Human Heparanase,HPA ELISA kit

Instructions
Code CSB-E09899h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name heparanase
Alternative Names HPA, HPR1, HPSE1, HSE1, heparanase exon 9&10 deletion|heparanase exon10-deletion|heparanase-1
Abbreviation HPSE
Uniprot No. Q9Y251
Species Homo sapiens (Human)
Sample Types serum, plasma, ascitic fluid, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-98  
1:2 Average % 97  
Range % 90-105  
1:4 Average % 97  
Range % 92-110  
1:8 Average % 94  
Range % 86-98  
Recovery
The recovery of human HPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 89-110  
EDTA plasma (n=4) 95 90-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.761 2.743 2.752 2.636  
5 2.436 2.424 2.430 2.314  
2.5 1.902 1.915 1.909 1.793  
1.25 1.203 1.219 1.211 1.095  
0.625 0.604 0.612 0.608 0.492  
0.312 0.325 0.320 0.323 0.207  
0.156 0.223 0.231 0.227 0.111  
0 0.112 0.119 0.116    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
Subcellular Location Lysosome membrane, Peripheral membrane protein, Secreted, Nucleus
Protein Families Glycosyl hydrolase 79 family
Tissue Specificity Highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. Also expressed in hair follicles, specifically in both Henle's and Huxley's lay
Database Links

HGNC: 5164

OMIM: 604724

KEGG: hsa:10855

STRING: 9606.ENSP00000308107

UniGene: Hs.44227

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