Homo sapiens (Human)
Sample Types
Detection Range
Sensitivity
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Materials provided
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human GSN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled GSN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Gene References into Functions
  1. the gelsolin N184K variant is subjected to the same aberrant proteolytic events responsible for the formation of amyloidogenic fragments in the previously characterized mutants. PMID: 27633054
  2. Induction of cell stress alters the actin cytoskeleton in intestinal epithelial cells via changes in the actin-binding proteins villin-1 and gelsolin. PMID: 29274870
  3. Gelsolin upregulation promotes radioresistance in non-small cell lung cancer cells, at least partially, through activation of phosphoinositide 3-kinase/Akt signaling. PMID: 27121073
  4. Results identify GSN as target of miR-200a which inhibits the release of microvesicles in hepatocellular carcinoma cells by functionally regulating GSN. PMID: 28440466
  5. Gelsolin enhances the invasive capacity of colon cancer cells via elevating intracellular superoxide (O2.-) levels by interacting with Cu/ZnSOD, and gelsolin gene expression positively correlates with urokinase plasminogen activator (uPA), an important matrix-degrading protease invovled in cancer invasion. PMID: 27391159
  6. these data profile the importance of the physical interaction between HPV16 E7 and gelsolin in the acquisition of the metastatic phenotype by cervical cancer cells PMID: 27072581
  7. Our study highlights gelsolin as an important pro-disseminative factor contributing to the aggressive phenotype of diffuse gastric cancer PMID: 27058427
  8. study strongly supported the contribution of the genes ITGA2B, GSN and RHOA and the two pathways "regulation of actin cytoskeleton" and "leukocyte transendothelial migration" to osteoporosis risk. PMID: 27153759
  9. SSH1 binds to gelsolin via actin filaments in the cytosolic fraction. Gelsolin promoted solubilization of actin filaments and SSH1 in cell-free assays and in cultured cells. PMID: 25451266
  10. free actin likely contributes to impaired host defense by blocking scavenger receptor binding of bacteria. PMID: 28385809
  11. analysis of how YopO disrupts normal gelsolin function to alter host actin dynamics and thus cripple phagocytosis PMID: 28280241
  12. Our present study implied that the synergistic effect of pGSN and pIgA induced glomerular fibrosis via the TGF-beta1/Smads signal transduction pathway. This might be a potential mechanism for the glomerular fibrosis observed in IgAN patients. PMID: 28208683
  13. The expression levels of GSN were observed to be reduced in colon carcinoma (CC) cells, and the reduced expression level of GSN was often associated with a poorer metastasisfree survival rate in patients with CC (P=0.04). In addition, the overexpression of GSN inhibited the invasion of CC cells in vitro. PMID: 27573444
  14. in chronic hemodialysis patients, lower pGSN levels were not associated with hospitalization, all-cause and cardio-vascular mortality, even though pGSN was inversely correlated with age, CRP and IL-6, suggesting that inflammation may influence pGSN PMID: 28114138
  15. PODXL enhances motility and invasiveness through an increase in gelsolin-actin interactions in cell protrusions. PMID: 27461278
  16. These findings suggest that gelsolin may have a role in the disease process in atopic dermatitis patients. PMID: 26318415
  17. Plasma GSN may play an important role in the development of immunoglobulin A (IgA) nephropathy. PMID: 27997897
  18. beta-Catenin seems to be involved in the regulation of gelsolin expression, which in turn affects the migratory ability of colonic cancer cells PMID: 27798885
  19. hypoxia or GSN overexpression induces HIF-1alpha expression and reduces the expression of survival markers p-Akt and Bcl-2 in H9c2 cardiomyoblast cells PMID: 27193608
  20. Increasing amount of amyloid are associated with the severity of clinical features in hereditary gelsolin amyloidosis. PMID: 27879149
  21. The rs1078305 and rs10818524 SNPs of GSN were associated with increased risk for oral squamous cell carcinoma development in a Chinese Han population PMID: 26848502
  22. Lower blood levels of gelsolin associated with progressive aortic arch calcification. PMID: 26941566
  23. Gelsolin protein, human, yielded a receiver operating characteristics value of 89% for node-positive OSCC PMID: 25223295
  24. GSN overexpression suppresses apoptosis while down-regulated GSN promotes apoptosis in hepatocellular carcinoma. PMID: 26823700
  25. Fragmented gelsolins may be associated with the pathogenesis of fibrosis in RA-ILD. PMID: 26666486
  26. gelsolin interacts with particular components of the three cytoskeleton systems and is a constituent of midbodies PMID: 26598132
  27. novel roles for actin-depolymerizing factor and cofilin-1 in regulating the remodeling and permeability of epithelial junctions PMID: 26878213
  28. The overexpression of GSN may inhibit the proliferation, adhesion ability and invasion of 786-0 clear cell renal cell carcinoma cell line. PMID: 26398833
  29. Serum-expressed apolipoprotein B-100 protein, C9 Complement, and gelsolin can be used for differential diagnosis of Barrertts esophagus and adenocarcinoma of esophagus. PMID: 26404905
  30. TGF-beta1 induced epigenetic modification of GSN could alter the EMT process in breast cancer cells. PMID: 26482896
  31. GSNs might play a regulatory role in the suppression of the tissue damage induced by acute radiation exposure PMID: 25164111
  32. We designed and synthesized siRNA against various exons in the gelsolin gene (GSN)..our finds reveal siRNA, which is derived from target gene exon, can form the complex with H3 histone to be involved in the regulation of gene expression PMID: 25600697
  33. Results show that high gelsolin levels are associated with better prognosis in ER+HER2- breast cancer and a reduction in tumor cell migration. PMID: 26408687
  34. Gelsolin expression promoted tumor-associated phenotypes by facilitating proliferative and invasive capacities of hepatocellular carcinoma(HCC) cells, which might serve as a potential therapeutic target for HCC treatment. PMID: 26149653
  35. Based on the obtained results, we propose that the gelsolin is an important cellular target for cotinine, through which this compound influences on the basic processes involved in neoplastic transformation and metastasis, such as migration and apoptosis PMID: 25544037
  36. Group B Streptococcus-beta-haemolysin is solely responsible for gelsolin increase causing, through membrane permeability defects, calcium influx and calpain activation. PMID: 25130983
  37. Plasma gelsolin levels decrease in the blood of type II diabetics. Recombinant gelsolin helped in improving glycemic control in diabetic mice. PMID: 25478578
  38. Data show that gelsolin (GSN) plays an important role in the regulation of gynecological cell fate as reflected in dysregulation in chemosensitivity. PMID: 25246592
  39. Data indicate gelsolin in contributing to the invasive potential of LNCaP prostate adenocarcinoma cells. PMID: 25581609
  40. Gelsolin levels were a useful tool to predict functional outcome and mortality after aneurysmal subarachnoid hemorrhage. PMID: 23880145
  41. increased plasma levels after allergen-specific immunotherapy PMID: 24980225
  42. Patients developing cardiopulmonary bypass acute lung injury had lower plasma gelsolin reservoir and a much more amount and rapid consumption of plasma gelsolin early after operation PMID: 25126004
  43. This study improves the knowledge of the genetic features of Mexican patients with corneal stromal dystrophies by identifying mutations in the TGFBI, CHST6, and GSN genes. PMID: 24801599
  44. GSN is important for chemoresistance in head-and-neck cancer PMID: 24771612
  45. we predict that the gene silencing of GSN and/or the downstream blocking of GSN along the p38 pathway could be applied to ameliorate pathological cardiac hypertrophy in the future PMID: 24505034
  46. Ceruloplasmin and gelsolin are closely interacted with the oncogene NF-kappab. PMID: 23925487
  47. Plasma gelsolin is lower in normal pregnancy than in non-pregnant women. It is also significantly lower in pre-eclampsia than in normal pregnancy. PMID: 24239294
  48. This study found significantly lower plasma gelsolin levels in patients with systemic lupus erythematosus and rheumatoid arthritis compared with healthy controls PMID: 24122723
  49. Expression of mutant form of human gelsolin in mice under the control of a muscle-specific promoter induced myopathic changes reminiscent of human inclusion body myositis. PMID: 24047347
  50. Gelsolin can constitute a barrier that restricts HIV-1 infection of CD4+ lymphocytes in a pre-fusion step. PMID: 23575248

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Involvement in disease Amyloidosis 5 (AMYL5)
Subcellular Location Isoform 2: Cytoplasm, cytoskeleton, SUBCELLULAR LOCATION: Isoform 1: Secreted
Protein Families Villin/gelsolin family
Tissue Specificity Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Database Links

HGNC: 4620

OMIM: 105120

KEGG: hsa:2934

STRING: 9606.ENSP00000362924

UniGene: Hs.522373

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