Human Protamine 1,PRM1 ELISA Kit

Code CSB-E09630h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
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Product Details

Target Name
protamine 1
Alternative Names
PRM1Sperm protamine P1 ELISA Kit; Cysteine-rich protamine ELISA Kit
Abbreviation
PRM1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PRM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 80-98  
1:2 Average % 97  
Range % 91-105  
1:4 Average % 98  
Range % 92-110  
1:8 Average % 93  
Range % 86-102  
Recovery
The recovery of human PRM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 85-104  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.786 2.752 2.769 2.613  
1000 2.306 2.376 2.341 2.185  
500 1.648 1.654 1.651 1.495  
250 0.985 1.010 0.998 0.842  
125 0.677 0.664 0.671 0.515  
62.5 0.503 0.509 0.506 0.350  
31.25 0.275 0.284 0.280 0.124  
0 0.154 0.157 0.156    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PRM1 ELISA Kit was designed for the quantitative measurement of Human PRM1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

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Target Background

Function
(From Uniprot)
Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex.
Gene References into Functions
  1. The expression level of PRM1 was significantly higher in colon cancer tissues and the staining degree of PRM1 in poorly-differentiated was stronger. PMID: 29140788
  2. Bacterial infections have significant negative effects on sperm chromatin condensation and protamine P1/P2 ratio. PMID: 28736810
  3. Protamine-1 and protamine-2 mRNA levels as well as the protamine mRNA ratio and all routine semen parameters revealed significant differences between recurrent miscarriage couples and healthy volunteers. Values of protamine-1 and protamine-2 mRNAs were significantly higher and the protamine mRNA ratio was significantly lower in couples with recurrent miscarriage. PMID: 28854581
  4. examinded the association between protamine gene alleles (PRM1 c.-190C>A, PRM1 c.197G>T, and PRM2 c.248C>T), and YBX2 (c.187T>C and c.1095 + 16A>G) and male infertility. We found that the AA and CA genotypes of the PRM1 c.-190C>A polymorphism had a significant association with infertility (p < 0.001) and the AA genotype was also highly significantly associated with high sperm DNA damage (p < 0.001). PMID: 29227750
  5. The KDM3A to PRM1 mRNA expression ratio can be used as a reliable marker of successful testicular sperm extraction in men with obstructive and non-obstructive azoospermia with 95% sensitivity. PMID: 27027467
  6. Single nucleotide polymorphisms in the human PRM1 gene are associated with a genetic risk factor for idiopathic oligozoospermia. PMID: 27216534
  7. Studies indicate that ribonucleotide reductase M1 polypeptide (RRM1) expression is associated with the response rate and overall survival rate of advanced NSCLC patients treated with gemcitabine-based chemotherapy. PMID: 26092210
  8. The expressed human protamine1 in two fast-growing cell systems, E. coli and HeLa cells. The protamine 1 expression significantly attenuated cell proliferation when compared with control cells. PMID: 25649960
  9. the quantity of PRM1, PRM2, and TNP2 transcripts and the PRM1/PRM2 mRNA ratio affect spermiogenesis, sperm morphology PMID: 25536093
  10. Single nuclotide polymorphisms in PRM1 gene is associated with male infertility. PMID: 22104739
  11. genetic association study in Han population in China: Data suggest that an SNP in PRM1 (rs35576928; T allele; 34R>S) is associated with severe oligozoospermia and can play a protective role against this disease. PMID: 23079002
  12. The SNP of PRM1-190C- > A might be associated with teratozoospermia-induced male infertility in the Han Chinese. PMID: 22574365
  13. significant correlation has been found between protamine 1 and 2 transcripts/proteins and: sperm concentration, progressive sperm motility, sperm fertilization ability as well as embryo quality PMID: 22472940
  14. identified and we report for the first time five novel rare haplotypes encompassing the protamine 1 and 2 genes PMID: 21029114
  15. Data suggest that Casp4, Casp 6 and TNFSF10 are differentially expressed in potentially fertile and subfertile men and represent useful biomarkers for predicting male fertility in combination with P1 and P2. PMID: 21317160
  16. Mutations in the protamine locus may be an infrequent cause of male infertility. PMID: 19602509
  17. single nucleotide polymorphisms in this gene in fertile and infertile males PMID: 12569175
  18. A number of SNPs are present in the testis-specific nuclear protein genes. PMID: 16989827
  19. The P1/P2 ratio has a significant relationship to sperm fertilization ability. PMID: 17011555
  20. Mutations in PRM1 gene were identified in males with oligozoospermia. PMID: 17494104
  21. The PRM1 contributes not only to successful fertilization, but may has an important impact in development of preimplantation embryos. PMID: 18292840
  22. A reduction in contents of PRM1 transcript in spermatozoa may be linked with asthenozoospermia. PMID: 18292846
  23. Decreased pre-P2/P2 and P1/P2 ratios are related to a poor pregnancy outcome, but not with the proportion of embryos obtained after assisted reproduction. PMID: 18314125
  24. Low-frequency protamine 1 gene transversions c.102G->T and c.-107G->C do not correlate with male infertility. PMID: 18381856
  25. Histones localized to the posterior ring region (i.e. the sperm nuclear annulus), whereas PRM1 and PRM2 appeared to be dispersed throughout the entire nucleus. PMID: 18478156
  26. The CCNA1, CCNB1, CCNB2, PRM1, and PRM2 messenger RNA transcript ratios were significantly decreased in patients with spermatogenic disorders. Transcript ratios in patients with successful sperm retrieval were higher than with failed sperm retrieval. PMID: 18692784

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Subcellular Location
Nucleus. Chromosome.
Protein Families
Protamine P1 family
Tissue Specificity
Testis.
Database Links

HGNC: 9447

OMIM: 182880

KEGG: hsa:5619

STRING: 9606.ENSP00000310515

UniGene: Hs.2909

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