Human Glycated Albumin,GA ELISA Kit

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Human Glycated Albumin,GA ELISA Kit

Name: Human Glycated Albumin,GA ELISA Kit
Brand: CUSABIO
SKU: CSB-E09599h

Citations (4) Protocol

Code	CSB-E09599h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (4)
Customer Reviews and Q&A

Target Name

Glycated Albumin,GA

Alternative Names

N/A

Abbreviation

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

18.75 pmol/mL-1200 pmol/mL

Sensitivity

4.7 pmol/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	94
	Range %	90-98
1:400	Average %	94
	Range %	91-98
1:800	Average %	101
	Range %	98-104
1:1600	Average %	88
	Range %	82-96

Recovery

The recovery of human GA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	96	92-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/ml	OD1	OD2	Average
1200	0.080	0.081	0.081
600	0.117	0.118	0.118
300	0.182	0.191	0.187
150	0.285	0.276	0.281
75	0.509	0.499	0.504
37.5	0.933	0.923	0.928
18.75	1.461	1.551	1.506
0	2.539	2.668	2.604

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

Glycated albumin is a biomarker formed through non-enzymatic glycation of serum albumin when exposed to glucose over time. This modified protein reflects average glycemic control over a shorter period compared to hemoglobin A1c, making it valuable for monitoring diabetes management and assessing glycemic status in patients with altered red blood cell turnover. Glycated albumin forms through the Maillard reaction. Reducing sugars bind to amino groups on albumin, creating stable glycation products that correlate with blood glucose concentrations.

The Human Glycated Albumin ELISA Kit (CSB-E09599h) provides quantitative measurement of GA in human serum and plasma samples using a competitive assay principle. This kit offers a detection range of 18.75 pmol/mL to 1200 pmol/mL with a sensitivity of 4.7 pmol/mL, requiring 50-100 μL sample volume per test. The assay can be completed within 1-5 hours with detection at 450 nm wavelength. Researchers can accurately quantify glycated albumin levels in Homo sapiens samples for glycemic research applications.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research studies focused on glycated albumin quantification in human serum and plasma samples. The applications span metabolic research and biomarker studies where glycated albumin serves as an analytical target for understanding protein modification processes.

• Metabolic research: Quantification of glycated albumin levels in human serum samples as part of biochemical parameter analysis alongside other metabolic markers
• Biomarker studies: Measurement of glycated albumin concentrations in plasma samples with normalization to albumin levels for comparative analysis in research settings
• Protein modification research: Analysis of glycated albumin as one of several post-translational modifications including carbamoylation and pentosidine formation in pooled samples
• Clinical biochemistry: Integration of glycated albumin measurements into broader panels of biochemical parameters for research characterization of study participants

Comparison of Glycated Albumin and Glycated Hemoglobin in Type 2 diabetic Patients with End-stage of Renal Disease AE Abdrabo, I Elgaily,Thesis&Dissertation,/
Nanopore Sequencing of RAGE Gene Polymorphisms and Their Association with Type 2 Diabetes AH Chaurasiya,ACS Omega,2023
Glycemic control is not related to cerebral small vessel disease in neurologically asymptomatic individuals with type 1 diabetes Jussi Inker,Acta diabetologica,2021
A next generation setup for pre-fractionation of non-denatured proteins reveals diverse albumin proteoforms each carrying several post-translational modifications Rhode H, et al,Scientific Reports,2019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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■ Q&A

I want to ask about the product CSB-E09599h. If you have any other additional evidence concerning the validation of your method. Especially I would be interested in knowing the correlation with other GA methods, particularly with the most used enzymatic assay.

Our kit uses ELISA method, which is only for scientific research. For the use of this product CSB-E09599h, we have customers who have published literature, please check the citation part from this product page:https://www.cusabio.com/ELISA-Kit/Human-Glycated-AlbuminGA-ELISA-Kit-79507.html
So sorry we have not made comparisons with other methodologies.
The principles of different methods are also different, which is not comparable.

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