Recombinant Human Glia-derived nexin(SERPINE2)

Code CSB-EP021082HU
Size US$1726
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names SERPINE2
Uniprot No. P07093
Research Area Cardiovascular
Alternative Names GDN; GDN_HUMAN; GDNPF; Glia derived nexin; Glia-derived nexin; Glial derived neurite promoting factor; P17; Peptidase inhibitor 7; Pi-7; Plasminogen activator inhibitor type 1; member 2; PN-1; PN1; PNI; Protease inhibitor 7; Protease nexin 1; Protease nexin I; Serpin E2; Serpin family E member 2; Serpin peptidase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 2; SERPINE 2 ; Serpine2
Species Homo sapiens (Human)
Source E.coli
Expression Region 20-398aa
Target Protein Sequence SHFNPLSLEELGSNTGIQVFNQIVKSRPHDNIVISPHGIASVLGMLQLGADGRTKKQLAMVMRYGVNGVGKILKKINKAIVSKKNKDIVTVANAVFVKNASEIEVPFVTRNKDVFQCEVRNVNFEDPASACDSINAWVKNETRDMIDNLLSPDLIDGVLTRLVLVNAVYFKGLWKSRFQPENTKKRTFVAADGKSYQVPMLAQLSVFRCGSTSAPNDLWYNFIELPYHGESISMLIALPTESSTPLSAIIPHISTKTIDSWMSIMVPKRVQVILPKFTAVAQTDLKEPLKVLGITDMFDSSKANFAKITTGSENLHVSHILQKAKIEVSEDGTKASAATTAILIARSSPPWFIVDRPFLFFIRHNPTGAVLFMGQINKP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 57.9kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.
Gene References into Functions
  1. Study identified a secreted protein, SerpinE2 or glia-derived nexin, as causative for the highly invasive potential of slow-cycling melanoma cells. PMID: 28925403
  2. miR-199a-3p overexpression inhibited the expression of the extracellular serine protease inhibitor E2 (SerpinE2). PMID: 28677735
  3. The role of PN-1 in the pathogenesis of intervertebral disk degeneration PMID: 27460424
  4. SerpinE2 is required in the extracellular milieu of breast tumors where it acts in multiple ways to regulate tumor matrix deposition, thereby controlling tumor cell dissemination. PMID: 27793045
  5. Single nucleotide polymorphisms in EPHX1, GSTP1, SERPINE2, and TGFB1 contributing to the quantitative traits of chronic obstructive pulmonary disease in Chinese Han population. PMID: 27193053
  6. high expression of SerpinE2 in osteosarcoma stimulates cell proliferation, promotes drugresistance, and results in poor survival by regulating CDK4 and cyclin D1. PMID: 27221371
  7. The results indicate that SERPINE2 and SLPI play a significant role in the development of papillary thyroid cancer and imply that the evaluation of serum concentrations of both anticoagulant molecules may be considered as additional marker for the differentiation of malignancies during the preoperative diagnosis of patients with thyroid gland tumors. PMID: 28255192
  8. We filtered four OSCC genes including SERPINB9, SERPINE2, GAK, and HSP90B1 through the gene global prioritization score (P < 0.005). PMID: 26318431
  9. together, these data demonstrate that SERPINE2 might prevent cartilage catabolism by inhibiting the expression of MMP-13, one of the most relevant collagenases, involved in cartilage breakdown in OA. PMID: 26305372
  10. The results suggest that the rs3795879 polymorphism of SERPINE2 is not a risk factor for chronic obstructive pulmonary disease. PMID: 26214473
  11. aberrant Serpine2/Pn-1 is required for proliferation of human and mouse medulloblastoma cells. PMID: 25901736
  12. Our findings indicate that upregulated SERPINE2 may contribute to the aggressive phenotype of gastric cancer and suggest that SERPINE2 can be used as a novel prognostic factor and anticancer target in patients with gastric cancer. PMID: 25359682
  13. MAGI2, SERPINE2, and NT5C3B expression levels are associated with airway wall thickening and additionally with bronchial inflammation, emphysema, and lung function, respectively, all features of chronic obstructive pulmonary disease. PMID: 25517131
  14. Haplotypes carrying major alleles of rs729631 (C), rs975278 (G), rs7583463 (C) of SERPINE2 had protective effect on lung function amongst South Indian male smokers with COPD. PMID: 24587150
  15. role in lung homeostasis and pulmonary fibrosis PMID: 25199049
  16. SERPINE2 and PLAU are involved in cumulus expansion and oocyte maturation. PMID: 24023701
  17. Studies suggest that protease nexin 1 as a marker of prostate cancer. PMID: 23385179
  18. PN1 regulates Hh signaling by decreasing protein levels of the Hh ligand Sonic (SHH) and its downstream effectors. PMID: 23041623
  19. crystal structures of PN1 in complex with heparin and catalytically inert thrombin suggest a unique 2-step mechanism of thrombin recognition involving rapid electrostatics-driven association PMID: 22618708
  20. PN-1 has direct antiangiogenic properties and is a yet-unrecognized player in the angiogenic balance PMID: 22331468
  21. Data suggested that there was no significant association between SERPINE2 polymorphism and COPD susceptibility in the Chinese Han population. PMID: 22028159
  22. SERPINE2 gene is a chronic obstructive pulmonary disease susceptibility gene, and block 1 of SERPINE2 appears to be the genetic variant region that affects the Han Chinese. PMID: 21611750
  23. The variant allele of the rs729631 SNP was found to pose over two-fold risk for overall panlobular changes and over four-fold risk for pathological panlobular changes. PMID: 22145704
  24. Results weakly support SERPINE2 as a Dutch hypothesis candidate gene through nominally significant associations with asthma and related traits. PMID: 21436250
  25. data identify the temporal and spatial SERPINE2 distribution in the human placenta and suggest its possible role in modulating tissue remodeling of extravillous trophoblasts in the placenta during pregnancy. PMID: 21806836
  26. SERPINE2 protein is highly expressed in the endometrium during the secretory phase, indicating that it may participate in tissue remodeling involved in implantation. PMID: 21426587
  27. platelet PN-1 can be considered as a new important regulator of thrombolysis in vivo PMID: 21403095
  28. In cerebellum, thrombin can activate PAR-1 expression after ICH, and PN-1 appears quickly after Intracerebral hemorrhage in order to control the deleterious effect of thrombin. PMID: 20819545
  29. data indicate that serpinE2 is up-regulated by oncogenic activation of Ras, BRAF and MEK1 and contributes to pro-neoplastic actions of ERK signaling in intestinal epithelial cells. PMID: 20942929
  30. SERPINE2 may be a risk factor for the development of emphysema and its association with emphysema may be stronger in smokers. PMID: 21067581
  31. SERPINE2 was overexpressed in the ER-negative breast cancer group. PMID: 20805453
  32. human astrocytes express Pn-1 and astrocytic expression is regulated in a dynamic manner by injury-related factors PMID: 20623540
  33. SERPINE2, was previously identified as a COPD susceptibility gene in the chromosome 2q linkage region. Using independent genotyping data in 2 smaller studies in Boston and Norway the results were replicated. PMID: 20463177
  34. SERPINE2 was identified as a possible promoter of testicular germ cell tumors lymph node metastasis. PMID: 20035713
  35. Results suggest that the SERPINE2 gene contributes to the susceptibility to COPD. PMID: 19949669
  36. Platelet PN-1 is a key player in the thrombotic process, whose negative regulatory role has been, up to now, markedly underestimated. PMID: 19855083
  37. Elevated levels of GDN/PN1 and XIP mRNAs induced by Allitridi provide valuable molecular evidence for elucidating garlic's efficacies against neurodegenerative and inflammatory diseases. PMID: 11925594
  38. PN1 may play a much larger role in the modeling and remodeling of brain tissues during development and is not simply an extravasated thrombin clearance mechanism as previously suggested PMID: 12356769
  39. the 4G/4G-PAI-1 genotype might be a protective factor against atherothrombotic cerebral infarction (ACI), whereas the factor V point mutation (1691G-A) and the factor VII Arg/Gln353 gene polymorphism have not proved to be risk factors for ACI PMID: 12859287
  40. The pericyte expression of protease nexin 1 may provide endogenous anticoagulant activity. PMID: 16015279
  41. Arg15, Phe34, Pro13, and Arg20 are important for FXIa inhibition by PN2 Kunitz protease inhibitor domain PMID: 16085935
  42. SERPINE2 is a COPD-susceptibility gene and is likely influenced by gene-by-smoking interaction. PMID: 16358219
  43. This study shows that syndecan-1-mediated internalization of protease nexin-1 stimulated the Ras-ERK signaling pathway. PMID: 16741952
  44. Expression array analysis demonstrated association and repication of SERPINE2 as a potential COPD susceptibility gene. PMID: 16921128
  45. Protease nexin-1 forms complexes with thrombomodulin and modulates its anticoagulant activity. PMID: 17379830
  46. provides support for SERPINE2 as a COPD susceptibility gene PMID: 17446335
  47. Co-injection of pancreatic stellate cells and tumor cells dramatically enhances the invasive potential of SERPINE2-expressing SUIT-2 cells in nude mice. PMID: 17703087
  48. Macrophages and platelets are the major source of protease nexin-1 in human atherosclerotic plaque. PMID: 18617644
  49. in severe COPD, SNPs in EPHX1 and SERPINE2 were associated with hypoxemia PMID: 19017876
  50. Here, we report that nexin-1 inhibits trypsin-4, and forms stable complexes only with this trypsin-isoenzyme. This result suggests that nexin-1 could modulate trypsin activity in brain where both nexin-1 and trypsin-4 are expressed. PMID: 19249338
  51. Results suggest that PN-1 might become a prognostic marker in breast cancer. PMID: 19584287
  52. Our results failed to obtain the evidence that these SNPs in SERPINE2 contributed to the chronic obstructive pulmonary disease susceptibility in the Han Chinese population PMID: 19604412

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Subcellular Location Secreted, extracellular space
Protein Families Serpin family
Database Links

HGNC: 8951

OMIM: 177010

KEGG: hsa:5270

STRING: 9606.ENSP00000415786

UniGene: Hs.38449

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