Human Metastasis-suppressor KiSS-1(KISS1) ELISA kit

Instructions
Code CSB-EL012373HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name KiSS-1 metastasis-suppressor
Alternative Names KISS 1 ELISA Kit; KiSS 1 metastasis suppressor ELISA Kit; Kiss1 ELISA Kit; KISS1_HUMAN ELISA Kit; Kisspeptin 1 ELISA Kit; Kisspeptin 10 ELISA Kit; Kisspeptin 13 ELISA Kit; Kisspeptin 14 ELISA Kit; Kisspeptin ELISA Kit; Kisspeptin-1 ELISA Kit; Kisspeptin-10 ELISA Kit; Kisspeptin-54 ELISA Kit; Kisspeptin1 ELISA Kit; Malignant melanoma metastasis suppressor ELISA Kit; Metastasis suppressor KiSS 1 ELISA Kit; Metastasis suppressor KiSS1 ELISA Kit; Metastin ELISA Kit; MGC39258 ELISA Kit
Abbreviation KISS1
Uniprot No. Q15726
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human KISS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-94  
1:2 Average % 101  
Range % 97-103  
1:4 Average % 90  
Range % 85-96  
1:8 Average % 95  
Range % 91-100  
Recovery
The recovery of human KISS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 80-93  
EDTA plasma (n=4) 96 92-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.842 2.963 2.903 2.775  
10 2.187 2.203 2.195 2.067  
5 1.148 1.226 1.187 1.059  
2.5 0.755 0.743 0.749 0.621  
1.25 0.349 0.365 0.357 0.229  
0.625 0.222 0.205 0.214 0.086  
0.312 0.202 0.200 0.201 0.073  
0 0.132 0.124 0.128    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Q&A and Customer Reviews

 Q&A
Q:

Which molecule (peptides: kp-54, kp-10, kp-13, kp14) is detected in this kit?

A:
Thanks for your inquiry!Metastasis-suppressor KiSS-1 can be cleaved into kp-54, kp-10, kp-13, kp14. Pls kindly refer to: https://www.uniprot.org/uniprot/Q15726
CSB-EL012373HU
This kit is designed against full length protein. Sorry we are not sure if it can detect the forms you mentioned because we didn't do epitope mapping before.
Pls let me know if you have any further questions. Thank you.

Target Data

Function Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
Gene References into Functions
  1. Expression levels of MACC1, CD44, Twist1, and KiSS-1 are related to duration of overall survival among patients with colonic adenocarcinoma. PMID: 30021598
  2. Data suggest KISS1 as a great candidate for Cervical cancer (CC) molecular markers or as a therapeutic target for CC. Also, HPV presence does not seem to alter the KISS1 expression in CC PMID: 29914007
  3. KiSS-1 metastasis-suppressor protein (KISS1) expression promotes secretion of proangiogenic biomarkers and factors modulating anti-cancer immune responses. PMID: 29269086
  4. The genetic variation of KISS1 gene may be a factor contributing to polycystic ovary syndrome development. PMID: 29848339
  5. Kisspeptin and spexin serum levels in women were negatively correlated with obesity and insulin resistance. PMID: 29137471
  6. Data suggest that women with PCOS (polycystic ovary syndrome) exhibit increased serum kisspeptin-1 levels. PMID: 28933574
  7. Our studies revealed that KISS1 inhibits glycolysis and carbon sources for endogenous fatty acid oxidation (FAO). PMID: 28597070
  8. KiSS1 gene is a novel mediator of TGFbeta-mediated cell invasion in triple negative breast cancer. PMID: 28988968
  9. Kisspeptin signaling modulates steroid biosynthesis in Leydig cells. Kisspeptin produced by the interstitium targets spermatogonia and sperm cells to affect spermatogenesis onset/progression and sperm functions, respectively. PMID: 28878091
  10. Kisspeptin inhibited tumor growth through an EIF2AK2dependent mechanism, and an in vivo metastasis assay identified kisspeptinactivated EIF2AK2 signaling as critical for the suppression of distant metastasis. PMID: 28944853
  11. kisspeptin surge in serum and urine may be used as a marker for dominant follicle development and pre-ovulation. PMID: 28266227
  12. High plasma kisspeptin-1 is associated with premature thelarche. PMID: 28422705
  13. expression appears to be up-regulated in adenomyotic as compared with eutopic glandular endometrium of patients with, as well as women without, adenomyosis PMID: 27940396
  14. Data suggest that subjects with anorexia nervosa display broad spectrum of physical activity (2479-26,047 steps/day) which shows a negative correlation with plasma kisspeptin levels and a positive association with plasma ghrelin levels. PMID: 27693487
  15. Kisspeptin-10 may contribute to accelerate the progression and instability of atheromatous plaques, leading to plaque rupture. The GPR54 antagonist may be useful for prevention and treatment of atherosclerosis. PMID: 28411243
  16. Kiss-10 levels are significantly altered by malignancy and tumour subtypes even in patients with small renal tumours PMID: 28095383
  17. Study compared kisspeptin-54 and kisspeptin-10 to gain an insight into why KP-54 elicits more sustained responses than KP-10 after systemic delivery PMID: 28464043
  18. we conclude that KISS-1 can inhibit the proliferation of osteosarcoma in vitro by accelerating the processes of apoptosis and autophagy. PMID: 28075440
  19. These findings suggest that infundibular kisspeptin neurons are sensitive to circulating sex steroid hormones throughout life and that the sex reversal observed in MTF transsexuals might reflect, at least partially, an atypical brain sexual differentiation. PMID: 27046106
  20. Patients with ovarian epithelial cancer and low KISS1 mRNA expression had shorter survival time than those with high expression (P = 0.001).Preoperative KISS1 mRNA was a potential prognostic biomarker for EOC, and high preoperative KISS1 expression indicated a favorable prognosis. PMID: 27861355
  21. Lower expression of KiSS1 was observed in metastatic Breast Cancer. PMID: 27221854
  22. There is an increasing appreciation that kisspeptin may act as a signal transmitter between metabolic status and reproductive function PMID: 26605678
  23. In human cumulus GCs, kiss1r mRNA levels were positively correlated with age but not with BMI. There was no expression of kiss1 mRNA in either cumulus or mural GCs PMID: 26879207
  24. There was a statistically significant increase in serum kisspeptin levels from the early follicular to the preovulatory phase, and from the preovulatory to the luteal phase. PMID: 25968289
  25. Overexpression of KiSS-1 suppressed the invasiveness of CRC cells, and the gene exerted its function by reducing the expression of MMP-9 via blocking of tge PI3K/Akt/NF-kappaB pathway. PMID: 26847533
  26. kisspeptins play essential roles in reproduction [review] PMID: 26702158
  27. Increased placental kisspeptin expression is consistent with reduced trophoblast invasiveness and may represent a molecular mechanism that explains the development of preeclampsia. Decreased circulating kisspeptin concentration has the potential to be utilised as a marker for placental dysfunction. PMID: 26955777
  28. Data suggest that plasma kisspeptin and serum prolactin levels may be involved in the physiopathology of breast enlargement in newborns. PMID: 26831552
  29. KISS1 Low Expression Correlates with Colorectal Liver Metastasis. PMID: 26471489
  30. KiSS1 is a metastasis suppressor gene of pancreatic cancer, and this suppression is not dependent on the expression levels of GPR54. PMID: 26572251
  31. confirmed that mutations in KISS1 and KISS1R are not a common cause for ICPP PMID: 23950571
  32. KiSS-1 downregulation may play a role in tumor progression and metastasis of oral squamous cell carcinoma, and may be a reliable biomarker for predicting clinical outcome in this disease. PMID: 26809635
  33. These findings are of tremendous significance and will greatly shape how KISS1 will be developed as a therapeutic agent in augmenting the reproductive potential of both women and important livestock species--{REVIEW} PMID: 26183891
  34. On D4 of pregnancy, the Kiss 1 null uterus expresses functional KISS1R molecules. PMID: 26384646
  35. Metastin levels were higher in women with polycystic ovary syndrome as compared to controls regardless of BMI. PMID: 25020276
  36. Metastin expression is induced in ESCs through decidualization. PMID: 24908069
  37. Present data do not confirm the protective role of KiSS1/KiSS1R in breast cancer progression, but our results do support the hypothesis that KiSS1/KiSS1R system is activated even in primary breast cancer and sustained during invasion to local lymph nodes. PMID: 25535062
  38. rs5780218 polymorphism individually confers susceptibility for development of breast cancer in Mexican population. PMID: 25810563
  39. The results suggest that mutations in the coding sequence of KISS1 are not common in patients with IHH in this Chinese population. PMID: 25783047
  40. A number of mutations in KISS1 KISS1R are associated with central precocious puberty and isolated hypogonadotropic hypogonadism. (Review) PMID: 26510589
  41. Colocalization experiments provided evidence for the presence of cocaine- and amphetamine-regulated transcript (CART) in KP-immunoreactive (IR) and perikarya and in KP-IR and NKB-IR axon varicosities. PMID: 25084101
  42. The genetic variations of KISS1 gene can be contributing factors of development of CPP. PMID: 25120323
  43. Serum kisspeptin levels were significantly lower in all infertile males as compared to the fertile males. PMID: 25556380
  44. KISS1 expression in the primary site of colorectal cancer could become a useful marker, with its increased levels indicating an advanced disease and a worse prognosis. PMID: 26010933
  45. Kiss-1 may be a putative metastasis suppressor molecule in human colorectal cancer. PMID: 25260785
  46. These data demonstrated that upregulated UHRF1 increases bladder cancer cell invasion by epigenetic silencing of KiSS1. PMID: 25272010
  47. Suggest that KISS1 is down-regulated in cancer tissues via promoter hypermethylation. PMID: 25110434
  48. KiSS-1, might be an important biological marker involved in the carcinogenesis, metastasis, and invasion of gallbladder adenocarcinoma. PMID: 25688501
  49. Kisspeptin effect on endothelial monocyte activating polypeptide II (EMAP-II)-associated lymphocyte cell death and metastases in colorectal cancer patients PMID: 24395571
  50. Decreased expression of KISS1R seems to attenuate signaling of the KISS1/KISS1R system, possibly leading to tumor growth PMID: 25667462
  51. Targeting the KiSS-1/GPR54 system may represent a novel therapeutic approach for cancers. PMID: 24377507
  52. SP may influence the KP and NKB secretory output via additional autocrine/paracrine mechanisms or regulate GnRH neurosecretion directly. PMID: 23977290
  53. Present results indicate that 18- and 24-h fasting conditions suppressed the testosterone response to KP10 administration both in initiation and quantity. PMID: 24500987
  54. Our findings indicate that the decreased expression of KiSS1 is correlated with distant metastasis of osteosarcoma, and KiSS1 may function as a tumor suppressor in OS cells through inhibition of the MAPK pathway. PMID: 24441183
  55. Stromal-derived factor 1-induced increase of endometrial tumor cell invasion is significantly reduced after treatment with Kiss-1. PMID: 24407584
  56. Kisspeptin, in a dose- and time-dependent manner, induces increased adhesion of human EVTs to type-I collagen, a major component of the human placenta. PMID: 24273038
  57. furin appears to be the essential enzyme for the generation of kisspeptins. PMID: 24454770
  58. decreased kisspeptin and PIBF expressions in trophoblasts and deciduas are associated with recurrent spontaneous abortion PMID: 24225150
  59. Sustained calcium response to kisspeptin is dependent on the continued presence of extracellular ligand and is the result of dynamic KISS1R trafficking. PMID: 24295737
  60. We report that circulating kisspeptin is elevated in both boys and girls when compared with adults. PMID: 23869023
  61. Loss of the metastasis suppressor gene KiSS1 is associated with lymph node metastasis in colorectal cancer. PMID: 23784200
  62. High KISS1 expression is associated with breast cancer. PMID: 24596387
  63. Metastasis suppressor KISS1 seems to reverse the Warburg effect by enhancing mitochondrial biogenesis. PMID: 24351292
  64. these studies demonstrate the antitumor activity of KISS1 in the breast cancer cell lines and provide insight into relevant mechanisms that may lead to novel treatments for breast cancer. PMID: 23900657
  65. The Kiss-1 gene has been suggested as a suppressor of metastasis in a various types of cancer, like gastric cancer, oesophageal carcinoma, pancreatic, ovarian, bladder and prostate cancer, by regulation of cellular migration and invasion. [Review] PMID: 23969598
  66. cumulative survival rate of patients with KISS1 or KISS1R expression was significantly higher compared to that without expression. KISS1 or KISS1R expression in non-small cell lung cancer can be used to indicate favorable prognosis for disease outcome. PMID: 23716269
  67. ZEB1/2 upregulation by WASF3 results from downregulation of KISS1, leading to the release of inhibition of nuclear factor (NF)kappaB by IkappaBalpha PMID: 23318438
  68. The evidence for epigenetic factors affecting Kiss1 expression during puberty and development, and findings regarding the contribution of epigenetics to the sexually dimorphic expression of Kiss1 in the hypothalamus. PMID: 23510953
  69. These findings suggest that kisspeptin, leptin, and RBP4 are associated with metabolic disturbances in women with polycystic ovaries. PMID: 23571154
  70. Data show that that the expression of Kiss1 Kiss1R and MMP9 were higher in the placenta compared to the placental bed and decidua parietalis. PMID: 23696833
  71. KISS1 expression is reduced during the malignant transformation of the colonic mucosa and there is a difference in the expression pattern between the right and the left part of the large intestine. PMID: 24033850
  72. In this review, alterations in the Kiss1 system appear to have a connection with the pathophysiology of polycystic ovary syndrome. PMID: 23809625
  73. [review] Exposure to sex steroids during critical periods of development can induce organizational (permanent) effects on kisspeptin neuron development, with respect to both sexually and non-sexually dimorphic aspects of kisspeptin biology. PMID: 22728025
  74. Brief exposure to kisspeptin results in sustained GnRH release lasting ~17 min in men. (2) Women in different phases of the menstrual cycle have differences in their response to kisspeptin PMID: 23550003
  75. Kisspeptin excitation of GnRH neurons. PMID: 23550004
  76. The development of peripherally active kisspeptin antagonists could offer a unique therapeutic agent for treating hormone-dependent disorders of reproduction, including precocious puberty, endometriosis, and metastatic prostate cancer. PMID: 23550006
  77. kisspeptin-10 transiently excites oxytocin neurons in late pregnancy and during lactation, suggesting that a central kisspeptin excitation of oxytocin neurons emerges at the end of pregnancy PMID: 23550008
  78. ontogeny, maturation, and sexual differentiation of kisspeptin neurons, as well as their regulation by sex steroids and other factors during development. PMID: 23550009
  79. In our model, ARC kisspeptin neurons play no "regulatory" role in controlling the timing of puberty. Rather, as a component of the neural network responsible for GnRH pulse generation PMID: 23550010
  80. Sex steroid regulation of kisspeptin circuits. PMID: 23550011
  81. Kisspeptin and GnRH pulse generation. PMID: 23550012
  82. Interactions between kisspeptins and neurokinin B. PMID: 23550013
  83. an integral and balanced view of the physiological relevance and potential mechanisms for the metabolic control of the kisspeptin system, as important pathway for the integral regulation of energy balance, puberty onset, and fertility. PMID: 23550015
  84. the significance of kisspeptin as a key locus of integration for circadian and steroidal signaling necessary for the initiation of ovulation. PMID: 23550016
  85. a novel role of kisspeptin signaling in the modulation of reproductive function by stress PMID: 23550018
  86. disruption of kisspeptin signaling pathways could have wide ranging effects across multiple organ systems PMID: 23550019
  87. kisspeptin and its receptor are critical for reproduction PMID: 23549999
  88. Structure, synthesis, and phylogeny of kisspeptin and its receptor. PMID: 23550000
  89. There are two major groups of kisspeptin cell bodies: a large number in the arcuate nucleus and a smaller collection in the rostral periventricular area of the third ventricle of rodents and preoptic area of non-rodents. PMID: 23550001
  90. KiSS-1 expression is also increased in placenta of intrauterine death. PMID: 23145030
  91. The antiproliferative effect of kisspeptin was only detectable in cells with artificial receptor expression. Based on this, the antiproliferative action of kisspeptin seems to be not relevant in the pathophysiological context. PMID: 23152107
  92. Studied plasma KP-54 levels in gastric cancer (GC) patients and healthy individuals and found KP-54 levels were significantly higher in GC patients (63.3+/-17.9) than in controls (49.0+/-12.7) (p=0.000). PMID: 22959969
  93. shown for the first time that human female adipose tissue may be a source of kisspeptin-1 PMID: 22899305
  94. Plasma metastin levels may be useful in the assessment of the severity of pre-eclampsia. PMID: 22769053
  95. study revealed that KiSS-1 is differentially expressed in colorectal cancer; it provided a mechanistic explanation for the identified loss of KiSS-1 cytoplasmic expression in colorectal malignancies by epigenetic silencing PMID: 23132294
  96. Data suggest that serum metastin is decreased during first trimester in women who later develop pre-eclampsia as compared to control subjects; serum placenta growth factor is decreased; serum chitotriosidase is increased. PMID: 22770630
  97. Negative fetal FSH/LH regulation in late pregnancy is associated with declined kisspeptin/KISS1R expression in the tuberal hypothalamus. PMID: 23015653
  98. The findings led to the hypothesis that kisspeptin may represent a placental proapoptotic agent acting in physiological and/or pathological pregnancy conditions in which placental apoptosis mechanisms are increased. PMID: 22556014
  99. these immunohistochemical data suggest an aging-related robust enhancement in central kisspeptin signaling PMID: 23011920
  100. The primate hypothalamic-pituitary-gonadal axis response to kisspeptin stimulation remains intact that to excitatory amino acids is attenuated in hypoglycemic conditions. PMID: 22473757

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Involvement in disease Hypogonadotropic hypogonadism 13 with or without anosmia (HH13)
Subcellular Location Secreted
Protein Families KISS1 family
Tissue Specificity Very high expression in placenta, with the next highest level in testis and moderate levels in pancreas, liver, small intestine and brain at much lower levels. Expression levels increased in both early placentas and molar pregnancies and are reduced in ch
Database Links

HGNC: 6341

OMIM: 603286

KEGG: hsa:3814

STRING: 9606.ENSP00000356162

UniGene: Hs.95008

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