Human Inhibin A,INH-A ELISA Kit

Instructions
Code CSB-E08237h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name inhibin, alpha
Alternative Names A inhibin subunit ELISA Kit; A inhibin subunit precursor ELISA Kit; AW55578 ELISA Kit; IHA ELISA Kit; inhA ELISA Kit; INHA_HUMAN ELISA Kit; Inhibin alpha chain ELISA Kit; Inhibin alpha chain precursor ELISA Kit; Inhibin alpha subunit ELISA Kit
Abbreviation INHA
Uniprot No. P05111
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.4 pg/mL-80 pg/mL
Sensitivity 0.2 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human INH-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 82-94  
1:2 Average % 94  
Range % 91-98  
1:4 Average % 102  
Range % 98-105  
1:8 Average % 89  
Range % 82-95  
Recovery
The recovery of human INH-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-97  
EDTA plasma (n=4) 101 98-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
80 0.184 0.186 0.185    
20 0.332 0.338 0.335    
6.4 0.541 0.556 0.549    
1.6 0.869 0.897 0.883    
0.4 1.394 1.287 1.341    
0 2.005 2.145 2.075    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

We need dimeric human inhibin A.

A:
Thanks for your inquiry. Based on your information,we have #CSB-E08237h for you.This kit employs the competitive inhibition enzyme immunoassay technique. And it is directed to monomer.Theoretically,this kit detects all the materials that contains monomer.Simply speaking,this kit can detect dimeric human inhibin A. I will send you the kit manual by email.

Target Data

Function Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Gene References into Functions
  1. Inhibin is a novel paracrine factor for tumor angiogenesis and metastasis. PMID: 29535220
  2. HIF1A and EPAS1 potentiate hypoxia-induced upregulation of INHA expression in human term cytotrophoblasts in vitro. PMID: 28115494
  3. There is genetic heterogeneity regarding the INHA gene in different populations, and among the causes of premature ovarian insufficiency. The G769A variant was found in only one patient in the Premature Ovarian Insufficiency Group and in no control, and it appears to be rare in Brazilian patients with premature ovarian insufficiency. PMID: 29091146
  4. Inhibin-alpha gene is transcriptionally silenced in human leukemia cells. In addition, mutations in, and expression levels of, the inhibin-alpha gene differed between human lymphoid and myeloid leukemia cells. PMID: 28260095
  5. Results suggest that high expression levels of alpha-inhibin and beta-glycan transcripts in secretory phase endometrium are associated with a lower chance of achieving pregnancy with in vitro fertilization. PMID: 27628954
  6. Anti-Mullerian hormone (AMH) and inhibin-A (INH-A) levels were found to be significantly higher in the polycystic ovary syndrome (PCOS) group compared to the controls. PMID: 27125339
  7. Immunostaining allowed the identification of specific cell types with E-cadherin, p53, and inhibin-alpha expression in all cases. PMID: 26683836
  8. Each follicular fluid (FF) was individually aspirated and FF/serum EG-VEGF, inhibin-a, and FF IGF-1 levels were evaluated. The pregnant group was characterized by increased numbers of WVFs (p = 0.044), a WVE (p = 0.022), and increased levels of FF IGF-1 (p = 0.001) and serum EG-VEGF (p = 0.03). PMID: 27484063
  9. The Sertoli cell hormones inhibin-B and anti Mullerian hormone have different patterns of secretion in prepubertal cryptorchid boys. PMID: 26452703
  10. Aberrant methylation and common genetic variation in the INHA promoter occur in human adrenocortical carcinoma and are associated with decreased INHA expression. PMID: 25111790
  11. the correlation between placental DNAm of INHBA/FN1 and maternal serum INHA/FN1 levels indicate that DNAm may be a useful tool to identify novel biomarkers for adverse pregnancy outcomes in some cases. PMID: 26654447
  12. The present study did not find an association between INHA-124A>G and -16C>T and the risk of premature ovarian failure (POF). For the 769G>A mutation, Asian women with the 769A allele were found to be susceptible to POF. PMID: 25445105
  13. Inhibin alpha-subunit, Melan A and MNF116 were not sensitive for pheochromocytomas. PMID: 25329118
  14. Inhibin alpha gene expression in human trophoblasts is regulated by interactions between TFAP2 and cAMP signaling pathways. PMID: 25358080
  15. For INHA gene c.-124G>A, males carrying c.-124GG genotype and c.-124GA genotype showed an increased risk of non-normozoospermic syndrome. PMID: 25617520
  16. Maternal serum inhibin-A levels were significantly higher in pregnancies with fetal Hb Bart's disease. PMID: 24107149
  17. Serum inhibin levels correlate with clinical characteristics and mortality in epithelial ovarian cancer patients. PMID: 24599287
  18. When two variant alleles of inhibin alpha gene promoter are combined, the c.-16C>T and c.-124A>G polymorphisms are associated with increased primary ovarian insufficiency risk in Korean women. PMID: 24269065
  19. Data suggest placentation (here, placenta basal plate surface area) and maternal serum PAPP-A (pregnancy-associated plasma protein A) are significantly lower and maternal serum INHA is significantly higher in pre-eclampsia than in control subjects. PMID: 23756051
  20. Data indicate that fallopian tube and the epithelial component of Brenner tumours (BTs) expressed AKR1C3 and androgen receptor, but the tumour stromal cells showed strong expression of calretinin, inhibin and steroidogenic factor 1 in the majority of BTs. PMID: 24012099
  21. Serum inhibin B was significantly higher in breastfed adolescent males than in their peers. PMID: 23836275
  22. Serum inhibin A level might be a useful biomarker for diagnosis and monitoring of preeclampsia PMID: 21535305
  23. located in the syncytiotrophoblast and the intermediate trophoblast of the placenta during early pregnancy; no remarkable findings in placentas of pathological gestations PMID: 23724521
  24. inhibin alpha had a diagnostic role, differentiating between the adrenocortical and adrenomedullary neoplasms. Inhibin/activin beta might play a predictive role for malignant potential in pheochromocytoma. PMID: 22417854
  25. the inhibinalpha gene is transcriptionally silenced in human gastric cancer cells PMID: 22581369
  26. Inhibin alpha subunit staining is not detected in any of the major anatomical regions of the human brain. PMID: 22296042
  27. A subset of primary mixed or acinar and mucin-producing acinar carcinomas (10%) and large-cell carcinomas (22%) expressed inhibin-alpha. PMID: 22585605
  28. Expression of transcription factors controlling alpha inhibin gene expression in placental tissues from pregnancies affected by fetal Down syndrome. PMID: 22430733
  29. This is the first report of immunoreactivity for inhibin-alpha and synaptophysin in synovial sarcoma PMID: 22055401
  30. Human amniotic fluid activin-A and inhibin-A are involved in biological processes linked to intra-amniotic infection/inflammation-induced preterm birth. PMID: 21992678
  31. The inhibin-alpha -16C/T and -124A/G polymorphisms are genetic factors associated with the prevalence of premature ovarian failure in Korean women. PMID: 21785376
  32. Inhibin alpha-subunit N terminus interacts with activin type IB receptor to disrupt activin signaling. PMID: 22267736
  33. GRP94, FN14, and inhibin have roles in brain and non-brain metastases in ErbB-2+ and ErbB-2- breast neoplasms PMID: 21708117
  34. Inhibin pro-alphaC is produced by the adrenal gland. Differentiation between adrenocortical adenoma (ADA) and adrenocortical carcinoma (ACC) by serum inhibin pro-alphaC is limited, but its levels may constitute a novel tumor marker for ACC. PMID: 22127493
  35. Inhibin A enhances bone formation during distraction osteogenesis. PMID: 21809377
  36. Inhibin bodies are a new marker for immature Sertoli cells. PMID: 21707703
  37. study showed that inhibin A was an important predictable marker of preeclampsia PMID: 21252475
  38. Data show that inhibin A levels were significantly lower in the PCOS group compared to the control group, and inhibin B levels were comparable in the two groups. PMID: 21529351
  39. Our data support the hypothesis that elevated second trimester maternal serum levels of total hCG and/or inhibin-A may result from premature accelerated differentiation of the villous cytotrophoblasts. PMID: 21388678
  40. Inhibin-alpha subunit expression in uterine endometrioid adenocarcinomas and endometrial cancer cell lines: a potential prognostic factor. PMID: 21174065
  41. The PAX2(-), PAX8(-), and inhibin A(+) profile supports the diagnosis of hemangioblastoma with a sensitivity of 95%, specificity of 100%, and positive predictive value of 100%. PMID: 21263247
  42. Increase in Inhibin A is associated with breast cancer. PMID: 21115906
  43. analysis of the evolutionary origin of the inhibin alpha-subunit, a unique TGFbeta superfamily antagonist PMID: 20209104
  44. Report serum/synovial fluid activin A and inhibin A levels in rheumatoid arthritis, systemic lupus erythematosus and knee ostoearthritis. PMID: 20704626
  45. in the inhibin alpha gene is strongly associated with premature ovarian failure in Indian patients. PMID: 19324345
  46. The data suggest that, in humans, inhibin is secreted by Sertoli cells into the seminiferous tubules and then travels towards the efferent ducts, where it is reabsorbed into the bloodstream. PMID: 20188029
  47. DNA hypermethylation of the inhibin alpha-subunit gene in prostate carcinoma PMID: 11818495
  48. Patients affected by premature ovarian failure for the missense mutation (769G-->A transition) in the exon 2 of the INHalpha gene. PMID: 12093833
  49. findings suggest that inhibin A is a specific marker of early pregnancy loss before the onset of the clinical symptoms of recurrent miscarriage PMID: 12456605
  50. To determine which cell types in the testes may produce inhibin B, the localization of the two subunits of inhibin B was examined in adult testicular biopsies with normal spermatogenesis, spermatogenic arrest, or Sertoli cell only tubules. PMID: 12493718

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Subcellular Location Secreted
Protein Families TGF-beta family
Tissue Specificity Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal g
Database Links

HGNC: 6065

OMIM: 147380

KEGG: hsa:3623

STRING: 9606.ENSP00000243786

UniGene: Hs.407506

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