Human Lysosomal protective protein(CTSA) ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

cathepsin A

Alternative Names

beta galactosidase 2 ELISA Kit; BETA GALACTOSIDASE PROTECTIVE PROTEIN ELISA Kit; beta-galactosidase 2 ELISA Kit; beta-galactosidase protective protein ELISA Kit; betagalactosidase 2 ELISA Kit; Carboxypeptidase C ELISA Kit; Carboxypeptidase L ELISA Kit; carboxypeptidase Y-like kininase ELISA Kit; Cathepsin A ELISA Kit; Ctsa ELISA Kit; deamidase ELISA Kit; EC 3.4.16.5 ELISA Kit; Glactosialidosis ELISA Kit; GLB2 ELISA Kit; Goldberg Syndrome ELISA Kit; GSL ELISA Kit; lysosomal carboxypeptidase A ELISA Kit; Lysosomal protective protein 20 kDa chain ELISA Kit; Lysosomal protective protein ELISA Kit; Lysosomal protective protein deficiency ELISA Kit; NEURAMINIDASE BETA GALACTOSIDASE EXPRESSION; NGBE ELISA Kit; Neuraminidase deficiency with beta-galactosidase deficiency ELISA Kit; NGBE ELISA Kit; OTTHUMP00000031778 ELISA Kit; OTTHUMP00000031781 ELISA Kit; PPCA ELISA Kit; PPCA deficiency ELISA Kit; PPGB ELISA Kit; PPGB_HUMAN ELISA Kit; Protective protein cathepsin A ELISA Kit; Protective protein for beta galactosidase ELISA Kit; Protective protein for beta-galactosidase ELISA Kit; Protective protein/cathepsin A deficiency ELISA Kit; urinary kininase ELISA Kit

Abbreviation

CTSA

Uniprot No.

P10619

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CTSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	89
	Range %	85-93
1:4	Average %	106
	Range %	102-109
1:8	Average %	94
	Range %	87-98

Recovery

The recovery of human CTSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	96	92-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.745	2.849	2.797	2.693
500	2.257	2.412	2.335	2.231
250	1.616	1.717	1.667	1.563
125	0.943	0.965	0.954	0.850
62.5	0.547	0.524	0.536	0.432
31.2	0.354	0.363	0.359	0.255
15.6	0.245	0.254	0.250	0.146
0	0.103	0.105	0.104

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human CTSA ELISA Kit was designed for the quantitative measurement of Human CTSA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Background

Function
(From Uniprot)

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.

Gene References into Functions

The usefulness of modified U1 snRNA for rescue from exon 7 skipping caused by the IVS7 +3a>g mutation of the CTSA gene. PMID: 30010039
The gene signature of OPA1, CTSA, NDUFA1, STK10 and PRDX1 was able to identify patients post-implant with a sensitivity of 91% and a specificity of 86% in discrimination between post-implant group and healthy controls. PMID: 27177495
Galactosialidosis is a rare lysosomal storage disease caused by a combined deficiency of GM1 beta-galactosidase (beta-gal) and neuraminidase secondary to a defect of a lysosomal enzyme protective protein/cathepsin A (PPCA) and mutation in CTSA gene. PMID: 26259553
Case Report: galactosialidosis with novel mutations of CTSA gene diagnosed using placental pathology. PMID: 25075748
We identified compound heterozygous mutations in the CTSA gene, responsible for causing galactosialidosis PMID: 24769197
correct nomenclature of mutations for this gene is discussed; clinical and mutational analyses of 4 cases with rare infantile form of galactosialidosis; identified 3 novel nucleotide changes, 2 resulting in missense mutations and the third, resulting in the p.Gln406* stop codon; complexity of the clinical phenotypes in GS reflects dual functions of PPCA/CTSA PMID: 23915561
Catalytic function, tissue distribution and substrates of cathepsin A are discussed as well as inhibition of cathepsin A as an emerging strategy for the treatment of heart failure. PMID: 23495688
The Cathepsin C releases the glycosidases from complexes formed with cathepsin A, and reinstates their activity. PMID: 22532132
Our data suggest that CatA is involved in the C-terminal fine-tuning of antigenic T cell epitopes in human APC. PMID: 19954752
mutations in early infantile galactosialidosis in two Dutch patients PMID: 12649068
Increased activity of beta-galactosidase in the peritoneal fluid is associated with gynecologic cancers and pelvic inflammatory disease PMID: 15785934
effects of GLB1, PPCA and NEU1 gene mutations on elastogenesis in skin fibroblasts PMID: 16538002
Results describe the hydrodynamic properties of PPCA, NEU1, and a complex of the two proteins and identified multiple binding sites on both proteins. PMID: 19666471

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Involvement in disease

Galactosialidosis (GSL)

Subcellular Location

Lysosome.

Protein Families

Peptidase S10 family

Database Links

HGNC: 9251

OMIM: 256540

KEGG: hsa:5476

STRING: 9606.ENSP00000361562

UniGene: Hs.609336

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Code	CSB-EL006184HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.