Human Glycoprotein hormones alpha chain(CGA) ELISA kit

Code CSB-EL005293HU
Size 96T,5×96T,10×96T
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Product Details

Alternative Names
Anterior pituitary glycoprotein hormones common subunit alpha ELISA Kit; CG alpha ELISA Kit; CG-alpha ELISA Kit; cga ELISA Kit; Choriogonadotropin alpha chain ELISA Kit; Chorionic Gonadotrophin Alpha ELISA Kit; Chorionic Gonadotrophin Alpha Subunit ELISA Kit; Chorionic gonadotrophin subunit alpha ELISA Kit; chorionic gonadotropin, alpha polypeptide ELISA Kit; Follicle-stimulating hormone alpha ELISA Kit; Follicle-stimulating hormone alpha chain ELISA Kit; follicle-stimulating hormone alpha subunit ELISA Kit; Follitropin alpha ELISA Kit; Follitropin alpha chain ELISA Kit; FSH Alpha ELISA Kit; FSH-alpha ELISA Kit; FSHA ELISA Kit; GLHA_HUMAN ELISA Kit; Glycoprotein hormones alpha chain ELISA Kit; Glycoprotein Hormones Alpha Polypeptide ELISA Kit; GPH Alpha ELISA Kit; GPHa ELISA Kit; GPHA1 ELISA Kit; HCG ELISA Kit; hyrotropin alpha ELISA Kit; LH-alpha ELISA Kit; LHA ELISA Kit; LSH alpha ELISA Kit; LSH-alpha ELISA Kit; Luteinizing hormone alpha ELISA Kit; Luteinizing hormone alpha chain ELISA Kit; Lutropin alpha ELISA Kit; Lutropin alpha chain ELISA Kit; Thyroid Stimulating Hormone Alpha ELISA Kit; Thyroid Stimulating Hormone Alpha Chain ELISA Kit; Thyroid-stimulating hormone alpha chain ELISA Kit; Thyrotropin alpha chain ELISA Kit; TSH Alpha ELISA Kit; TSH-alpha ELISA Kit; TSHA ELISA Kit
Abbreviation
CGA
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates
Detection Range
4 mIU/mL-120 mIU/mL
Sensitivity
1 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CGA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 92    
Range % 89-98    
1:2 Average % 95    
Range % 88-99    
1:4 Average % 94    
Range % 90-101    
1:8 Average % 95    
Range % 86-100    
Recovery
The recovery of human CGA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 91-100    
EDTA plasma (n=4) 96 91-99    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
mIU/ml OD1 OD2 Average Corrected    
120 2.525 2.501 2.513 2.466    
50 1.299 1.323 1.311 1.264    
20 0.620 0.613 0.617 0.570    
8 0.305 0.317 0.311 0.264    
4 0.157 0.152 0.155 0.108    
0 0.046 0.048 0.047      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

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Target Background

Function
(From Uniprot)
Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH, follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.
Gene References into Functions
  1. This study which performed for the first time in Iran suggests that polymorphism in CGA gene can affect male infertility. PMID: 29627965
  2. Our findings support a model in which CREB3L1 acts as a downstream effector of TSH to regulate the expression of cargo proteins, and simultaneously increases the synthesis of transport factors and the expansion of the Golgi to synchronize the rise in cargo load with the amplified capacity of the secretory pathway PMID: 29093023
  3. men with higher and women with lower serum TSH levels are at increased risk of frailty. PMID: 27869535
  4. Inhibin-B secretion and FSH isoform distribution may play an integral part of follicular selection in the natural menstrual cycle. (Review) PMID: 27756855
  5. proteomic study reveals unique protein changes in both cellular (98% unique identifications) and secreted (96% unique identifications) proteins in the transition to the progesterone-dominated secretory (receptive) phase and subsequently to pregnancy, mediated by embryo-derived human chorionic gonadotropin (hCG) PMID: 27262222
  6. the in vivo bioactivities of FSH glycoforms in Fshb null mice using a pharmacological rescue approach, was investigated. PMID: 27561202
  7. In girls with central precocious puberty, increased body mass index (BMI) was associated with slightly lower peak stimulated luteinizing hormone (LH) levels at early pubertal stages (Tanner stages 2 and 3). PMID: 27215137
  8. the distribution of 1(st) trimester TSH and evaluate its association with perinatal outcomes and future development of maternal thyrotoxicosis, is reported. PMID: 27351808
  9. The present results have for the first time shown protective effects exerted by hCG on vascular endothelial function. PMID: 26279419
  10. Human chorionic gonadotropin provides a mechanism to bridge embryo to endometrium through beta1,4-GalT. PMID: 26191157
  11. thyroid-stimulating hormone elevations can obviously be the initial finding in pseudohypoparathyroidism type Ib patients. PMID: 25403028
  12. TBP attenuates Msx1-mediated glycoprotein hormone alpha transcriptional repression. PMID: 26505791
  13. The results indicate that gal-3 might be regulated by Human chorionic gonadotropin (hCG)in preparing the endometrium for embryonic implantation. PMID: 21705042
  14. Limitations in the process of transcription and translation inhibit recombinant human chorionic gonadotropin expression in CHO cells. PMID: 25529346
  15. the 5-aminoimidazole-4-carboxyamide ribonucleoside (AICAR)-induced activation of AMPK directly inhibited the expression of SREBP-2 and HMGCR and HMGCS, and suppressed the TSH-stimulated up-regulation of SREBP-2 in HepG2 cells. PMID: 25933205
  16. Serum TSH was associated with pulse wave velocity on population level when adjusted with age and sex. PMID: 25185682
  17. A single low TSH at baseline was associated with increased risk of hip fractures (adj HR 1.16, 95% CI 1.07-1.26, p < 0.001) but not major osteoporotic fractures (MOF, adj HR 1.06, 95% CI 0.99-1.12, p = 0.058) over a median follow-up of 7.5 years. PMID: 24723381
  18. This study showed a link between low maternal serum hCG levels and cryptorchidism in boys from uncomplicated pregnancy. PMID: 25064170
  19. A protective effect of hCG against breast cancer may be associated with its growth inhibitory and differentiation induction function in breast cancer cells. PMID: 24753159
  20. LH, progesterone, and TSH can stimulate aldosterone. PMID: 24297486
  21. Elevated total hCG and free beta-hCG concentrations during early pregnancy are associated with nonstatistically significant reductions in maternal risk of breast cancer. PMID: 24559445
  22. chromogranin A PMID: 23153859
  23. The purpose of these studies was to determine if CTNNB1's contribution to FSH-mediated steroidogenesis in primary rat granulosa cells was due in part to extracellular stimulation of the canonical WNT signaling pathway. PMID: 24466091
  24. Hypo-glycosylated human follicle-stimulating hormone is much more active in vitro than its fully-glycosylated counterpart. PMID: 24291635
  25. New information about TSH signaling in human differentiated adipocytes, and add to the evidence that TSH is a pro-inflammatory stimulus of adipocytes. PMID: 24661543
  26. Human chorionic gonadotropin hormone stimulates trophoblast invasion independently of the hCG receptor. PMID: 23684886
  27. data further identified a complex interaction between IL1 and hCG, which, despite a synergistic action on several significant endometrial target genes, may encompass a tight control of endogenous IL1 and extends to other IL1 family members PMID: 23717664
  28. Msx1 functions as a negative regulator early in pituitary development by repressing the gonadotrope-specific alphaGSU and GnRHR genes. PMID: 23371388
  29. BK(Ca) is a crucial part of the signaling cascade of LH/hCG in Leydig cells. PMID: 23267835
  30. In our multi-ethnic cohort of euthyroid obese children and adolescents increasing TSH was associated with impaired glucose metabolism and dyslipidemia. PMID: 23525871
  31. Data indicate that neither serum inhibin B nor follicle-stimulating hormone (FSH) is a suitable surrogate for determination of sperm concentration in a semen sample. PMID: 23423746
  32. Ser680 genotype for FSHR is a factor of relative resistance to FSH stimulation resulting in slightly higher FSH serum levels, thus leading to a prolonged duration of the menstrual cycle. PMID: 23380184
  33. The use of these age-specific reference intervals for TSH, especially in those over 70 years old, would result in the reclassification of many TSH results from "abnormal" to "normal" and avoid unnecessary treatment. PMID: 23345094
  34. hCG coordinates embryonic signal interaction with the maternal endometrium and may promote embryonic growth. PMID: 22811570
  35. N-acetyl-L-cysteine modulates multiple signaling pathways to rescue male germ cells from apoptosis induced by chorionic gonadotropin. PMID: 22354444
  36. Data suggest that in embryonic stem cells [which express proto-oncogene c-ETS-2 (ETS2) but not CGA], ETS2 does not occupy its binding site on CGA promoter but is found instead as soluble complex with POU5F1 (Oct3 transcription factor). PMID: 22446105
  37. low urinary level of hyperglycosylated hCG is associated with pregnancy failure PMID: 22386126
  38. second-trimester maternal serum levels of alpha-fetoprotein, human chorionic gonadotropin, and unconjugated estriol may have a role in bronchopulmonary dysplasia PMID: 22391642
  39. Data suggest that a progressive increase in TSH are predictive factors for thyroid failure in Hashimoto's thyroiditis (HT) patients. PMID: 21981142
  40. Data suggest that likelihood of papillary thyroid carcinoma is reduced when serum TSH is lower, as in thyroid autonomy, and increased when serum TSH is higher, as in thyroid autoimmunity. [Meta-Analysis; REVIEW] PMID: 22278420
  41. TSH showed significant positive correlation with creatinine and uric acid values and, thyroid function test had a negative correlation with uric acid in overt hypothyroidism. PMID: 22061337
  42. Serum IL-6, IL-1beta and hCG levels were significantly increased in women with pre-eclampsia compared to controls. PMID: 21623995
  43. TSH-TSHR interaction through cAMP signaling (using thyrogen/rTSHalpha) is involved in differentiation/gene expression regulation of bone marrow-derived mesenchymal stem cells; first evidence of expression of TSHR in stem cell plasma membrane. PMID: 22128326
  44. investigation of hCG in seminal plasma: Level of free hCGa is reduced in infertility with abnormal semen analysis. In oligospermia, holo-hCG is low; hCGa is present as highly glycosylated large free subunit with a unique glycosylation pattern. PMID: 21838882
  45. TSH is a novel neuroendocrine regulator of selected keratins in the human hair follicle PMID: 21763112
  46. Increasing concentrations of FSH caused a biphasic dose-response, with a maximal (1.5-fold) increase in RANK(high) cells. PMID: 21159522
  47. In euthyroid children without a history of hypo- or hyperthyroidism, increasing levels of TSH and decreasing levels of free T4 are associated with higher triglyceride levels and elevated markers of insulin resistance. PMID: 21114382
  48. Results suggest that a serum TSH concentration at the lower end of the reference range may be associated with low BMD in men. PMID: 20455884
  49. Findings demonstrate that FSH has dose-dependent anabolic effects on bone via an ovary-dependent mechanism. PMID: 21149714
  50. Women with high TSH at end term are at risk for breech presentation. Substantial evidence for a relation between breech presentation and neurodevelopmental delay exists. PMID: 20718770

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Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit alpha family
Database Links

HGNC: 1885

OMIM: 118850

KEGG: hsa:1081

STRING: 9606.ENSP00000358595

UniGene: Hs.119689

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