Human Peptidase inhibitor 16(PI16) ELISA kit

Code CSB-EL017951HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
peptidase inhibitor 16
Alternative Names
Cripi ELISA Kit; CRISP 9 ELISA Kit; CRISP-9 ELISA Kit; CRISP9 ELISA Kit; Cysteine rich protease inhibitor ELISA Kit; Cysteine rich secretory protein 9 ELISA Kit; Cysteine-rich secretory protein 9 ELISA Kit; Microseminoprotein beta binding protein ELISA Kit; MSMBBP ELISA Kit; Peptidase inhibitor 16 ELISA Kit; PI-16 ELISA Kit; PI16 ELISA Kit; PI16_HUMAN ELISA Kit; Protease inhibitor 16 ELISA Kit; PSP94 binding protein ELISA Kit; PSP94-binding protein ELISA Kit; PSPBP ELISA Kit
Abbreviation
PI16
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-10 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<20%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PI16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 83-98
1:2 Average % 95
Range % 88-105
1:4 Average % 90
Range % 84-105
1:8 Average % 95
Range % 89-100
Recovery
The recovery of human PI16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 91-105
EDTA plasma (n=4) 95 86-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average  
10 2.254 2.266 2.260  
5 1.342 1.354 1.348  
2.5 0.662 0.669 0.666  
0.625 0.197 0.211 0.204  
0.312 0.099 0.102 0.101  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PI16 ELISA Kit was designed for the quantitative measurement of Human PI16 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-10 ng/mL and the sensitivity is 0.156 ng/mL.

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Target Background

Function
(From Uniprot)
May inhibit cardiomyocyte growth.
Gene References into Functions
  1. at high endothelial shear stress, PI16 contributes to inhibition of MMP2 protease activity, protection that can be reversed during inflammation PMID: 27996045
  2. PI16-positive Treg showed enhanced in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation PMID: 22533972
  3. A new blood protein PSP94-binding protein (PSPBP) is identified that binds prostate secretory protein of 94 amino acids (PSP94). PMID: 15344909
  4. a novel and independent prognostic markers following radical prostatectomy for prostate cancer. PMID: 17062675
Subcellular Location
Secreted.
Protein Families
CRISP family
Tissue Specificity
Expressed in prostate, testis, ovary and intestine. Concentrates in prostate cancer patient's sera.
Database Links

HGNC: 21245

KEGG: hsa:221476

STRING: 9606.ENSP00000362778

UniGene: Hs.25391

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