Human Neurturin(NRTN) ELISA kit

Code CSB-EL016095HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
neurturin
Alternative Names
Neurturin ELISA Kit; Neurturin precursor ELISA Kit; NRTN ELISA Kit; NRTN_HUMAN ELISA Kit; NTN ELISA Kit; prepro-neurturin ELISA Kit
Abbreviation
NRTN
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
125 pg/mL-8000 pg/mL
Sensitivity
31.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NRTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-97  
1:2 Average % 103  
Range % 97-108  
1:4 Average % 97  
Range % 92-102  
1:8 Average % 94  
Range % 89-99  
Recovery
The recovery of human NRTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 86-97  
EDTA plasma (n=4) 104 98-109  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
8000 2.546 2.664 2.605 2.470  
4000 2.196 2.075 2.136 2.001  
2000 1.464 1.489 1.477 1.342  
1000 0.836 0.822 0.829 0.694  
500 0.428 0.433 0.431 0.296  
250 0.283 0.294 0.289 0.154  
125 0.196 0.190 0.193 0.058  
0 0.136 0.134 0.135    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

Neurturin, a protein encoded by the NRTN gene in human, plays a critical role in the development and maintenance of dopaminergic neurons and motor neurons. This Human Neurturin ELISA kit provides a reliable and sensitive method for the detection of human Neurturin in a range of sample types, including serum, plasma, and tissue homogenates.

With a detection range of 125 pg/mL to 8000 pg/mL and a sensitivity of 31.25 pg/mL, this  ELISA kit enables precise and accurate measurement of Neurturin levels in your samples. The assay time is only 1-5 hours, and the sample volume required is only 50-100ul, making it a fast and efficient tool for your research.

The Human Neurturin ELISA kit follows a sandwich assay principle, providing robust and reproducible results. The detection wavelength is 450 nm, ensuring high sensitivity and specificity. This kit is an excellent choice for researchers studying the molecular mechanisms of dopaminergic neurons and other areas of neuroscience.

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Target Background

Function
(From Uniprot)
Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.
Gene References into Functions
  1. biophysical results show that the relative concentration of GFRa2 on cell surfaces can affect the functional affinity of NRTN through avidity effects. PMID: 29414779
  2. In the cochlea, NTN immunostaining was found in the supporting cells of organ of Corti, including Deiters' cells, Hensen cells as well as Claudius' cells. In the spiral ganglia, NTN was seen in both the cell bodies and the nerve fibers of neurons. PMID: 24139947
  3. Neurturin contributes toward an aggressive cancer cell phenotype, neuropathic pain and neuronal plasticity in pancreatic cancer. PMID: 24067900
  4. Human neurturin protected crayfish neurons and glia from photodynamic injury. PMID: 22847529
  5. Cyclic AMP signalling through PKA but not Epac is essential for neurturin-induced biphasic ERK1/2 activation and neurite outgrowths through GFRalpha2 isoforms. PMID: 21723942
  6. GDNF and NRTN are new neuromodulators that regulate the development of the neuromuscular synapse PMID: 11790765
  7. The gene expression of protein was studied in the developing human tooth. PMID: 12397373
  8. The results obtained suggest the involvement of NTN, PSP, and ART in processes subserving both the organization of this cortical region during development and the functional activity and maintenance of the mature human hippocampal neurons. PMID: 15829225
  9. Addition of neurturin to activated peripheral blood mononuclear leukocytes reduces the amount of detectable tumor necrosis factor protein without altering its transcription. PMID: 16081799
  10. GDNF and NTN differentially regulate the expression of distinct miRNA precursors through the activation of mitogen-activated protein kinase PMID: 16895582
  11. Depending on the brain's expressions of specific GDNF family GFRalpha2 receptor spliced isoforms, neurturin may promote or inhibit neurite outgrowth through the multicomponent receptor complex. PMID: 17522305
  12. GDNF, NTN, GFRalpha-1, GFRalpha-2, and c-Ret proteins are differentially expressed in the different stages of hair follicle cycle. GFRalpha-mediated signaling involves c-Ret and may play a role in human HF biology. PMID: 18222320
  13. No differences were found in the allelic frequencies of the variants or in the haplotype distribution between Hirschsprung's disease patients & controls, nor to any demographic/clinical parameters within the group of patients. PMID: 18970938

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Involvement in disease
Genetic variations in NRTN may contribute to Hirschsprung disease, in association with mutations of RET gene, and possibly mutations in other loci. Hirschsprung disease is a disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Subcellular Location
Secreted.
Protein Families
TGF-beta family, GDNF subfamily
Database Links

HGNC: 8007

OMIM: 602018

KEGG: hsa:4902

STRING: 9606.ENSP00000302648

UniGene: Hs.234775

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