Human Loricrin(LOR) ELISA kit

Code CSB-EL013037HU
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
loricrin
Alternative Names
LOR ELISA Kit; LOR protein ELISA Kit; LORI_HUMAN ELISA Kit; Loricrin ELISA Kit; LRN ELISA Kit; MGC111513 ELISA Kit; OTTHUMP00000015823 ELISA Kit
Abbreviation
LOR
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
25 pg/mL-1600 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LOR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-89  
1:2 Average % 97  
Range % 94-100  
1:4 Average % 98  
Range % 95-101  
1:8 Average % 87  
Range % 82-91  
Recovery
The recovery of human LOR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-96  
EDTA plasma (n=4) 102 98-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1600 2.424 2.468 2.446 2.300  
800 1.561 1.598 1.580 1.434  
400 0.986 0.946 0.966 0.820  
200 0.634 0.657 0.646 0.500  
100 0.411 0.435 0.423 0.277  
50 0.295 0.275 0.285 0.139  
25 0.202 0.213 0.208 0.062  
0 0.145 0.147 0.146    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human LOR ELISA Kit was designed for the quantitative measurement of Human LOR protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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Target Background

Function
(From Uniprot)
Major keratinocyte cell envelope protein.
Gene References into Functions
  1. Authors report a multi-generation family with prominent ichthyosis and palmoplantar involvement due to a novel mutation in loricrin. PMID: 25965869
  2. Letter: Knockdown of either filaggrin or loricrin increases the productions of interleukin (IL)-1alpha, IL-8, IL-18 and granulocyte macrophage colony-stimulating factor in stratified human keratinocytes. PMID: 26381575
  3. Studies on human keratinocytes recognized that loricrin expression was inversely related to the expression of the cyclin-dependent kinase inhibitor p21 PMID: 25896246
  4. two novel heterozygous frameshift mutations in exon 2 - c.646_647insGCAGCAGGTC, p.Gln216Argfs*123 and c.798_799dupT, p.Gly267Trpfs*69 in loricrin keratoderma patients PMID: 25234742
  5. Results describe a novel frameshift mutation leading to loricrin keratoderma presenting with colloidion membrane PMID: 25142840
  6. We found no mutations of Loricrin in two Progressive symmetrical erythrokeratoderma families. PMID: 23678955
  7. We describe a young man who was a collodion baby and had the typical presentation of Loricrin keratoderma. Direct DNA sequencing identified a heterozygous mutation in the loricrin gene with a single G insertion, 730insG, present in (a) the patient PMID: 22831754
  8. There were no mutations found in the LOR gene and the true pathogenesis of progressive symmetrical erythrokeratodermia remains unknown. PMID: 21198793
  9. These findings suggest that inverse effects of PKCdelta and PKCeta on loricrin expression attributes to the expression of c-Jun and JunD. PMID: 20184865
  10. regulation of gene expression requires interactions among multiple transcription factors in keratinocytes located in different compartments of the epidermis PMID: 12200429
  11. Unique mutations in glycine-rich domain of mutant loricrin form arginine-rich nuclear localization sequences that disrupt differentiation of keratinocytes. PMID: 12615358
  12. Barrier abnormality in loricrin keratoderma is linked to defective CE scaffold, resulting in increased extracellular permeability. PMID: 15102081
  13. ruled out as a candidate for the PSORS4 locus. PMID: 15598222
  14. report the clinical and molecular characterization of a new family with the recurrent 730insG LOR mutation, giving new insights in LK genotype-phenotype correlation PMID: 17953701
  15. results give evidence that heterogeneous phenotypes of LK may be the result of genetic heterogeneity of loricrin mutations, and demonstrate that nuclear accumulation of mutant loricrin is due to the nuclear targeting sequences in the mutant C-terminus. PMID: 18844868
  16. the deregulated increase in SPRR1A expression in chronic atopic skin lesions reflects an insufficient rise in SPRR transcripts, unable to compensate for the lack of LOR and thus contributing to the persistence of chronic atopic dermatitis skin lesions. PMID: 19672094

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Involvement in disease
Vohwinkel syndrome with ichthyosis (VSI)
Subcellular Location
Cytoplasm. Nucleus, nucleoplasm.
Database Links

HGNC: 6663

OMIM: 152445

KEGG: hsa:4014

STRING: 9606.ENSP00000357731

UniGene: Hs.251680

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