Human Lysyl oxidase homolog 3(LOXL3) ELISA kit

Instructions
Code CSB-EL013042HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lysyl oxidase-like 3
Alternative Names LOXL ELISA Kit; Loxl3 ELISA Kit; LOXL3_HUMAN ELISA Kit; Lysyl oxidase homolog 3 ELISA Kit; Lysyl oxidase like 3 ELISA Kit; Lysyl oxidase-like protein 3 ELISA Kit
Abbreviation LOXL3
Uniprot No. P58215
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LOXL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %89-99
1:2Average %90
Range %84-100
1:4Average %93
Range %89-102
1:8Average %92
Range %84-98
Recovery
The recovery of human LOXL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9586-100
EDTA plasma (n=4)9691-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.299 2.308 2.304 2.099
10001.611 1.634 1.623 1.418
5001.137 1.092 1.115 0.910
2500.691 0.706 0.699 0.494
1250.499 0.507 0.503 0.298
62.50.353 0.365 0.359 0.154
31.250.279 0.267 0.273 0.068
00.202 0.208 0.205
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Protein-lysine 6-oxidase that mediates the oxidation of peptidyl lysine residues to allysine in target proteins
Gene References into Functions
  1. Missense variant in LOXL3 gene is associated with nonsyndromic cleft palate. PMID: 29802726
  2. By deacetylating and deacetyliminating Stat3 on multiple acetyl-lysine sites in nuclei, Loxl3 regulates Stat3 dimerization and transcriptional activity. CD4+ T cell differentiation in inflammatory responses is regulated by the Loxl3-Stat3 signaling pathway PMID: 28065600
  3. Our results suggest that null mutations in LOXL3 are likely associated with autosomal recessive early-onset high myopia. LOXL3 is a potential candidate gene for high myopia, but this possibility should be confirmed in additional studies. PMID: 26957899
  4. LOXL3 is a novel candidate gene for autosomal recessive Stickler syndrome. PMID: 25663169
  5. The human lysyl oxidase-like 3 gene does not confer increased genotypic risk for adolescent idiopathic scoliosis. PMID: 21740577
  6. Lysyl-oxidase-like 3 interacts and cooperates with Snail to downregulate E-cadherin expression, and control epithelial-mesenchymal transitions and carcinoma progression. PMID: 16096638
  7. LOXL3 encodes two variants, LOXL3 and LOXL3-sv1, both of which function as amine oxidases with distinct tissue and substrate specificities from one another. PMID: 17018530
  8. Breast carcinoma effusions showed significantly higher LOXL2 and lower LOXL3 expression compared to primary carcinomas. PMID: 19015874
  9. Expression of LOXL, LOXL2, LOXL3 and LOXL4 was not statistically associated with tumor location, stage, growth type, or differentiation status in colorectal adenocarcinomas PMID: 19724858

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Involvement in disease Defects in LOXL3 are found in a family with an autosomal recessive form of Stickler syndrome, an inherited disorder that associates ocular signs with more or less complete forms of Pierre Robin sequence and sensorineural deafness (PubMed:25663169). Pierre Robin sequence includes an opening in the roof of the mouth (a cleft palate) (PubMed:25663169). The degree of hearing loss varies among affected individuals and may become more severe over time (PubMed:25663169). Syndrome expressivity is variable (PubMed:25663169). Ocular disorders include non-progressive myopia with associated chorioretinal degeneration (PubMed:25663169). Defects in LOXL3 are found in another family with early-onset high myopia (PubMed:26957899). The disease may be caused by mutations affecting the gene represented in this entry (PubMed:25663169, PubMed:26957899).
Subcellular Location Secreted, extracellular space, Cytoplasm, Nucleus
Protein Families Lysyl oxidase family
Tissue Specificity Isoform 1: Predominantly detected in the heart, placenta, lung, and small intestine (PubMed:17018530). Isoform 2: Highly detected in the kidney, pancreas, spleen, and thymus, and is absent in lung (PubMed:17018530). In eye, present in all layers of cornea
Database Links

HGNC: 13869

OMIM: 607163

KEGG: hsa:84695

STRING: 9606.ENSP00000264094

UniGene: Hs.469045

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