Recombinant Human Hyaluronidase-2 (HYAL2)

Code CSB-EP618635HU
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP618635HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP618635HU
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
HYAL2
Uniprot No.
Research Area
Cancer
Alternative Names
Hyal 2; Hyal-2; Hyal2; HYAL2_HUMAN; Hyaluronidase 2; Hyaluronidase 2 Precursor; Hyaluronidase-2; Hyaluronoglucosaminidase 2; Hyaluronoglucosaminidase-2; LUCA 2; LUCA-2; LUCA2; Lung carcinoma protein 2; lysosomal hyaluronidase; PH 20 homolog; PH-20 homolog; PH20 homolog
Species
Homo sapiens (Human)
Expression Region
21-448aa
Target Protein Sequence
MELKPTAPPIFTGRPFVVAWDVPTQDCGPRLKVPLDLNAFDVQASPNEGFVNQNITIFYRDRLGLYPRFDSAGRSVHGGVPQNVSLWAHRKMLQKRVEHYIRTQESAGLAVIDWEDWRPVWVRNWQDKDVYRRLSRQLVASRHPDWPPDRIVKQAQYEFEFAAQQFMLETLRYVKAVRPRHLWGFYLFPDCYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSRHGRNFVSFRVQEALRVARTHHANHALPVYVFTRPTYSRRLTGLSEMDLISTIGESAALGAAGVILWGDAGYTTSTETCQYLKDYLTRLLVPYVVNVSWATQYCSRAQCHGHGRCVRRNPSASTFLHLSTNSFRLVPGHAPGEPQLRPVGELSWADIDHLQTHFRCQCYLGWSGEQCQWDHRQAAGG
Mol. Weight
51.3kD
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R.
Gene References into Functions
  1. an important role for HYAL2 in CD44 alternative splicing. PMID: 29162741
  2. genetic variation in HYAL2 influences platelet aggregation PMID: 28300864
  3. Knocking down HYAL2 in HUVECs protected against HA degradation in the glycocalyx by inhibiting the expression and activity of HYAL2 and further blocked the dephosphorylation of eNOS-Ser-633 and the decrease in NO production in response to LSS. PMID: 27798230
  4. HYAL2 mutations identified as a cause of syndromic orofacial clefting and cor triatriatum sinister in amish families. PMID: 28081210
  5. our data uncover a previously unsuspected mechanism of how hyaluronan and Hyal-2 control platelet generation. PMID: 27398974
  6. Data show that DNA methylation at CpG island of hyaluronoglucosaminidase 2 (HYLA2) could be used to identify stage II and III colon cancer patients who are most likely to benefit from 5-flourouracil chemotherapy with respect to progression-free survival. PMID: 26453961
  7. CD44 knock-down in bovine and human chondrocytes results in release of bound HYAL2. PMID: 25864644
  8. High expression of S100P and HYAL2 is significantly associated with advanced disease and shorter survival of triple-negative breast cancer and S100P and HYAL2 could be potential prognostic markers of TNBC. PMID: 26112095
  9. HYAL2 plays a redundant role in the catalysis of megadalton HA to its 20 kDa intermediate during fertilization. PMID: 25232017
  10. Lower platelet HYAL2 levels and activity are associated with inflammatory bowel disease. PMID: 25411425
  11. A strong association between decreased HYAL2 methylation in peripheral blood and BC. PMID: 25213452
  12. Inverse expression of hyaluronidase 2 and hyaluronan synthases 1-3 is associated with reduced hyaluronan content in malignant cutaneous melanoma. PMID: 23560496
  13. HAS2-HYAL2/CD44 system may support spontaneous chemokinesis of human cancer cells through self-degradation of HMW-HA to produce LMW-HA by an autocrine mechanism. PMID: 21743962
  14. Hyaluronan synthases (HAS1-3) and hyaluronidases (HYAL1-2) in the accumulation of hyaluronan in endometrioid endometrial carcinoma PMID: 20875124
  15. Overexpression of HYAL2 is associated with colorectal cancer. PMID: 20849597
  16. ROS induce Hyal2, suggesting that Hyal2 is likely responsible for the sustained HA fragmentation in the airway lumen observed in inflammatory conditions associated with oxidative stress. PMID: 20554532
  17. Data show that a significant in expression levels of HA synthases (HASs) and hyaluronidases (Hyals) was observed in vitro on stimulation of epithelial ovarian carcinoma cells by gonadotropins. PMID: 20072653
  18. more elevated in human brain metastases than in primary brain tumours PMID: 12174938
  19. suppresses transformation by the Env proteins of jaagsiekte sheep retrovirus and enzootic nasal tumor virus in rodent fibroblasts by increasing Env degradation PMID: 12584308
  20. Hyaluronidase 2 negatively regulates RON receptor tyrosine kinase and mediates transformation of epithelial cells by jaagsiekte sheep retrovirus. PMID: 12676986
  21. Down-regulation of HYAL2 is associated with small cell lung cancer and glioma PMID: 12684632
  22. soluble active form expressed and characterized as Jaagsiekte sheep retrovirus receptor PMID: 15596803
  23. In chondrocytes, HYAL-2 appears to be constitutively expressed and not inducibly regulated by catabolic agents. As such, it appears that the expression of lysosomal hyaluronidase participates little in the overall regulation of hyaluronan catabolism. PMID: 15923194
  24. Association of hyaluronidases 1/2 expresion with neurons in the infarcted and peri-infarcted regions of the middle cerebral artery. PMID: 16837837
  25. purified human Hyal2 is a weak acid-active hyaluronidase PMID: 17229709
  26. alternative splicing variants, M-RIP, HYAL2, CDCA1, and MSMB genes showed differential expressions between cancer cells and corresponding normal tissues. PMID: 19081476
  27. Down-regulation of RBSP3/CTDSPL, NPRL2/G21, RASSF1A, ITGA9, HYAL1 and HYAL2 genes in non-small cell lung cancer PMID: 19140316
  28. HYAL1, but not HYAL2, expression is reduced and correlates with the accumulation of hyaluronan in ovarian carcinomas. PMID: 19435493
  29. platelets and megakaryocytes contain only hyaluronidase 2 (HYAL2) but not HYAL1 PMID: 19443707
  30. Similar in structure to hyaluronidases, which intracellularly degrade hyaluronan. The accumulation of hyaluronan (HA) in the renal cortex is a characteristic feature of inflammatory renal diseases and could participate in immune renal injury. PMID: 9933825

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Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
Glycosyl hydrolase 56 family
Tissue Specificity
Widely expressed. No expression detected in adult brain.
Database Links

HGNC: 5321

OMIM: 603551

KEGG: hsa:8692

STRING: 9606.ENSP00000350387

UniGene: Hs.76873

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