Human Protein kinase C-binding protein NELL1(NELL1) ELISA kit

Instructions
Code CSB-EL015709HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name NEL-like 1 (chicken)
Alternative Names FLJ45906 ELISA Kit; IDH3GL ELISA Kit; NEL like 1 (chicken) ELISA Kit; NEL like protein 1 ELISA Kit; Nel related protein 1 ELISA Kit; NEL-like 1 (chicken) ELISA Kit; NEL-like protein 1 ELISA Kit; Nel-related protein 1 ELISA Kit; NELL 1 protein short isoform ELISA Kit; Nell1 ELISA Kit; NELL1_HUMAN ELISA Kit; Neural epidermal growth factor like 1 ELISA Kit; NRP1 ELISA Kit; Protein kinase C binding protein NELL1 ELISA Kit; Protein kinase C-binding protein NELL1 ELISA Kit
Abbreviation NELL1
Uniprot No. Q92832
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NELL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 91  
Range % 86-95  
1:10 Average % 102  
Range % 97-107  
1:20 Average % 91  
Range % 85-97  
1:40 Average % 97  
Range % 91-103  
Recovery
The recovery of human NELL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 92 88-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.907 2.987 2.947 2.768  
1000 2.260 2.208 2.234 2.055  
500 1.428 1.435 1.432 1.253  
250 0.841 0.865 0.853 0.674  
125 0.538 0.568 0.553 0.374  
62.5 0.398 0.377 0.388 0.209  
31.25 0.298 0.271 0.285 0.106  
0 0.183 0.175 0.179    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Plays a role in the control of cell growth and differentiation. Promotes osteoblast cell differentiation and terminal mineralization.
Gene References into Functions
  1. Results show that NELL1 methylation level was 6.8% higher in renal cell cancer (RCC) tissues associated with advanced disease of distant metastases, and shorter RFS. Analyses suggested that NELL1 DNA methylation is a promising candidate prognostic epigenetic biomarker for more detailed analyses of RCC disease. PMID: 30272321
  2. This work indicates that NELL-1, HMGB1, and CCN2 might enhance bone defect healing via the recruitment of endogenous cells and induction of vascularization and act via different processes than BMP2. PMID: 28463604
  3. NELL1 variant is associated with tobacco- and HPV-mediated oral oncogenesis. PMID: 30091681
  4. Results demonstrate that NELL1 is differentially expressed in fusion-positive alveolar rhabdomyosarcoma (ARMS) and in embryonal rhabdomyosarcoma (ERMS) samples, and they show that this transcriptional difference partially depends on genomic DNA methylation. The study also found that high NELL1 levels are correlated with negative RMS prognostic factors and with poor tumor outcomes. PMID: 28380437
  5. lipoaspirate-derived hPSCs present a novel and abundant cell source of MSCs for cartilage regeneration, and the combinatorial application of NELL-1, TGF-beta3, and BMP-6 with hPSCs may remarkably enhance and accelerate cartilage repair. PMID: 26700847
  6. establish the feasibility of combining NELL-1 with BMP2 to improve clinical bone regeneration and provide mechanistic insight into canonical Wnt pathway activity during NELL-1 and BMP2 osteogenesis PMID: 26772960
  7. Data suggest that TSPN (N-terminal thrombospondin-1-like) domain of NELL1 exhibits major heparin-binding sites which may be involved in interaction of NELL1 with cell surface heparan sulfate proteoglycans. PMID: 26627376
  8. NELL-1 signaling activates Wnt/beta-catenin signaling. PMID: 26082355
  9. We also detected lower relative expression of Nell-1 by real-time PCR. Furthermore, immunohistochemical analyses revealed that Nell-1 staining was less intense in cancer tissue relative to normal tissue and that the tumor cells had spread to the muscle PMID: 26090379
  10. CpG islands in the NELL1 and NELL2 promoters are hypermethylated in renal cell carcinoma.NELL1 and NELL2 protein expression is downregulated in clear cell renal carcinoma. PMID: 25726761
  11. NELL-1 demonstrates diffuse and reliable expression in benign but not malignant bone-forming skeletal tumors PMID: 25791475
  12. NELL1 overexpression greatly enhanced the osteogenic differentiation and mineral synthesis of iPSC-MSCs on RGD-grafted CPC scaffold for the first time. PMID: 25220281
  13. Two single-nucleotide polymorphisms of the NELL1 gene may represent a novel mechanism underlying hydrochlorothiazide-induced adverse metabolic effects (meta-analysis). PMID: 23400010
  14. NELL1 is able to promote the osteogenic differentiation of periodontal ligament stem cells, which may be related to the downregulation of Msx2 expression. PMID: 22767336
  15. Activation of the JNK pathway is necessary to mediate terminal osteogenic different-iation of Saos-2 osteosarcoma cells by rhNELL-1. PMID: 22797704
  16. Data indicate that LvNELL1 infection promoted the osteogenic differentiation of hADSCs, and the effect was comparable with that of LvBMP2. PMID: 23017834
  17. SHH and NELL-1 directed signaling produced additive effects on the pro-osteogenic and antiadipogenic differentiation of adipose derived stem cells. PMID: 22264144
  18. Prediction of radiographic severity in Ankolysing Spondylitis based on clinical variables can be significantly improved by including SNPs at ADRB1 (rs1801253), NELL1 (rs8176785) and MHC (rs1634747, rs9270986, rs7451962 and rs241453) genes. PMID: 22495925
  19. These results identify one potential mechanism of action for rhNELL-1 induced osteogenesis and highlight a fundamental difference between NELL-1 and BMP-2 signaling. PMID: 22580275
  20. NELL-1 may produce functional cartilage with properties similar to native cartilage. PMID: 21902605
  21. Results suggest that Osterix is a direct transcriptional regulator with repressive effect on NELL-1 gene expression, contributing to a delicate balance of regulatory effects on NELL-1 transcription with Runx2. PMID: 21931789
  22. Nell-1 is a candidate growth factor able to induce pericyte osteogenic differentiation. PMID: 21615216
  23. the effects of NELL-1 on osteoblastic differentiation and proliferation are partly through binding to APR3 PMID: 21723284
  24. The osteogenic effects of NELL1 on femoral distraction osteogenesis, was investigated. PMID: 20959151
  25. a potent growth factor that is highly specific to the osteochondral lineage, and has demonstrated robust induction of bone in multiple in vivo models [review] PMID: 20647499
  26. Data indicate that NELL1 is a ubiquitous inflammatory bowel disease susceptibility locus. PMID: 17684544
  27. Runx2 directly binds to the osteoblast specific binding elements 2 elements and transactivates the human NELL-1 promoter. PMID: 17042739
  28. Promoter hypermethylation of NELL1 is a common, tissue-specific event in human EAC, occurs early during Barrett's-associated esophageal neoplastic progression, and is a potential biomarker of poor prognosis in early-stage EAC PMID: 17452981
  29. findings suggest that upon binding to a specific receptor NELL1 transduces an osteogenic signal through activation of certain Tyr-kinases associated with the Ras-MAPK cascade, and finally leads to the osteogenic differentiation PMID: 18082140
  30. Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18519826
  31. Transgenic mice overexpressing Nell-1 develop craniosynostosis. PMID: 12235118

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Subcellular Location Cytoplasm, Nucleus envelope, Secreted
Database Links

HGNC: 7750

OMIM: 602319

KEGG: hsa:4745

STRING: 9606.ENSP00000349654

UniGene: Hs.657172

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