Human Ig kappa chain V-IV region(IGKV4-1) ELISA kit

Human Ig kappa chain V-IV region(IGKV4-1) ELISA kit

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Human Ig kappa chain V-IV region(IGKV4-1) ELISA kit

Protocol MSDS

Code	CSB-EL011443HU
Size	96T,5×96T,10×96T
Price	Request a Quote
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Alternative Names

IGKV4-1Immunoglobulin kappa variable 4-1 ELISA Kit; Ig kappa chain V-IV region B17 ELISA Kit; Ig kappa chain V-IV region JI ELISA Kit; Ig kappa chain V-IV region Len ELISA Kit; Ig kappa chain V-IV region STH ELISA Kit

Abbreviation

IGKV4-1

Uniprot No.

P06312

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates.

Detection Range

0.156 ng/ml -10 ng/ml

Sensitivity

0.039 ng/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGKV4-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	96
	Range %	89-103
1:4	Average %	97
	Range %	92-103
1:8	Average %	93
	Range %	86-100

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.069

2.034

2.051

1.922

1.347

1.367

1.357

1.228

2.5

0.797

0.828

0.812

0.683

1.25

0.428

0.409

0.419

0.289

0.625

0.294

0.311

0.302

0.173

0.312

0.235

0.243

0.239

0.110

0.156

0.157

0.164

0.160

0.031

0.134

0.125

0.130

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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Function
(From Uniprot)

V segment of the variable domain of immunoglobulins light chain that participates in the antigen recognition. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.

Subcellular Location

Secreted. Cell membrane.

Database Links

HGNC: 5834

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.