Code | CSB-EL012373HU |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human KISS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 91 | ||||
Range % | 86-94 | |||||
1:2 | Average % | 101 | ||||
Range % | 97-103 | |||||
1:4 | Average % | 90 | ||||
Range % | 85-96 | |||||
1:8 | Average % | 95 | ||||
Range % | 91-100 |
The recovery of human KISS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 86 | 80-93 | ||||
EDTA plasma (n=4) | 96 | 92-100 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human KISS1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human KISS1 in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of KISS1 bound in the initial step.
KISS1 is quickly processed in serum into smaller but biologically active kisspeptins (KPs), which mainly regulate reproductive functions through its receptor KISS1R in mammals. KISS1/KPs/KISS1R signaling is involved in the onset of puberty and control of the hypothalamic-pituitary-gonadal axis. KPs play a role in numerous biological activities, including oocyte maturation, ovulation, spermatogenesis, testicular steroidogenesis, pregnancy, as well as placental migration and invasion. In many human tumors, KISS1 acts as a metastasis-inhibitor gene and KISS1/KISS1R signaling has anti-metastatic and tumor-suppressive roles in various cancers, including ovary, bladder, and colorectal cancers.
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Which molecule (peptides: kp-54, kp-10, kp-13, kp14) is detected in this kit?