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Human Nidogen 1 (NID1) ELISA Kit

Human Nidogen 1 (NID1) ELISA Kit

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Protocol

Code	CSB-E13289h
Size	96T,5×96T,10×96T
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Target Background

Product Details

Alternative Names

Enactin ELISA Kit; Ent ELISA Kit; Entactin 1 ELISA Kit; Entactin ELISA Kit; NID 1 ELISA Kit; NID ELISA Kit; NID-1 ELISA Kit; Nid1 ELISA Kit; NID1_HUMAN ELISA Kit; Nidogen (enactin) ELISA Kit; Nidogen 1 ELISA Kit; Nidogen ELISA Kit; Nidogen-1 ELISA Kit

Abbreviation

NID1

Uniprot No.

P14543

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

0.39 ng/mL-25 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	3.046	3.371	2.917	3.287	3.039	3.399
SD	0.038	0.035	0.04	0.055	0.049	0.057
CV(%)	4.455	3.885	4.802	6.187	5.751	6.298

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NID 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

82-93

1:2

Average %

Range %

94-100

1:4

Average %

Range %

90-104

1:8

Average %

Range %

82-93

Recovery

The recovery of human NID 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	101	96-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











ng/ml	OD1	OD2	Average	Corrected
25	2.186	2.140	2.163	2.016
12.5	1.642	1.633	1.638	1.491
6.25	1.251	1.220	1.236	1.089
3.12	0.873	0.830	0.852	0.705
1.56	0.579	0.541	0.560	0.413
0.78	0.332	0.342	0.337	0.190
0.39	0.260	0.265	0.263	0.116
0	0.148	0.145	0.147

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human NID1 ELISA Kit was designed for the quantitative measurement of Human NID1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.39 ng/mL-25 ng/mL and the sensitivity is 0.09 ng/mL.

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Target Background

Function
(From Uniprot)

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Gene References into Functions

Results demonstrated that NID1 was a mesenchymal associated gene and its high expression was significantly correlated with shorter overall survival of ovarian cancer patients. The study presents evidence that NID1 promotes the migration, invasion and chemoresistance of ovarian cancer cells via EMT and its molecular mechanism involves the activation of ERK/MAPK signaling. PMID: 28416770
Nidogen 1 (NID1) was confirmed to promote lung metastasis of breast cancer and melanoma. PMID: 28827399
nidogen-1 degraded by CatS can be quantified in serum by the NIC assay. The current data strongly suggest that cathepsin-S degradation of nidogen-1 is strongly associated with NSCLC, which needs validation in larger clinical cohorts. PMID: 28282545
Differential protein expression of collagen IV, laminin alpha2, and nidogen-1 indicated basal lamina remodeling develops in ischemic failing versus nonfailing human hearts. PMID: 26756417
NID1 was confirmed as a target gene of MiR-192/215 in Hirschsprung's disease patients. PMID: 25857602
Increase of NID1 protein expression is associated with endometrial cancer invasion. PMID: 25924802
The cleavage of nidogen-1 and -2 is partially inhibited in human tumor samples. PMID: 23681936
results demonstrate that mutation in either NID1 or LAMC1 disrupts the interaction NID1-LAMC1 complex; findings implicate the extracellular matrix in the pathogenesis of Dandy-Walker spectrum disorders PMID: 23674478
Cleavage of nidogen-1 by cathepsin S impairs its binding to basement membrane partners PMID: 22952693
NID1 gene is a biologically plausible locus for nevogenesis and melanoma development PMID: 21478494
found to be ubiquitous component of basement membrane zones underneath developing epithelia of most of the major organ systems; may participate in cell biological functions during human development PMID: 12005023
nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone PMID: 17008882
CpG islands of both NID1 and NID2 genes are aberrantly methylated in human cancer samples and cancer cell lines. PMID: 17328794

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Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Database Links

HGNC: 7821

OMIM: 131390

KEGG: hsa:4811

STRING: 9606.ENSP00000264187

UniGene: Hs.356624

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Human Nidogen 1 (NID1) ELISA Kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background