Human LIM and SH3 domain protein 1(LASP1) ELISA kit

Instructions
Code CSB-EL012760HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name LIM and SH3 protein 1
Alternative Names LASP 1 ELISA Kit; LASP-1 ELISA Kit; LASP1 ELISA Kit; LASP1_HUMAN ELISA Kit; LIM and SH3 domain protein 1 ELISA Kit; LIM and SH3 protein 1 ELISA Kit; Metastatic lymph node gene 50 protein ELISA Kit; MLN 50 ELISA Kit; MLN50 ELISA Kit; OTTHUMP00000164238 ELISA Kit; OTTHUMP00000164239 ELISA Kit
Abbreviation LASP1
Uniprot No. Q14847
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LASP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %97
Range %92-101
1:2Average %88
Range %83-92
1:4Average %97
Range %93-101
1:8Average %85
Range %80-92
Recovery
The recovery of human LASP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9995-103
EDTA plasma (n=4)9187-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.603 2.572 2.588 2.457
8002.157 2.106 2.132 2.001
4001.640 1.524 1.582 1.451
2001.153 1.069 1.111 0.980
1000.714 0.742 0.728 0.597
500.419 0.443 0.431 0.300
250.249 0.271 0.260 0.129
00.127 0.135 0.131  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).
Gene References into Functions
  1. Data shows that LASP1 is significantly upregulated in breast cancer tissues and cell lines, and its upregulation significantly associated with disease progression. Also, LASP1 was identified as a target gene of miR-133a, and its expression negatively regulated by miR-133a in breast cancer cells. PMID: 29207145
  2. An in vitro study showed that overexpression of miR-133b inhibited the proliferation, migration, and invasion of HepG2 and Hep3B cells. Similarly, knockdown of LASP1 reduced HepG2 and Hep3B cell proliferation, migration, and invasion. PMID: 28117027
  3. Findings indicated that LASP-1, as a downstream target of SOX9, may act as a novel biomarker for lung cancer and plays an important role in cell proliferation, migration, and invasion. PMID: 29138807
  4. miR-203a-3p suppresses tumor growth and metastasis through targeting LASP1 in NPC. PMID: 28982387
  5. LASP1 silencing elicited effects similar to miR-326 overexpression on HCC cells. PMID: 28713953
  6. A combination of low 14-3-3sigma and high LASP1 expression shows a worse trend with overall survival of colorectal cancer patients. PMID: 27156963
  7. Furthermore, we identify flotillin-1 (FLOT1) and histone H1 as downstream factors for cytoplasmic and nuclear pathway of S100A11, which are required for LASP1-S100A11 axis-mediated epithelial-mesenchymal transition and colorectal cancer progression. PMID: 27181092
  8. Overexpression of LASP-1 in thyroid cancer tissues.LASP-1 role in thyroid cancer cell proliferation and invasion.LASP-1 is an oncogene. PMID: 27938497
  9. Study found LASP1 to be downregulated after knockdown of PVT1 and overexpression of LASP1 attenuated the tumor-suppressive roles of PVT1 knockdown in esophageal squamous cell carcinoma. PMID: 28404954
  10. Data indicate that LIM and SH3 protein 1 (LASP1) is a direct target of miR-145. PMID: 27626692
  11. LASP1 enhances the expression and secretion of MMPs, favoring breast cancer cell migration and invasiveness. PMID: 27588391
  12. miR-218 contributes to PE by targeting LASP1 to inhibit trophoblast invasion PMID: 28412444
  13. The correlation between LIM and SH3 domain-containing protein expression levels in cancer and the poor outcome and metastatic behavior of tumors denotes the clinical significance of this protein and hints its potential value as a new cancer prognostic or even diagnostic biomarker PMID: 28621232
  14. LASP-1 contributes to the formation and progression of prostate cancer through a NF-kappaappa B pathway. PMID: 27840958
  15. High expression of miR218 is associated with gastric cancer. PMID: 27696291
  16. LASP-1 may essentially involve in the metastasis and growth of CCA and clinical significance of LASP-1 may reside in function as a biomarker to predict prognosis and as a promising therapeutic strategy for CCA patients by the inhibition of LASP-1 expression PMID: 26729195
  17. It is a target gene of miR-1. PMID: 26414725
  18. LASP-1 associated with UHRF1, G9a, Snail1 and di- and tri-methylated histoneH3 in a CXCL12-dependent manner based on immunoprecipitation and proximity ligation assays PMID: 25982273
  19. LASP-1 induces proliferation, metastasis and cell cycle arrest at the G2/M phase in gallbladder cancer by down-regulating S100P via the PI3K/AKT pathway. PMID: 26797416
  20. LASP-1, mediated by miR-203, has crucial functions in the proliferation, migration and invasion of human non-small cell lung cancer PMID: 26683818
  21. Detecting an affection of migratory processes after LASP-1 silencing, we propose that LASP-1 could impact on metastasis of CC [choriocarcinoma]cells. PMID: 26232936
  22. Results identified LASP1 as a hitherto unknown protein in melanocytes and as novel partner of dynamin in the physiological process of membrane constriction and melanosome vesicle release. PMID: 26061439
  23. Our results suggest that LASP-1 mRNA overexpression may be mainly implicated in female hepatocellular carcinoma (HCC) and cirrhotic HCCs; and that LASP1 may play its role with vimentin in HCC cells. PMID: 25760690
  24. LASP-1 overexpression was associated with aggressive phenotype in clear cell renal cell cancer. PMID: 24955835
  25. LASP1 plays key roles in cell structure, physiological processes, and cell signaling; overexpression contributes to cancer aggressiveness [review] PMID: 25622104
  26. LIM and SH3 protein 1 induces TGFbeta-mediated epithelial-mesenchymal transition in human colorectal cancer by regulating S100A4 expression. PMID: 25252758
  27. Study revealed that LASP1 phosphorylation results in an association with CRKL - another specific BCR-ABL substrate and bona fide biomarker for BCR-ABL activity. PMID: 24913448
  28. Results defined LASP1 as a direct target gene for HIF1alpha upregulation that is critical for metastatic progression of PDAC. PMID: 25385028
  29. LASP-1, overexpressed in gastric cancer and associated with poor prognosis, plays an important role in the growth and metastasis of gastric cancer. PMID: 24990592
  30. Results show significant upregulation of LASP1 and SCAD protein levels in acute psychotic bipolar disorder samples. PMID: 24554194
  31. LASP1 was a direct target of miR-218 in prostate cells. PMID: 24815849
  32. LASP-1 is linked closely to tumourigenicity in oral cancer. PMID: 24386158
  33. Data indicate decreased cell migratory potential accompanied by enhanced cell adhesion, but no significant inhibition of cell proliferation as measured by in T24 cells upon LIM and SH3 (LASP)-1 protein (LASP-1) knockdown. PMID: 22481019
  34. LASP1 may play an important role in the pathogenesis of esophageal squamous cell carcinoma. PMID: 23254782
  35. Data show that miR-133a can target the 3' untranslated region (3'UTR) of LIM and SH3 protein 1 (LASP1) mRNA and suppress the expression of LASP1. PMID: 23968734
  36. Phosphorylation of LASP-1 by PKA at serine 146 induces translocation of the LASP-1/ZO-2 complex from the cytoplasm to the nucleus. PMID: 22665060
  37. A single-nucleotide polymorphism in the LASP1 gene promoter region is associated with schizophrenia susceptibility. PMID: 23040864
  38. High cytosolic LASP-1 expression is associated with hepatocellular carcinoma. PMID: 23084841
  39. miR-203 inhibits the migration and invasion of esophageal squamous cell carcinoma by regulating LASP1. PMID: 22940702
  40. Up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor in triple negative breast cancer. PMID: 22713668
  41. These results demonstrated that hepatitis B virus X protein could upregulate LASP-1 through PI3-K pathway to promote the proliferation and migration of hepatoma cells. PMID: 22897902
  42. Data indicate that LASP1 may have an oncogenic function and that it might be regulated by miR-1, miR-133a, and miR-218, which may function as tumor suppressive miRNAs in bladder cancer (BC). PMID: 20843712
  43. Data indicate that Lasp-1 is a novel component of podosomes and is involved in the regulation of podosomal function. PMID: 22514729
  44. LASP-1 has an essential role in growth and migration of ovarian carcinoma cells, possibly by influencing the localization of zyxin. PMID: 17211471
  45. LASP-1 gene expression enhances proliferation of colorectal cancer cells and may serve as a useful marker for colorectal cancer progression. PMID: 21215099
  46. LASP-1 interaction with CXCR2 is critical for CXCR2-mediated chemotaxis PMID: 20419088
  47. LASP1 knockdown by small interfering RNA-mediated silencing indicates its funcion role in progression and metastasis of medulloblastoma. PMID: 20924110
  48. LASP-1, S100A9 and RhoGDI were detected by proteomic analysis to be differentially expressed between normal mucosa, non-metastatic colorectal carcinoma and metastatic CRC tissue PMID: 20812987
  49. Overexpression of LASP-1 was found in metastatic colorectal cancer (CRC) tissues (p=0.002), and its expression level was closely correlated with overall survival of patients with CRC (p=0.002). PMID: 20660701
  50. Nuclear LASP-1-positivity may serve as a negative prognostic indicator for long-term survival of breast cancer patients. PMID: 20461080
  51. phosphorylation of LASP by cGK and cAK may be involved in cytoskeletal organization and cell motility PMID: 12571245
  52. LIM-nebulette, Lasp-1, and zyxin may play an important role in the organization of focal adhesions PMID: 15004028
  53. Data suggest that there are no functional differences between human and mouse LASP-1. PMID: 15465019
  54. LASP-1 has an essential role in tumor cell growth and migration, possibly by influencing the localization of zyxin. PMID: 16430883
  55. LASP-1 may have roles in progression of human breast carcinoma PMID: 17956604
  56. p53 may play a role in influencing tumor metastasis through Lasp1 in hepatocellular caarcinoma. PMID: 19155088
  57. The ectopic expression of LASP-1 and uPA supported the proteomic results and showed that uPA up-regulation increased LASP-1 expression and that both were implicated in SKHep1C3 motility. PMID: 19177205
  58. LASP-1 translocation to the cytoskeleton of activated platelets correlates with LASP-1 phosphorylation at tyrosine 171 by Src-kinase PMID: 19718473
  59. In rabbits, lasp-1 phosphorylation is increased by histamine H2 receptor activation and elevation of intracellular cAMP PMID: 9688835
  60. In rabbits, lasp-1 localizes to sites of dynamic actin assembly, identifies sites that are phosphorylated by protein kinase A in vivo, and shows that the phosphorylation of these sites decreases F-actin-binding to lasp-1. PMID: 12432067

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Subcellular Location Cytoplasm, cell cortex, Cytoplasm, cytoskeleton
Database Links

HGNC: 6513

OMIM: 602920

KEGG: hsa:3927

STRING: 9606.ENSP00000325240

UniGene: Hs.741156

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