Target Name
serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
Alternative Names
GDN ELISA Kit; GDN_HUMAN ELISA Kit; GDNPF ELISA Kit; Glia derived nexin ELISA Kit; Glia-derived nexin ELISA Kit; Glial derived neurite promoting factor ELISA Kit; P17 ELISA Kit; Peptidase inhibitor 7 ELISA Kit; Pi-7 ELISA Kit; Plasminogen activator inhibitor type 1; member 2 ELISA Kit; PN-1 ELISA Kit; PN1 ELISA Kit; PNI ELISA Kit; Protease inhibitor 7 ELISA Kit; Protease nexin 1 ELISA Kit; Protease nexin I ELISA Kit; Serpin E2 ELISA Kit; Serpin family E member 2 ELISA Kit; Serpin peptidase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 2 ELISA Kit; SERPINE 2 ELISA Kit; Serpine2 ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | | |
Inter-assay Precision (Precision between assays): CV%<10% | | | | |
Three samples of known concentration were tested in twenty assays to assess. | | | |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| Sample | Serum(n=4) | | |
1:1 | Average % | 85 | | |
Range % | 82-87 | | |
1:2 | Average % | 96 | | |
Range % | 93-99 | | |
1:4 | Average % | 98 | | |
Range % | 94-103 | | |
1:8 | Average % | 97 | | |
Range % | 93-100 | | |
Recovery
The recovery of human SERPINE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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Sample Type | Average % Recovery | Range | | |
Serum (n=5) | 88 | 84-92 | | |
EDTA plasma (n=4) | 107 | 103-110 | | |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.281 | 2.108 | 2.195 | 2.065 | | | 10 | 1.405 | 1.432 | 1.419 | 1.289 | | | 5 | 0.822 | 0.801 | 0.812 | 0.682 | | | 2.5 | 0.483 | 0.468 | 0.476 | 0.346 | | | 1.25 | 0.365 | 0.372 | 0.369 | 0.239 | | | 0.625 | 0.287 | 0.298 | 0.293 | 0.163 | | | 0.312 | 0.222 | 0.220 | 0.221 | 0.091 | | | 0 | 0.131 | 0.129 | 0.130 | | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Human SERPINE2 ELISA Kit was designed for the quantitative measurement of Human SERPINE2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.