Name: Human Neurogranin(NRGN) ELISA kit
Brand: CUSABIO
SKU: CSB-EL016081HU

Product Details

Alternative Names

Protein kinase C substrate RC3 ELISA Kit; Calmodulin binding protein ELISA Kit; Hng ELISA Kit; NEUG(55-78) ELISA Kit; NEUG_HUMAN ELISA Kit; Neurogranin (protein kinase C substrate) ELISA Kit; Ng ELISA Kit; NRGN ELISA Kit; Protein kinase C substrate ELISA Kit; RC3 ELISA Kit

Abbreviation

NRGN

Uniprot No.

Q92686

Species

Homo sapiens (Human)

Sample Types

Detection Range

Sensitivity

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Materials provided

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human NRGN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled NRGN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

CUSABIO’s Neurogranin(NRGN) ELISA kit is specifically designed and validated for the quantitative detection of NRGN in the serum, plasma, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for neuroscience research use in humans. The kit has been validated with excellent sensitivity, high specificity, precision less than 10%, good linearity, high recovery, and tiny inter-batch differences. Refer to the product instructions for more details.

This assay employs the quantitative sandwich enzyme immunoassay technique, in which NRGN in the samples or standards are sandwiched between pre-coated NRGN antibody and Biotin-conjugated NRGN antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of NRGN bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of NRGN in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

NRGN is a small neuronal protein present mainly in the cell body and dendrites. It primarily seems to regulate calmodulin (CaM) signal transduction pathways of CaM-dependent enzymes to enhance synaptic plasticity in long-term potentiation (LTP). Keeping NRGN concentrations in a normal range in neurons is essential for proper cognition, while a reduction of NRGN upon aging and degenerative diseases may lead to negative cognitive symptoms. Increased levels of NRGN has been detected in the cerebrospinal fluid (CSF) of patients with Alzheimer's disease (AD).

Target Background

Function
(From Uniprot)

Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.

Gene References into Functions

The dynamics of calmodulin interactions with neurogranin and Ca(2+) /CAMKII alpha proteins has been reported. PMID: 28449373
Plasma neurogranin did not correlate with stroke severity. PMID: 28854881
Study observed an association of rs12807809 with schizophrenia in a South Indian population. This study contributes toward the establishment of neurogranin as a susceptibility gene for schizophrenia South Indian Population. PMID: 28389239
associations of neuromodulin and neurogranin to Alzheimer's disease PMID: 27604409
The results of this results showed that increased CSF neurogranin levels in Alzheimer Disease. PMID: 27018940
Polymorphisms in NRGN are associated with schizophrenia, major depressive disorder and bipolar disorder in the Han Chinese population. PMID: 26828755
Neurogranin and YKL-40 are promising AD biomarkers, independent of and complementary to the established core Alzheimer's disease (AD) biomarkers, reflecting additional pathological changes in the course of AD PMID: 26698298
These results confirm an increase in CSF Ng concentration in patients with AD as previously reported and show that this is specific to AD and not seen in a range of other neurodegenerative diseases. PMID: 26826204
This report provides evidence to support larger and controlled traumatic brain injury clinical studies for NRGN validation and prediction of outcomes. PMID: 26025774
Within-person levels of NGRN increased in cognitively normal participants but not in patients with later stage mild cognitive impairment or Alzhiemer's disease; NGRN may reflect presymptomatic synaptic dysfunction or loss. PMID: 26366630
Cerebrospinal fluid neurogranin was increased in patients with Alzheimer's dementia, progressive mild cognitive impairment (MCI) and stable MCI compared with controls, and in Alzheimer's dementia and progressive MCI compared with stable MCI. PMID: 26373605
neurogranin binds to alpha-synuclein in the human cortex, and this interaction decreases in Parkinson's disease along with the phosphorylation of neurogranin, a molecular process thought to be involved in learning and memory PMID: 25446004
These findings help to clarify underlying NRGN mediated pathophysiological mechanisms involving cortical-subcortical brain networks in schizophrenia. PMID: 24386483
Data indicate that neurogranin makes contacts with both the N- and C-domains of calmodulin that functionally leads to altered calcium binding kinetics. PMID: 24713697
NRGN risk variants contribute to subtle changes in neural functioning and anatomy. PMID: 24098564
Our results support an association between the NRGN gene and schizophrenia and a hypothesis that the NRGN gene may mediate the risk associated with schizophrenia via intellectual dysfunction. PMID: 23903071
The influence of NRGN genotype on the neural correlates of memory encoding and retrieval is manifest in the cingulate cortex and is involved in hippocampal formation. PMID: 21799211
single nucleotide polymorphism located upstream of the neurogranin (NRGN) gene has been identified as a risk variant for schizophrenia. PMID: 22856365
This study demonistrated that multiple rare mutations in schizophrenia, and provides genetic clues that indicate the involvement of NRGN in this disorder. PMID: 22306195
This study provides further evidence of the association of the NRGN gene with schizophrenia. PMID: 22461181
The genome-wide associated genetic risk variant in the NRGN gene may be related to a small gray matter volume in the anterior cingulate cortex in the left hemisphere in patients with schizophrenia. PMID: 22253779
Hippocampal activation diminished during the acquisition of contextual fear in healthy carriers of the genome-wide-supported risk variant for schizophrenia, rs12807809 in neurogranin. PMID: 21647148
our study provides strong evidence that common exonic variation does not account for the genome-wide signi fi cant association between schizophrenia and variation at NRGN PMID: 21538840
Elevated cerebrospinal fluid neurogranin levels together with Alzheimer disease biomarkers may reflect synaptic degeneration. PMID: 20875798
No association between the schizophrenia associated NRGN variant rs12807809 and cognition could be detected in these samples. PMID: 21112188
IL-2 deprivation raises the level of RC3 and other apoptotic factors, which induce apoptosis by increasing the intracellular Ca(2+) concentration PMID: 12808095
Nevertheless, by gel shift assays, Sp1 and Sp3 were not found to be responsible for the protein-DNA complexes formed by the GC-rich sequence. PMID: 16677608
Genotype distribution showed association of the rs7113041 SNP with schizophrenia in males of Portuguese origin, which was confirmed by the analysis of the proband-parent triads. PMID: 17140601
Data suggest that BSX is essential for global cognitive function and that haplo-insufficiency may cause severe mental retardation, and that deletion of Neurogranin contributes to the auditory attention deficit observed in most 11q- patients. PMID: 18855024

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Protein Families

Neurogranin family

Tissue Specificity

In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease.

Database Links

HGNC: 8000

OMIM: 602350

KEGG: hsa:4900

STRING: 9606.ENSP00000284292

UniGene: Hs.524116

Code	CSB-EL016081HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Human Neurogranin(NRGN) ELISA kit

Product Details

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Target Background