Human Ly6/PLAUR domain-containing protein 1(LYPD1) ELISA kit

Code CSB-EL013261HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
LY6/PLAUR domain containing 1
Alternative Names
FLJ41033 ELISA Kit; LY6/PLAUR domain containing 1 ELISA Kit; Ly6/PLAUR domain-containing protein 1 ELISA Kit; Lypd1 ELISA Kit; LYPD1_HUMAN ELISA Kit; LYPDC1 ELISA Kit; MGC29643 ELISA Kit; PHTS ELISA Kit; Putative HeLa tumor suppressor ELISA Kit
Abbreviation
LYPD1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
25 pg/mL-1600 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LYPD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %83-91
1:2Average %97
Range %92-104
1:4Average %85
Range %82-90
1:8Average %93
Range %90-96
Recovery
The recovery of human LYPD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)9892-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.243 2.230 2.237 2.100
8001.485 1.472 1.479 1.342
4000.924 0.984 0.954 0.817
2000.591 0.617 0.604 0.467
1000.433 0.426 0.430 0.293
500.337 0.317 0.327 0.190
250.229 0.237 0.233 0.096
00.136 0.138 0.137
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human LYPD1 ELISA Kit was designed for the quantitative measurement of Human LYPD1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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Target Background

Function
(From Uniprot)
Believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro increases receptor desensitization and decreases affinity for ACh of alpha-4:beta-2-containing nAChRs. May play a role in the intracellular trafficking of alpha-4:beta-2 and alpha-7-containing nAChRs and may inhibit their expression at the cell surface. May be involved in the control of anxiety.
Gene References into Functions
  1. The PHTS is involved in HeLaHF reversion independently of the p53 pathway, and Expression profiling revealed that PHTS is one of the genes that is up-regulated in HeLaHF but not in HeLa. PMID: 16413018
Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Database Links

HGNC: 28431

OMIM: 610450

KEGG: hsa:116372

STRING: 9606.ENSP00000380605

UniGene: Hs.432395

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