24T ELISA Kit Trial Size (Only USD$150/ kit)
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The human OPG ELISA Kit allows for the in vitro quantitative determination of OPG concentrations in serum, plasma, or tissue homogenates. This kit exclusively recognizes human OPG. OPG, also known as TNFRSF11B, is closely involved in bone remodeling through interaction with RANKL. OPG-RANKL interaction inhibits the maturation and activation of pre-osteoclasts by antagonizing RANK-RANKL signaling thereby suppressing osteoclast function and facilitating bone formation. Declined OPG expression or an imbalanced RANKL/OPG ratio leads to osteoporosis, periodontitis, and rheumatoid arthritis. In addition to its role in bone homeostasis, OPG is implicated in various physiological and pathological processes, such as immune regulation, vascular function, and fibrosis. OPG-TRAIL signaling prevents the induction of apoptosis. OPG expression has also been found in various cancer types and is associated with tumor survival, epithelial to mesenchymal transition (EMT), neo-angiogenesis, invasion, and metastasis.
The detection mechanism of this kit is based on the Sandwich-ELISA technique. OPG in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled OPG antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with OPG content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.
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