Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit

Instructions
Code CSB-E05128h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human Osteocalcin/Bone gla protein (BGLAP) ELISA kit allows for the in vitro quantitative determination of BGLAP concentrations in serum, plasma, or tissue homogenates. BGLAP, also known as OCN (osteocalcin), is a non-collagenous, vitamin K-dependent protein expressed and secreted exclusively by osteoblasts. It contains three gamma-carboxyglutamic acid (Gla) motifs that facilitate mineral deposition and bone remodeling in the presence of calcium. BGLAP acts as a regulator of the activity of osteoclasts and their precursors. The uncarboxylated BGLAP can act on the pancreas, adipose tissue, male gonads, and muscle to stimulate insulin secretion, improve insulin sensitivity, male fertility, and muscle power through the receptor Gprc6a.

Based on the sandwich ELISA technique, BGLAP in the sample is sandwiched between pre-coated NGAL antibody and Biotin-conjugated NGAL antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and then develops a color reaction after the addition of the TMB substrate solution. There is a positive correlation between the solution color intensity and the BGLAP content in the sample. This kit has many advantages, including a broad detection range (15.6 pg/mL-1000 pg/mL), high sensitivity, excellent specificity, and precision less than 10%. Get access to more validation details in the product instructions.

Target Name bone gamma-carboxyglutamate (gla) protein
Alternative Names BGLAP ELISA Kit; BGP ELISA Kit; Bone gamma carboxyglutamate (gla) protein ELISA Kit; Bone gamma carboxyglutamate gla protein osteocalcin ELISA Kit; Bone gamma carboxyglutamate protein ELISA Kit; Bone Gla protein ELISA Kit; Gamma carboxyglutamic acid containing protein ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit; OC ELISA Kit; OCN ELISA Kit; OSTCN_HUMAN ELISA Kit; Osteocalcin ELISA Kit; OTTHUMP00000016586 ELISA Kit; PMF1 ELISA Kit
Abbreviation BGLAP
Uniprot No. P02818
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/ml-2000 pg/ml
Sensitivity 7.8 pg/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision  
Linearity  
Recovery  
Typical Data  
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human BGLAP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled BGLAP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Gene References into Functions
  1. The results of this study suggest that serum osteocalcin is associated with the onset of puberty in girls. PMID: 30089739
  2. The correlation between Mycobacterium avium subsp. paratuberculosis (MAP) infection and elevation of sera ucOC, reduction of active osteocalcin and increased calcium supports MAP infection role in Crohn's disease and complications with osteoporosis. PMID: 29638140
  3. The results showed no significant correlation between osteocalcin HindIII gene polymorphism and bone mineral density (BMD) and therefore osteocalcin HindIII gene polymorphism may not have major effects on BMD variation in postmenopausal North Indian women. PMID: 30179426
  4. Decreased circulating carboxylated osteocalcin is associated with low-energy bone fractures in children. PMID: 29882816
  5. a decrease in serum osteocalcin levels is associated with future development of type 2 diabetes mellitus. PMID: 28766135
  6. Findings from the available data indicated an overall significant inverse association between serum osteocalcin and body mass index--{REVIEW} PMID: 28822067
  7. relatively lower serum osteocalcin levels were associated with a higher risk of MACE in Chinese men with CAD PMID: 28921598
  8. undercarboxylated form of osteocalcin in serum not related significantly to semen parameters or measured hormones in male infertility PMID: 28000970
  9. serum level not correlated with gender, race/ethnicity, age, diabetes type, BMI percentile, random C-peptide, glucose, or HbA1c at pediatric diabetes diagnosis; serum level inversely correlated with HbA1c at first office visit following diagnosis PMID: 28093839
  10. Serum osteocalcin was directly and independently correlated with circulating uric acid in a population of healthy obese subjects. PMID: 28847266
  11. gamma-carboxylation of OCN on Glu17 residue correlates with insulin resistance and subclinical inflammation, suggesting that gamma-carboxylation of OCN negatively regulates its endocrine action PMID: 28242268
  12. There were differences in the associations between total osteocalcin and glucose metabolism in Han and Uygur T2DM patients, indicating genetic factors may play a role in modulating OC and glucose metabolism in different ethnic population. PMID: 28732499
  13. The study demonstrated that undercarboxylated osteocalcin (ucOC) is related to 25(OH)D and adiponectin concentrations. Both ucOC and 25(OH)D were lower in overweight and obese adolescents. PMID: 28443718
  14. Increased advanced glycation end products and protein oxidative damage markers in the course of obesity may contribute to decreased carboxylated osteocalcin serum level. PMID: 28841723
  15. Progressive calcification of atherosclerotic plaques is accompanied by insignificant accumulation of calcitonin and osteoprotegerin, whereas the osteocalcin expression significantly increased during calcification. PMID: 28429221
  16. Undercarboxylated osteocalcin reflects the reserve capacity of beta-cell function, such as the bolus insulin secretion ability in patients with type 2 diabetes. PMID: 27889949
  17. XPS showed that similar amounts of hOC and dhOC were absorbed onto hydroxyapaptite and octacalcium phosphate surfaces, but ToF-SIMS detected some small differences in the amino acid fragment intensities on these surfaces for adsorbed hOC and dhOC. PMID: 28521505
  18. Common polymorphism rs1800247 in osteocalcin gene was associated with hypertension and diastolic blood pressure levels. The association of rs1800247 with hypertension was affected by its interaction with HOMA-IR. PMID: 27251081
  19. These findings indicate the undercarboxylated form of OC is not implicated in age-related changes in skeletal muscle or adipose tissue mass in older community-dwelling adults. PMID: 27604070
  20. Demonstrate a positive association between osteocalcin, a factor known to regulate bone remodelling, with cognitive performance in older non-demented women. PMID: 27515675
  21. Common genetic variants of the osteocalcin gene may not be a major contributor to variations in serum osteocalcin or BMD in postmenopausal and elderly Chinese women. PMID: 28002816
  22. Several detected relations between OCN and metabolites were strongly linked to renal function, however, a number of associations remained significant after adjustment for renal function. Intermediates of proline catabolism were associated with OCN reflecting the implication in bone metabolism. PMID: 28922389
  23. Low serum osteocalcin concentrations are associated with type 2 diabetes. PMID: 27061949
  24. Decreased blood concentration of Carboxylated osteocalcin is associated with metabolic complications and inflammation. PMID: 27667744
  25. Total and undercarboxylated osteocalcin levels were significantly correlated with insulin secretion and insulin resistance indices, but not adiponectin levels, in pregnant women. PMID: 26708046
  26. Osteocalcin and Sex Hormone Binding Globulin Compete on a Specific Binding Site of GPRC6A. PMID: 27673554
  27. Osteocalcin plays an essential role for the maturation of mineral species and modulates osteogenic differentiation of mesenchymal stromal cells (MSCs). PMID: 28106724
  28. Serum osteocalcin levels in pediatric obstructive sleep apnea hypopnea syndrome patients were significantly lower than normal controls before adenotonsillectomy. PMID: 26938749
  29. the relationship between serum osteocalcin and glucose metabolism parameters in liver transplantation recipients, was examined. PMID: 26743527
  30. Reduced plasma Osteocalcin levels are associated with cardiovascular and metabolic diseases. PMID: 26863345
  31. An overview of osteocalcin progress. PMID: 26747614
  32. the BMP-2/Smad1/5/RUNX2 signaling pathway participates in the silicon-mediated induction of COL-1 and osteocalcin synth PMID: 27025722
  33. Data indicate that lower osteocalcin was an independent predictor of worse cognitive performance on multivariate analysis. PMID: 26406918
  34. A common polymorphism rs1800247 in osteocalcin gene may effect the risk of osteoporosis and fracture and serum osteocalcin levels in Chinese. PMID: 26194493
  35. There is an inverse relationship between the visfatin and undercarboxylated osteocalcin in type 2 diabetes patients with diabetic kidney disease. PMID: 26922969
  36. This study investigated osteocalcin, metabolic parameters and anthropometric characteristics in normal weight and overweight/obese children. PMID: 26772621
  37. Nonalcoholic fatty liver disease negatively associated with right-hip bone mineral density and serum osteocalcin in Korean men. PMID: 26636404
  38. The aims of this study were to evaluate OCN and sclerostin levels in subjects who underwent coronary artery bypass graft (CABG) surgery compared with those in normal controls. PMID: 26576474
  39. serum level not associated with weight loss or body fat percentage PMID: 25963022
  40. Serum osteocalcin levels are inversely correlated with nonalcoholic fatty liver disease in postmenopausal Chinese women with normal blood glucose levels. PMID: 26567728
  41. Serum total osteocalcin level is significantly lower in 2 diabetes mellitus patients than controls. PMID: 26372899
  42. Elevated serum preptin and decreased osteocalcin concentrations, together with insulin resistance, are associated with obesity and overweight. PMID: 25675353
  43. gestational diabetes is not associated with osteocalcin, under-carboxylated osteocalin, osteopontin and leptin and does not correlate with insulin resistance. PMID: 25480426
  44. Serum uOC levels in non-dialysis patients with CKD are significantly lower than those in healthy individuals, and uOC is closely associated with subclinical atherosclerosis in CKD patients. PMID: 26381729
  45. Endocrine function of osteocalcin and the cell biology events that allow osteocalcin to become a hormone. [review] PMID: 25577163
  46. Data indicate that higher osteocalcin (OC) or its undercarboxylated form (ucOC) concentration and ucOC to osteocalcin ratio (OCR) were associated with the risk of coronary calcification in men. PMID: 25868823
  47. Data suggest that a low serum osteocalcin level was an independent risk factor for abdominal obesity. PMID: 25871789
  48. Correlation between osteocalcin-positive endothelial progenitor cells and spotty calcification in patients with coronary artery disease PMID: 25644945
  49. These findings unequivocally document that increased vitamin K intake reduces the uncarboxylated form of OCN PMID: 25855755
  50. Serum osteocalcin level was not independently associated with C-IMT in a metabolically healthy Chinese population. PMID: 26077201

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Subcellular Location Secreted
Protein Families Osteocalcin/matrix Gla protein family
Database Links

HGNC: 1043

OMIM: 112260

KEGG: hsa:632

STRING: 9606.ENSP00000357255

UniGene: Hs.654541

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