This Human Osteocalcin/Bone gla protein (BGLAP) ELISA kit allows for the in vitro quantitative determination of BGLAP concentrations in serum, plasma, or tissue homogenates. BGLAP, also known as OCN (osteocalcin), is a non-collagenous, vitamin K-dependent protein expressed and secreted exclusively by osteoblasts. It contains three gamma-carboxyglutamic acid (Gla) motifs that facilitate mineral deposition and bone remodeling in the presence of calcium. BGLAP acts as a regulator of the activity of osteoclasts and their precursors. The uncarboxylated BGLAP can act on the pancreas, adipose tissue, male gonads, and muscle to stimulate insulin secretion, improve insulin sensitivity, male fertility, and muscle power through the receptor Gprc6a.
Based on the sandwich ELISA technique, BGLAP in the sample is sandwiched between pre-coated NGAL antibody and Biotin-conjugated NGAL antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and then develops a color reaction after the addition of the TMB substrate solution. There is a positive correlation between the solution color intensity and the BGLAP content in the sample. This kit has many advantages, including a broad detection range (15.6 pg/mL-1000 pg/mL), high sensitivity, excellent specificity, and precision less than 10%. Get access to more validation details in the product instructions.