Human Metastasis-suppressor KiSS-1(KISS1) ELISA kit

Code CSB-EL012373HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q15726
Lead Time 7-14 working days
Abbreviation KISS1
Target Name KiSS-1 metastasis-suppressor
Alias KiSS-1, METASTIN, MGC39258, kisspeptin|malignant melanoma metastasis-suppressor
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may function to inhibit chemotaxis and invasion, attenuating metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues.
HGNC 6341
RGD 727850
MGI 2663985
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human KISS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-94  
1:2 Average % 101  
Range % 97-103  
1:4 Average % 90  
Range % 85-96  
1:8 Average % 95  
Range % 91-100  
Recovery
The recovery of human KISS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 80-93  
EDTA plasma (n=4) 96 92-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.842 2.963 2.903 2.775  
10 2.187 2.203 2.195 2.067  
5 1.148 1.226 1.187 1.059  
2.5 0.755 0.743 0.749 0.621  
1.25 0.349 0.365 0.357 0.229  
0.625 0.222 0.205 0.214 0.086  
0.312 0.202 0.200 0.201 0.073  
0 0.132 0.124 0.128    
References
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Function Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
Involvement in disease Hypogonadotropic hypogonadism 13 with or without anosmia (HH13)
Subcellular Location Secreted
Protein Families KISS1 family
Tissue Specificity Very high expression in placenta, with the next highest level in testis and moderate levels in pancreas, liver, small intestine and brain at much lower levels. Expression levels increased in both early placentas and molar pregnancies and are reduced in ch
Database Links

HGNC: 6341

OMIM: 603286

KEGG: hsa:3814

STRING: 9606.ENSP00000356162

UniGene: Hs.95008

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