Human Lecithin Cholesterol Acyltransferase(LCAT) ELISA Kit

Code CSB-E13469h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P04180
Lead Time 3-5 working days
Protein Biological Process 2 Lipogenesis and lipometabolism
Abbreviation LCAT
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name lecithin-cholesterol acyltransferase
Species Homo sapiens (Human)
Protein Biological Process 3 Cholesterol metabolism
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency.
HGNC 6522
RGD 2993
MGI 96755
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LCAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 87  
Range % 82-95  
1:400 Average % 97  
Range % 90-105  
1:800 Average % 101  
Range % 92-110  
1:1600 Average % 92  
Range % 84-98  
Recovery
The recovery of human LCAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 80-98  
EDTA plasma (n=4) 95 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml. OD1 OD2 Average Corrected  
200 2.842 2.752 2.797 2.701  
100 2.247 2.176 2.212 2.116  
50 1.739 1.604 1.672 1.576  
25 1.215 1.139 1.177 1.081  
12.5 0.822 0.804 0.813 0.717  
6.25 0.516 0.499 0.508 0.412  
3.12 0.233 0.229 0.231 0.135  
0 0.097 0.095 0.096    
References
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Function Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs)
Involvement in disease Lecithin-cholesterol acyltransferase deficiency (LCATD); Fish-eye disease (FED)
Subcellular Location Secreted
Protein Families AB hydrolase superfamily, Lipase family
Tissue Specificity Detected in blood plasma (PubMed:3458198, PubMed:8820107, PubMed:10222237). Detected in cerebral spinal fluid (at protein level) (PubMed:10222237). Detected in liver (PubMed:3797244, PubMed:3458198). Expressed mainly in brain, liver and testes.
Database Links

HGNC: 6522

OMIM: 136120

KEGG: hsa:3931

STRING: 9606.ENSP00000264005

UniGene: Hs.387239

Pathway Lipids and Inflammation in Atherogenesis
Inflammasome Signaling
Cholesterol metabolism

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