Human Leucine-rich repeat-containing protein 17(LRRC17) ELISA kit

Code CSB-EL013112HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
leucine rich repeat containing 17
Alternative Names
LRRC17 ELISA Kit; P37NB ELISA Kit; UNQ3076/PRO9909 ELISA Kit; Leucine-rich repeat-containing protein 17 ELISA Kit; p37NB ELISA Kit
Abbreviation
LRRC17
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
23.44 pg/mL-1500 pg/mL
Sensitivity
5.86 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LRRC17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-101
1:2Average %92
Range %85-98
1:4Average %86
Range %80-92
1:8Average %94
Range %90-100
Recovery
The recovery of human LRRC17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-99
EDTA plasma (n=4)9591-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15001.711 1.672 1.692 1.562
7501.426 1.396 1.411 1.281
3751.012 1.004 1.008 0.878
187.50.775 0.756 0.766 0.636
93.750.583 0.544 0.564 0.434
46.870.374 0.359 0.367 0.237
23.440.262 0.247 0.255 0.125
00.133 0.127 0.130  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human LRRC17 ELISA Kit was designed for the quantitative measurement of Human LRRC17 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

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Target Background

Function
(From Uniprot)
Involved in bone homeostasis. Acts as a negative regulator of RANKL-induced osteoclast precursor differentiation from bone marrow precursors.
Gene References into Functions
  1. low circulating plasma level of LRRc17 might serve as an independent and additive risk factor for osteoporotic fractures. PMID: 27355564
  2. Whole-genome microarray mRNA-expression profiles of myofibroblasts and skin fibroblasts revealed four additional genes that are significantly differentially expressed in these two cell types: NKX2-3 and LRRC17 in myofibroblasts and SHOX2 and TBX5 in skin fibroblasts PMID: 27036009
Subcellular Location
Secreted, extracellular space.
Tissue Specificity
Expressed in osteoblast cell lines. Well expressed in ovary, heart, pancreas, skeletal muscle, lung, and fetal kidney and lung and only at the basal levels in the other tissues examined including adult kidney. More expressed in S-type neuroblastoma cells
Database Links

HGNC: 16895

KEGG: hsa:10234

STRING: 9606.ENSP00000344242

UniGene: Hs.567412

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