Human Hyaluronidase-2(HYAL2) ELISA kit

Instructions
Code CSB-EL010919HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name hyaluronoglucosaminidase 2
Alternative Names Hyal 2 ELISA Kit; Hyal-2 ELISA Kit; Hyal2 ELISA Kit; HYAL2_HUMAN ELISA Kit; Hyaluronidase 2 ELISA Kit; Hyaluronidase 2 Precursor ELISA Kit; Hyaluronidase-2 ELISA Kit; Hyaluronoglucosaminidase 2 ELISA Kit; Hyaluronoglucosaminidase-2 ELISA Kit; LUCA 2 ELISA Kit; LUCA-2 ELISA Kit; LUCA2 ELISA Kit; Lung carcinoma protein 2 ELISA Kit; lysosomal hyaluronidase ELISA Kit; PH 20 homolog ELISA Kit; PH-20 homolog ELISA Kit; PH20 homolog ELISA Kit
Abbreviation HYAL2
Uniprot No. Q12891
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HYAL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 89  
Range % 84-93  
1:10 Average % 98  
Range % 84-102  
1:20 Average % 84  
Range % 80-88  
1:40 Average % 95  
Range % 90-100  
Recovery
The recovery of human HYAL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 103 99-108  
EDTA plasma (n=4) 92 88-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.382 2.301 2.342 2.185  
10 1.852 1.802 1.827 1.670  
5 1.170 1.195 1.183 1.026  
2.5 0.789 0.837 0.813 0.656  
1.25 0.471 0.458 0.465 0.308  
0.625 0.335 0.345 0.340 0.183  
0.312 0.227 0.213 0.220 0.063  
0 0.160 0.154 0.157    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R.
Gene References into Functions
  1. an important role for HYAL2 in CD44 alternative splicing. PMID: 29162741
  2. genetic variation in HYAL2 influences platelet aggregation PMID: 28300864
  3. Knocking down HYAL2 in HUVECs protected against HA degradation in the glycocalyx by inhibiting the expression and activity of HYAL2 and further blocked the dephosphorylation of eNOS-Ser-633 and the decrease in NO production in response to LSS. PMID: 27798230
  4. HYAL2 mutations identified as a cause of syndromic orofacial clefting and cor triatriatum sinister in amish families. PMID: 28081210
  5. our data uncover a previously unsuspected mechanism of how hyaluronan and Hyal-2 control platelet generation. PMID: 27398974
  6. Data show that DNA methylation at CpG island of hyaluronoglucosaminidase 2 (HYLA2) could be used to identify stage II and III colon cancer patients who are most likely to benefit from 5-flourouracil chemotherapy with respect to progression-free survival. PMID: 26453961
  7. CD44 knock-down in bovine and human chondrocytes results in release of bound HYAL2. PMID: 25864644
  8. High expression of S100P and HYAL2 is significantly associated with advanced disease and shorter survival of triple-negative breast cancer and S100P and HYAL2 could be potential prognostic markers of TNBC. PMID: 26112095
  9. HYAL2 plays a redundant role in the catalysis of megadalton HA to its 20 kDa intermediate during fertilization. PMID: 25232017
  10. Lower platelet HYAL2 levels and activity are associated with inflammatory bowel disease. PMID: 25411425
  11. A strong association between decreased HYAL2 methylation in peripheral blood and BC. PMID: 25213452
  12. Inverse expression of hyaluronidase 2 and hyaluronan synthases 1-3 is associated with reduced hyaluronan content in malignant cutaneous melanoma. PMID: 23560496
  13. HAS2-HYAL2/CD44 system may support spontaneous chemokinesis of human cancer cells through self-degradation of HMW-HA to produce LMW-HA by an autocrine mechanism. PMID: 21743962
  14. Hyaluronan synthases (HAS1-3) and hyaluronidases (HYAL1-2) in the accumulation of hyaluronan in endometrioid endometrial carcinoma PMID: 20875124
  15. Overexpression of HYAL2 is associated with colorectal cancer. PMID: 20849597
  16. ROS induce Hyal2, suggesting that Hyal2 is likely responsible for the sustained HA fragmentation in the airway lumen observed in inflammatory conditions associated with oxidative stress. PMID: 20554532
  17. Data show that a significant in expression levels of HA synthases (HASs) and hyaluronidases (Hyals) was observed in vitro on stimulation of epithelial ovarian carcinoma cells by gonadotropins. PMID: 20072653
  18. more elevated in human brain metastases than in primary brain tumours PMID: 12174938
  19. suppresses transformation by the Env proteins of jaagsiekte sheep retrovirus and enzootic nasal tumor virus in rodent fibroblasts by increasing Env degradation PMID: 12584308
  20. Hyaluronidase 2 negatively regulates RON receptor tyrosine kinase and mediates transformation of epithelial cells by jaagsiekte sheep retrovirus. PMID: 12676986
  21. Down-regulation of HYAL2 is associated with small cell lung cancer and glioma PMID: 12684632
  22. soluble active form expressed and characterized as Jaagsiekte sheep retrovirus receptor PMID: 15596803
  23. In chondrocytes, HYAL-2 appears to be constitutively expressed and not inducibly regulated by catabolic agents. As such, it appears that the expression of lysosomal hyaluronidase participates little in the overall regulation of hyaluronan catabolism. PMID: 15923194
  24. Association of hyaluronidases 1/2 expresion with neurons in the infarcted and peri-infarcted regions of the middle cerebral artery. PMID: 16837837
  25. purified human Hyal2 is a weak acid-active hyaluronidase PMID: 17229709
  26. alternative splicing variants, M-RIP, HYAL2, CDCA1, and MSMB genes showed differential expressions between cancer cells and corresponding normal tissues. PMID: 19081476
  27. Down-regulation of RBSP3/CTDSPL, NPRL2/G21, RASSF1A, ITGA9, HYAL1 and HYAL2 genes in non-small cell lung cancer PMID: 19140316
  28. HYAL1, but not HYAL2, expression is reduced and correlates with the accumulation of hyaluronan in ovarian carcinomas. PMID: 19435493
  29. platelets and megakaryocytes contain only hyaluronidase 2 (HYAL2) but not HYAL1 PMID: 19443707
  30. Similar in structure to hyaluronidases, which intracellularly degrade hyaluronan. The accumulation of hyaluronan (HA) in the renal cortex is a characteristic feature of inflammatory renal diseases and could participate in immune renal injury. PMID: 9933825

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Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families Glycosyl hydrolase 56 family
Tissue Specificity Widely expressed. No expression detected in adult brain.
Database Links

HGNC: 5321

OMIM: 603551

KEGG: hsa:8692

STRING: 9606.ENSP00000350387

UniGene: Hs.76873

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