Human HLA-B associated transcript 3 (BAT3) ELISA kit

Code CSB-EL002567HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
HLA-B associated transcript 3
Alternative Names
2410045D21Rik ELISA Kit; AA408914 ELISA Kit; BAG 6 ELISA Kit; BAG family molecular chaperone regulator 6 ELISA Kit; BAG-6 ELISA Kit; BAG6 ELISA Kit; BAG6_HUMAN ELISA Kit; BAT 3 ELISA Kit; BAT3 ELISA Kit; BCL2-associated athanogene 6 ELISA Kit; D17H6S52E ELISA Kit; D6S52E ELISA Kit; G3 ELISA Kit; HLA B associated transcript 3 ELISA Kit; HLA-B associated transcript 3 ELISA Kit; HLA-B associated transcript-3 ELISA Kit; HLA-B-associated transcript 3 ELISA Kit; large proline rich protein BAG6 ELISA Kit; Large proline rich protein BAT3 ELISA Kit; Large proline-rich protein BAG6 ELISA Kit; large proline-rich protein BAT3 ELISA Kit; Protein G3 ELISA Kit; Protein Scythe ELISA Kit; Scythe ELISA Kit; Scythe; homolog of Xenopus ELISA Kit
Abbreviation
BAT3
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human BAT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %90-98
1:2Average %96
Range %91-101
1:4Average %90
Range %87-95
1:8Average %98
Range %96-106
Recovery
The recovery of human BAT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9285-96
EDTA plasma (n=4)8782-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.086 2.210 2.148 2.055
20001.517 1.587 1.552 1.459
10000.947 0.932 0.940 0.847
5000.581 0.599 0.590 0.497
2500.380 0.390 0.385 0.292
1250.254 0.246 0.250 0.157
62.50.173 0.177 0.175 0.082
00.093 0.092 0.093  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human BAT3 ELISA Kit was designed for the quantitative measurement of Human BAT3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

Citations

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Target Background

Function
(From Uniprot)
ATP-independent molecular chaperone preventing the aggregation of misfolded and hydrophobic patches-containing proteins. Functions as part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, which maintains these client proteins in a soluble state and participates in their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation. The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum. Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated by RNF126, an E3 ubiquitin-protein ligase associated with BAG6 and are sorted to the proteasome. SGTA which prevents the recruitment of RNF126 to BAG6 may negatively regulate the ubiquitination and the proteasomal degradation of client proteins. Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome. BAG6 is also required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, it may participate in the production of antigenic peptides and play a role in antigen presentation in immune response. BAG6 is also involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. BAG6 may ensure the proper degradation of these proteins and thereby protects the endoplasmic reticulum from protein overload upon stress. By inhibiting the polyubiquitination and subsequent proteasomal degradation of HSPA2 it may also play a role in the assembly of the synaptonemal complex during spermatogenesis. Also positively regulates apoptosis by interacting with and stabilizing the proapoptotic factor AIFM1. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway.; Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).; Released extracellularly via exosomes, it is a ligand of the natural killer/NK cells receptor NCR3 and stimulates NK cells cytotoxicity. It may thereby trigger NK cells cytotoxicity against neighboring tumor cells and immature myeloid dendritic cells (DC).; Mediates ricin-induced apoptosis.
Gene References into Functions
  1. The observations suggest a mechanism whereby the BAG6 ubiquitin-linked domain provides a platform for discriminating substrates with shorter hydrophobicity stretches as a signal for defective proteins. PMID: 26663859
  2. BAG6 co-localizes with HSPA2 in huinan testicular germ cells and epididymal spermatozoa, moving from the equator to the anterior head during capacitation and stably interacting with HSPA2. In zona pellucida binding defect infertility, BAG6 is lacking. PMID: 26153132
  3. we found BAG6 to be dispensable in antigen processing PMID: 26598275
  4. VNTR in the coding region of the FAM46A gene, FAM46A rs11040 SNP and BAG6 rs3117582 SNP are associated with susceptibility to tuberculosis PMID: 24625963
  5. BAG6 rs3117582 SNP was associated with non small cell lung cancer in the Norwegian subjects and the combined Croatian-Norwegian subjects corroborating the earlier finding that BAG6 rs3117582 SNP was associated with lung cancer in Europeans. PMID: 25884493
  6. Data show that molecular chaperone BAG6_ubiquitin-like domain (UBL) and ubiquitin-like 4A UBL4A_UBL compete for the same binding site on N-terminal dimerisation domain of SGTA protein (SGTA_NT). PMID: 25415308
  7. This meta-analysis suggested that HLA-B-associated transcript 3 polymorphisms are risk factors for lung cancer. PMID: 25430685
  8. Both TRC35 and Ubl4A have distinct C-terminal binding sites on Bag6 defining a minimal Bag6 complex. PMID: 25535373
  9. Exogenous BAG6 perturbs the function of the BAG6 complex at a stage subsequent to substrate recognition and polyubiquitylation, most likely the BAG6-dependent delivery of OpD to the proteasome. PMID: 24806960
  10. Susceptibility to large-joint osteoarthritis (hip and knee) is associated with BAG6 rs3117582 SNP and the VNTR polymorphism in the second exon of the FAM46A gene on chromosome 6. PMID: 25231575
  11. Tim-3/Gal-9 interaction favors apoptosis of MBP-specific T lymphocytes in benign multiple sclerosis; this process is reduced in PPMS by the up-regulation of Bat3 PMID: 25091272
  12. show that endogenous dislocation clients are captured specifically in association with the cytosolic chaperone BAG6, or retrieved en masse via their glycan handle PMID: 24594942
  13. This meta-analysis suggested that BAT3 polymorphisms contributed the development of lung cancer. PMID: 24989925
  14. RNF126 is recruited to the N-terminal Ubl domain of Bag6 and preferentially ubiquitinates juxtahydrophobic lysine residues on Bag6-associated clients. PMID: 24981174
  15. Bag6, a protein in the TRC pathway that is also responsible for the degradation of mislocalized proteins, is not only involved in core particle assembly but also has a key role in efficient regulatory particle assembly. PMID: 23900548
  16. we show for the first time that BAG-6(686-936) comprises a subdomain of BAG-6, which is sufficient for receptor docking and inhibition of NKp30-dependent NK cell cytotoxicity as part of a tumor immune escape mechanism PMID: 24133212
  17. Data indicate that BCL2-associated athanogene 6 (BAG6) appears to be the central component for the process, as depletion of BAG6 leads to the loss of both UBL4A and GET4 proteins and resistance to cell killing by DNA-damaging agents. PMID: 23723067
  18. Data indicate that the Bag6-Ubl4A-Trc35 complex is localized to the endoplasmic reticulum (ER) membrane to regulate ER-associated degradation (ERAD). PMID: 23665563
  19. our results suggest an essential role for AIF and its binding partner Scythe in the pathway leading to apoptotic corpse clearance. PMID: 23077592
  20. SGTA recognizes a noncanonical ubiquitin-like domain in the Bag6-Ubl4A-Trc35 complex to promote endoplasmic reticulum-associated degradation. PMID: 23246001
  21. Aberrant enhancement of YWK-II/APLP2 by nuclear export of Bat3 may play a role in cancer development by inhibiting cell apoptosis. PMID: 22641691
  22. A BAG6/SGTA cycle operates during protein maturation and quality control in the cytosol. PMID: 23129660
  23. Bat3 promotes T cell responses and autoimmunity by repressing Tim-3-mediated cell death and exhaustion. PMID: 22863785
  24. Cell type-specific subcellular expression of BAT3 suggests distinct functions in the cytosol and in the nucleus. PMID: 22558287
  25. These findings identify a novel role for Bat3 in regulating DOT1L function, which plays a critical role in DNA damage response. PMID: 22373577
  26. Data show that cleavage of Scythe by caspase-3 occurs after activation of both the extrinsic (i.e. Fas/APO-1-mediated) and the intrinsic (i.e. staurosporine-induced) apoptosis pathway. PMID: 22285488
  27. BAT3, a cytosolic chaperone, is recruited to the site of dislocation through its interaction with Derlin2. PMID: 22174835
  28. We present a mechanism explaining how parallel IFNgamma-mediated regulation of CIITA and of its chaperone BAT3 controls the level of components of the HLA class II processing pathway. PMID: 21940994
  29. Data suggest that BAG-6 is necessary for ubiquitin-mediated degradation of newly synthesized defective polypeptides. PMID: 20713601
  30. Evidence indicates that casp3 activated by ricin acts on BAT3 at the caspase cleavage site, DEQD(1001) to release a C-terminal fragment designated CTF-131, which induces phosphatidylserine exposure, cell rounding, and chromatin condensation as ricin does PMID: 14960581
  31. Interaction of hSGT with Hsc70, Hsp70 or Bag-6/Bat-3/Scythe was demonstrated in prometaphase, thereby suggesting a possible role for complexes containing hSGT and distinct (co)-chaperones during mitosis. PMID: 16777091
  32. MRK phosphorylates Scythe at T1080 in vitro as determined by site-directed mutagenesis and mass spectrometry, supporting the consensus and suggesting Scythe as a physiological substrate for MRK. PMID: 16954377
  33. Bat3 is a novel and essential regulator of p53-mediated responses to genotoxic stress, and that Bat3 controls DNA damage-induced acetylation of p53. PMID: 17403783
  34. HLA-B-associated transcript 3 (BAT3) was released from tumor cells, bound directly to NKp30, and engaged NKp30 on NK cells. BAT3 triggered NKp30-mediated cytotoxicity and was necessary for tumor rejection in a multiple myeloma model. PMID: 18055229
  35. Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis PMID: 18056262
  36. BORIS acts as a platform upon which BAT3 and SET1A assemble and exert effects upon chromatin structure and gene expression. PMID: 18765639
  37. NKp30-mediated NK cells/dendritic cells cross talk resulting either in iDC killing or maturation was BAT3-dependent PMID: 18852879
  38. colocalization of PBF and Scythe/BAT3 in the nucleus might be an important factor for survival of osteosarcoma cells. PMID: 19018758
  39. The HSP70-driven degradation of BAG6, following the BAG6-dependent accumulation of HSP70, could allow the protein-refolding activity of HSP70 and limit the extent of its induction. PMID: 19357808
  40. occurrence of an unusual TG 3' splice site in intron 6 PMID: 17672918

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Subcellular Location
Cytoplasm, cytosol. Nucleus. Secreted, extracellular exosome.
Tissue Specificity
Expressed by immature dendritic cells (at protein level).
Database Links

HGNC: 13919

OMIM: 142590

KEGG: hsa:7917

STRING: 9606.ENSP00000365131

UniGene: Hs.440900

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